A5091821099- Mosquito-borne diseases and control
- HIV Research and Treatment
- Glycosylation and Glycoproteins Research
- Toxoplasma gondii Research Studies
- Carbohydrate Chemistry and Synthesis
- Malaria Research and Control
- Enzyme Structure and Function
- Phytoplasmas and Hemiptera pathogens
- Invertebrate Immune Response Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Viral Infections and Outbreaks Research
- Virology and Viral Diseases
- Machine Learning in Bioinformatics
- Trypanosoma species research and implications
- Natural Antidiabetic Agents Studies
- Plant Reproductive Biology
- Agricultural pest management studies
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Computational Drug Discovery Methods
- Vector-borne infectious diseases
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Proteins in Food Systems
- Microbial Metabolites in Food Biotechnology
Indian Institute of Technology Bombay
2020-2024
Indian Institute of Technology Roorkee
2015-2020
Jaypee University of Information Technology
2011
Momordica charantia (Mc) seeds are widely used edible crop with high nutritional quality. The food and pharmaceutical industries use it as a natural anti-oxygenic agent. Herein, ~52 kDa protein, which is major part of seed proteome has been purified, biochemically characterized structure determined. MALDI-ESI-MS identified peptide fragments contig-deduced sequence suggested the protein to be homologous 7S globulins. crystal shows that bicupin fold similar globulins electron density for...
Chikungunya virus has emerged as one of the most important global arboviral threats over last decade. Inspite large scale morbidity, with long lasting polyarthralgia, so far no licensed vaccine or antiviral is available. CHIKV nsP2 protease crucial for processing viral nonstructural polypeptide precursor to release enzymes required replication, thus making it a promising drug target. In this study, high cell density cultivation (HCDC) Escherichia coli in batch process was carried out produce...
Plasmodium falciparum plasmepsin X (PfPMX), involved in the invasion and egress of this deadliest malarial parasite, is essential for its survival hence considered as an important drug target. We report first crystal structure PfPMX zymogen containing a novel fold prosegment. A unique twisted loop from prosegment arginine 244 mature enzyme inactivation; such mechanism, not previously reported, might be common apicomplexan proteases similar to PfPMX. The maturation occurs through cleavage at...
Chikungunya virus nsP2 replication protein is a cysteine protease, which cleaves the nonstructural nsP1234 polyprotein into functional components. The cleavage and processing of by protease essential for proliferation virus. Thus, ChikV promising target antiviral drug discovery. In this study, crystal structure C-terminal domain (PDB ID: 4ZTB) was used based identification rational designing peptidomimetic inhibitors against protease. interactions junction residues nsP3/4 in active site have...
2S albumin is a low-molecular-weight seed storage protein belonging to the prolamin superfamily. In present work small (WTA) of ~16 kDa has been purified from seeds Wrightia tinctoria. The WTA heterodimer with subunit ~5 and larger ~11 bridged together through disulphide bonds. exhibits deoxyribonucleases activity against closed circular pBR322 plasmid DNA linear BL21 genomic DNA. also showed antibacterial Morexalla catarrhalis. CD studies indicate high α-helical content in protein....
The amino acid-binding receptors, a component of ABC transporters, have evolved to cater different specificities and functions. Of particular interest are cystine-binding which shown broad specificity. In the present study, putative periplasmic protein from Candidatus Liberibacter asiaticus (CLasTcyA) was characterized. Analysis CLasTcyA sequence crystal structures in ligand-bound state revealed novel features comparison related proteins. One unique found structure positioning C-terminal...
Auxin levels are tightly regulated within the plant cell, and its storage in isolated cavity of proteins is a measure adopted by cells to maintain availability auxin. We report first crystal structure Wrightia tinctoria 11S globulin (WTG) complex with Indole-3-acetic acid (IAA), an auxin, at 1.7 Å resolution. WTG hexamers assemble as result stacking interaction between hydrophobic surfaces two trimers, leaving space for binding charged ligands. The bound auxin stabilized non-covalent...
Given a protein sequence, how can we identify whether it is metalloprotein or not? If is, which main functional class and subclasses belongs to? This an important biological question because they are closely related to the function of uncharacterized protein. Particularly, with avalanche sequences generated in post genomic era since conventional techniques time consuming expensive, highly desirable develop automated method by one get fast accurate answer these questions. Here, top-down...
Toxoplasma gondii, a worldwide prevalent parasite is responsible for causing toxoplasmosis in almost all warm-blooded animals, including humans. Golgi-resident T. gondii aspartic protease 5 (TgASP5) plays an essential role the maturation and export of effector proteins those modulate host immune system to establish successful infection. Hence, inhibiting this enzyme can be possible therapeutic strategy against toxoplasmosis. This first report detailed structural investigations TgASP5 mature...
Abstract Toxoplasma gondii , a worldwide prevalent parasite, is responsible for causing toxoplasmosis by infecting almost all warm-blooded animals, including humans. To establish successful infection, the parasite exports series of effector proteins which modulates host immune system; Golgi-resident T. aspartyl protease 5 ( Tg ASP5) plays an essential role in maturation and export these proteins. This first report detailed structural investigation ASP5 mature enzyme. Molecular modeling...
Abstract Plasmodium falciparum plasmepsin X ( Pf PMX), involved in the invasion and egress of this deadliest malarial parasite, is essential for its survival hence considered as an important drug target. We report first crystal structure PMX zymogen containing a novel fold prosegment. A unique twisted loop from prosegment arginine 244 mature enzyme are inactivation; such mechanism, not previously reported, might be common apicomplexan proteases similar to PMX. The maturation occurs through...