A5050947712- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Cellular Mechanics and Interactions
- Cancer Genomics and Diagnostics
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Nerve injury and regeneration
- Axon Guidance and Neuronal Signaling
- Advanced Fluorescence Microscopy Techniques
- Hippo pathway signaling and YAP/TAZ
- Lipid Membrane Structure and Behavior
- Lung Cancer Treatments and Mutations
- Modular Robots and Swarm Intelligence
- Breast Cancer Treatment Studies
- Animal and Plant Science Education
- Science Education and Perceptions
- Neuroinflammation and Neurodegeneration Mechanisms
- Career Development and Diversity
- Neurobiology and Insect Physiology Research
- RNA Research and Splicing
- Computational Drug Discovery Methods
- Neurogenesis and neuroplasticity mechanisms
- Genetic factors in colorectal cancer
- Infrared Target Detection Methodologies
University of Maryland, College Park
2019-2023
National Institute of Neurological Disorders and Stroke
2019-2023
Rutgers, The State University of New Jersey
2015-2023
National Institutes of Health
2019-2023
Physical Sciences (United States)
2022
Cancer Council Queensland
2014-2015
The University of Queensland
2013
Cancer is a devastating disease that takes the lives of hundreds thousands people every year. Due to heterogeneity, standard treatments, such as chemotherapy or radiation, are effective in only subset patient population. Tumors can have different underlying genetic causes and may express proteins one versus another. This inherent variability cancer lends itself growing field precision personalized medicine (PPM). There many ongoing efforts acquire PPM data order characterize molecular...
Determining the shape of cell-specific dendritic arbors is a tightly regulated process that occurs during development. When this regulation aberrant, which disease or injury, alterations in result changes to neural circuitry. There has been significant progress on characterizing extracellular and intrinsic factors regulate dendrite number by our laboratory others. Generally, arbor are assessed Sholl analysis simple counting. However, we have found general method often overlooks local arbor....
This study explores collective learning in living neural networks, focusing on group-to-group Hebbian learning, i.e., strengthening and weakening of links dependent the precise timing their activities. While neuronal plasticity is now well understood for single pairs neurons, recent research has demonstrated that groups tens neurons are required to encode information mammalian brains. Thus, it critical understand how mechanisms plasticity, particular spike-timing-dependent (STDP) operate at...
Diffraction-limited and super-resolution imaging of biological samples using refractive-index matched polymers in microdevices.
The patterning of dendrites is regulated by many factors, such as brain-derived neurotrophic factor (BDNF), which our laboratory has previously shown alters the dendritic arbor uniquely depending on mode extracellular application. In current work, we examine how BDNF affects dendritogenesis in hippocampal neurons when it overexpressed intracellularly transcripts reported to be transported distinct cellular compartments. gene processed at two different polyadenylation sites, leading mRNA...
Astrocytes are key regulators of brain homeostasis, equilibrating ion, water, and neurotransmitter concentrations maintaining essential conditions for proper cognitive function. Recently, it has been shown that the excitability actin cytoskeleton manifests in second-scale dynamic fluctuations acts as a sensor chemophysical environmental cues. However, is not known whether excitable astrocytes how homeostatic function linked to dynamics cytoskeleton. Here regulation involves certain...
Abstract Plasticity and homeostatic mechanisms allow neural networks to maintain proper function while responding physiological challenges. Despite previous work investigating morphological synaptic effects of brain-derived neurotrophic factor (BDNF), the most prevalent growth in central nervous system, how exposure BDNF manifests at network level remains unknown. Here we report that treatment affects rodent hippocampal dynamics during development recovery from glutamate-induced...
Objective. This study investigates the effect that overexpression of cytosolic PSD-95 interactor (cypin), a regulator synaptic protein localization and core dendrite branching, exerts on electrical activity rat hippocampal neurons networks. Approach. We cultured used lipid-mediated transfection lentiviral gene transfer to achieve high levels cypin or mutant (cypinΔPDZ; non-binding) expression cellularly network-wide, respectively. Main results. Our analysis revealed although cypinΔPDZ...
