A5105520751- Ion Transport and Channel Regulation
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal motility and disorders
- Ion channel regulation and function
- Drug Transport and Resistance Mechanisms
- Barrier Structure and Function Studies
- Gastroesophageal reflux and treatments
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Diet and metabolism studies
- Biochemical Analysis and Sensing Techniques
- Connexins and lens biology
- Neurotransmitter Receptor Influence on Behavior
- Neuroendocrine regulation and behavior
- Ion Channels and Receptors
- Olfactory and Sensory Function Studies
- Pediatric Hepatobiliary Diseases and Treatments
- Neuropeptides and Animal Physiology
- Digestive system and related health
- Pancreatic function and diabetes
- Metabolism and Genetic Disorders
- RNA Interference and Gene Delivery
- Clinical Nutrition and Gastroenterology
- Adenosine and Purinergic Signaling
- Advanced Chemical Sensor Technologies
Hokkaido University
1998-2024
University of Shizuoka
2008-2021
Sendai Seiyo Gakuin College
2021
Hiratsuka City Hospital
2018
Sendai Shirayuri Women's College
2013-2017
Food & Nutrition
2000-2017
Keio University
2014-2015
Kyoto Pharmaceutical University
2005-2011
Kyoto University
2005-2011
Physiol (Belgium)
2004
Microfluidic devices are widely used in lipid nanoparticle (LNP)-based vaccines and nanomedicine research. These should be stiff enough to withstand the high flow rate for mass production of LNPs, malleable use when fabricating complicated microchannel or micromixer structures, such as staggering herringbone micromixers. Due limitations available fabrication methods, optimal microfluidic have not yet been developed. In this study, we report development a glass-based device based on invasive...
Poly(N-isopropylacrylamide) (PNIPAAm)-based temperature-responsive fluorescence polymer probes were developed using radical polymerization, with 3-mercaptopropionic acid as the chain-transfer agent, followed by activation of terminal carboxyl groups N-hydroxysuccinimide and reaction 5-aminofluorescein (FL). The lower critical solution temperatures (LCSTs) resulting fluorescent differed depending on copolymer composition, had a sharp phase-transition (hydrophilic/hydrophobic) boundary at...
Loss of gastric acid secretion is pathologically known as achlorhydria. Acid-secreting parietal cells are characterized by abundant expression ezrin (Vil2), one ezrin/radixin/moesin proteins, which generally cross-link actin filaments with plasma membrane proteins. Here, we show the direct in vivo involvement secretion. Ezrin knockout (Vil2−/−) mice did not survive >1.5 wk after birth, making difficult to examine We then generated knockdown (Vil2kd/kd) introducing a neomycin...
Although paracellin-1 (PCLN-1) is known to have a crucial role in the control of Mg2+ reabsorption kidney, molecular pathways involved regulation PCLN-1 not been clarified. We used FLAG-tagged investigate these further, and found that phosphorylated at Ser217 by protein kinase A (PKA) under physiological conditions Madin-Darby canine kidney (MDCK) cells. expression decreased Na+ permeability, resulting decrease transepithelial electrical resistance (TER). By contrast, enhanced transport. PKA...
Paracellin-1 (PCLN-1) belongs to the claudin family of tight junction proteins and possibly plays a critical role in reabsorption magnesium calcium. So far, physiological properties PCLN-1 have not been clarified. In present study, we investigated whether is associated with ZO-1. We also (45)Ca(2+) transport across paracellular barrier affected by this association. vitro binding analysis using glutathione S-transferase fusion protein showed that C-terminal TRV sequence, especially Thr Val...
Abstract Several lines of evidence have suggested that the glutamatergic system in nucleus accumbens (NAc) plays an important role conditioned rewarding effect drugs abuse. In addition, it is recognized extracellular glutamate rapidly removed from synaptic cleft by Na + ‐dependent transporters neurons and glial cells, thereby maintaining physiological levels glutamate. We previously reported activation uptake a transporter activator attenuated acquisition place preference induced...
Repeated intermittent exposure to psychostimulants and morphine leads progressive augmentation of its locomotor activating effects in rodents. Accumulating evidence suggests the critical involvement mesocorticolimbic dopaminergic neurons, which project from ventral tegmental area nucleus accumbens medial prefrontal cortex, behavioral sensitization. Here, we examined acute chronic on dopamine release a reconstructed system comprised rat triple organotypic slice co-culture area, cortex...
The delivery of mRNA molecules to organs beyond the liver is valuable for therapeutic applications. Functionalized lipid nanoparticles (LNPs) using exogenous mechanisms can regulate
Theanine, L-N-ethylglutamine, is one of the major components amino acids in Japanese green tea. To characterize mode for intestinal absorption theanine, ionic dependency and kinetic properties theanine- glutamine-evoked transmural electrical potential difference changes (delta PD) were investigated vitro by using everted sacs prepared from guinea pig ileum. Both theanine glutamine applied to luminal side induced dose-dependent increases delta PD (increase serosal positive value). The values...
A guinea pig cDNA encoding the putative colonic H + -K -ATPase α-subunit (T. Watanabe, M. Sato, K. Kaneko, T. Suzuki, Yoshida, and Y. Suzuki; GenBank accession no. D21854 ) was functionally expressed in HEK-293, a human kidney cell line. The for cotransfected with either rabbit gastric or TorpedoNa β-subunit. In both expressions, Na -independent, K -dependent ATPase (K -ATPase) activity detected membrane fraction of cells, Michaelis-Menten constant 0.68 mM. inhibited by ouabain, its IC 50...
Two minor components of solanapyrones D and E, phytotoxins from Alternaria solani, were isolated. The absolute stereochemistry the optical purity A determined by chemical transformation measurement CD spectrum. In addition, complete signal assignment 1H- 13C-NMR spectra D, their solution conformations studied. Feeding experiments with various isotopically labeled acetates methionine established biosynthetic pathway solanapyrones. phytotoxicity on lettuce seedlings is discussed.
Abstract Intestinal brush-border membrane transport of monocarboxylic acids was investigated by using rabbit intestinal vesicles (BBMVs) and isolated tissues mounted on Ussing-type chambers. [3H]Mevalonic acid uptake BBMVs showed an overshoot phenomenon in the presence inwardly directed proton gradient, but not sodium gradient or outwardly HCO3− chloride gradient. Initial mevalonic saturable a Uptake [3H]mevalonic inhibited various acids, including acetic acid, benzoic lactic nicotinic...
To examine the effect of menstrual cycle on ventilatory sensitivity to rising body temperature, ten healthy women exercised for ~60 min a ergometer at 50% peak oxygen uptake during follicular and luteal phases their cycle. Esophageal mean skin minute ventilation, tidal volume were all significantly higher baseline exercise in phase than phase. On other hand, end-tidal partial pressure carbon dioxide was lower Plotting parameters against esophageal temperature revealed there be no significant...
One of the critical factors that determines individual differences in dietary behavior and nutritional status is sensory-affecting quality food, particular its taste. Variation one bitter taste receptor gene, TAS2R38, which associated with differential sensitivity to phenylthiocarbamide (PTC) 6-n-propylthiouracil (PROP), has been demonstrated affect intake pattern. A case study was performed examine association TAS2R38 genotypes/haplotypes body size (height, weight BMI) food nutrient intake....