Ena/VASP proteins are processive actin polymerases that required throughout animal phylogeny for many morphogenetic processes, including axon growth and guidance. Here we use in vivo live imaging of morphology distribution to determine the role Ena promoting TSM1 Drosophila wing. Altering activity causes stalling misrouting TSM1. Our data show has a substantial impact on filopodial this cone but exerts only modest effects distribution. This is contrast main regulator Ena, Abl tyrosine...
Cytosolic PSD-95 interactor (cypin) regulates many aspects of neuronal development and function, ranging from dendritogenesis to synaptic protein localization. While it is known that removal postsynaptic density protein-95 (PSD-95) the decreases N-methyl-D-aspartate (NMDA) receptors cypin overexpression protects neurons NMDA-induced toxicity, little about cypin’s role in AMPA receptor clustering function. Experimental work shows levels synaptosomes PSD, clusters/μm 2 , increases mEPSC...
Abstract Ena/VASP proteins are processive actin polymerases that required throughout animal phylogeny for many morphogenetic processes, including axon growth and guidance. Here we use live imaging of morphology organization in the TSM1 Drosophila wing to dissect mechanism Ena action. We find altering activity has a substantial impact on filopodial this cone, but exerts only modest effects organization. This is contrast main regulator Ena, Abl tyrosine kinase, which profound mild cone...
11092 Background: We undertook a prospective quality assurance study in 63 women with early breast cancer (EBC) identifying technical issues that required modification for real-time integration of MPS clinical and pathology parameters decision making. Methods: Pre-operatively, patients underwent 2 step formal informed consent process. Samples were collected fresh into Qiagen PaxGene Tissue Container preserving both morphology biomolecules lysis tissues overnight. Day 3; DNA extraction was...
e22126 Background: We have developed proprietary, innovative methods for the identification of actionable aberrations using NGS malignant tissues. Prior to commencing prospective decisions based on these technologies, we established a series QA programs validate our entire pipeline, including a) target selection panels b) sequencing technology, c) informatic data analysis variant calling and CNV. Methods: Breast cancer patient cohort 50 patients. 45 patients (90%) underwent BCT, 5 (10%)...
Abstract We undertook a prospective quality assurance and metric study in 26 women with early breast cancer (EBC) order to identify issues that may interfere real-time integration of massive parallel sequencing (MPS) clinical pathology parameters for decision making. Pre-operatively, patients underwent formal informed consent process the aid bespoke multimedia (iPad) technology educate on a) logistical aims trial b) somatic hereditary genomics c) privacy off target (non-cancer) issues. On...
Abstract Waves and oscillations play a key role in the flow processing of information brain. Recent work has demonstrated that addition to electrical activity, biomechanical signaling can also be excitable thus capable self-sustaining waves. Here we measured dynamics actin polymerization neural precursor cells throughout their differentiation into populations neurons astrocytes. Fluorescence-based live-cell imaging allowed us analyze conjunction with calcium signals. Actin showed rhythmic...
Abstract The discovery that astrocytes are an active, rather than a passive, component of the brain has ushered in paradigm shift thinking about how processes information. Although mechanisms by which integrate information from neurons still debated, such discourse should not distract importance more completely understanding communicate via signals amongst themselves. This work aims to study different signal within their own networks. We investigate group calcium (Ca 2+ ) dynamics polygonal,...
Abstract We demonstrate diffraction-limited and super-resolution imaging through thick layers (tens-hundreds of microns) BIO-133, a biocompatible, UV-curable, commercially available polymer with refractive index (RI) matched to water. show that cells can be directly grown on BIO-133 substrates without the need for surface passivation use this capability perform extended time-lapse volumetric cellular dynamics 1) at isotropic resolution using dual-view light-sheet microscopy, 2) instant...