A5006618435- Neuroscience and Neural Engineering
- Silk-based biomaterials and applications
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Ion Channels and Receptors
- Electrospun Nanofibers in Biomedical Applications
- Connexins and lens biology
- Conducting polymers and applications
- Neural dynamics and brain function
- Ion Transport and Channel Regulation
- Bone Tissue Engineering Materials
- Neurobiology and Insect Physiology Research
- Graphene and Nanomaterials Applications
- Nerve injury and regeneration
- Layered Double Hydroxides Synthesis and Applications
- RNA Interference and Gene Delivery
- Anesthesia and Neurotoxicity Research
- Nanoplatforms for cancer theranostics
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Polymer Nanocomposite Synthesis and Irradiation
- Quantum Dots Synthesis And Properties
- Microwave Dielectric Ceramics Synthesis
- Polymer Surface Interaction Studies
- Neuroinflammation and Neurodegeneration Mechanisms
Institute of Organic Synthesis and Photoreactivity
2016-2025
National Research Council
2012-2024
Consorzio Roma Ricerche
2015-2021
National Institutes of Health
2020
Yale University
2020
Harvard University
2020
Rockefeller University
2020
Brigham and Women's Hospital
2020
Federation of American Societies for Experimental Biology
2020
Institute of Nanostructured Materials
2009-2018
Regulatory volume decrease (RVD) is a key mechanism for control that serves to prevent detrimental swelling in response hypo-osmotic stress. The molecular basis of RVD not understood. Here we show complex containing aquaporin-4 (AQP4) and transient receptor potential vanilloid 4 (TRPV4) essential astrocytes. Astrocytes from AQP4-KO mice astrocytes treated with TRPV4 siRNA fail respond hypotonic stress by increased intracellular Ca 2+ RVD. Coimmunoprecipitation immunohistochemistry analyses...
The polymodal transient receptor potential vanilloid 4 (TRPV4) channel, a member of the TRP channel family, is calcium-permeable cationic that gated by various stimuli such as cell swelling, low pH and high temperature. Therefore, TRPV4-mediated calcium entry may be involved in neuronal glia pathophysiology associated with disorders central nervous system, ischemia. TRPV4 has been recently found adult rat cortical hippocampal astrocytes; however, its role astrocyte still not defined. In...
Silk is a natural biocompatible material that can be integrated in variety of photonic systems and optoelectronic devices. The silk replication patterned substrates with features down to tens nanometers exploited realize highly transparent, mechanically stable, free-standing structures optical wavelength size. We demonstrate organic lasing from blue-emitting stilbene-doped film spin-coated onto one-dimensional distributed feedback grating (DFB). threshold lower than DFB lasers based on the...
Accumulating evidence indicate that the gap-junction inhibitor carbenoxolone (CBX) regulates neuronal synchronization, depresses epileptiform activity and has a neuroprotective action. These CBX effects do not depend solely on its ability to inhibit gap junction channels formed by connexins (Cx), but underlying mechanisms remain be elucidated. Here we addressed questions whether modulates volume-regulated anion (VRAC) involved in regulatory volume decrease associated release of excitatory...
Astroglial ion channels are fundamental molecular targets in the study of brain physiology and pathophysiology. Novel tools devices intended for stimulation control astrocytes channel activity therefore highly desirable. The interactions between biomaterials is also essential to minimize reactive astrogliosis, view development implantable functional devices. Here, growth rat primary neocortical on top a light sensitive, organic polymer film reported; by means patch‐clamp analyses, effect...
In this study, we investigated the influence of processing methods on chemo-physical properties silk fibroin (SF) film and their interaction with neural cells.
Consolidated evidence indicates that astroglial cells are critical in the homeostatic regulation of cellular volume by means ion channels and aquaporin-4. Volume-regulated anion channel (VRAC) is chloride activated upon cell swelling critically contributes to astrocytes. The molecular identity VRAC has been recently defined, revealing it belongs leucine-rich repeat-containing 8 (LRRC8) protein family. However, there a lack demonstrating LRRC8A underpins currents astrocyte. Nonetheless,...
This work describes the development of electroconductive hydrogels as injectable matrices for neural tissue regeneration by exploiting a biocompatible conductive polymer - poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) combined with biomimetic network made gelatin. Our approach involved also genipin natural cross-linking agent to promote gelation gelatin networks embedding PEDOT:PSS. The achieved results suggest that physical-chemical properties resulting hydrogels, like...
Astrocytes are responsible for maintaining homoeostasis and cognitive functions through calcium signalling, a process that is altered in brain diseases. Current bioelectronic tools designed to study neurons not suitable controlling signals astrocytes. Here, we show electrical stimulation of astrocytes using electrodes coated with graphene oxide reduced induces respectively slow response calcium, mediated by external influx, sharp one, exclusively due release from intracellular stores. Our...
Abstract In the brain, astroglial syncytium is crucially involved in regulation of water homeostasis. Accumulating evidence indicates that a dysregulation astrocytic processes controlling homeostasis has pathogenetic role several brain injuries. Here, we have analysed by RNA interference technology functional interactions occurring between most abundant channel aquaporin‐4 (AQP4), and swelling‐activated Cl – current expressed cultured rat cortical astrocytes. We show primary astrocytes...
Abstract Silk fibroin (SF) is a biocompatible and slowly biodegradable material with excellent mechanical properties huge potential for use as biofunctional interface in electronic devices that aim to stimulate control neural network activity peripheral nerve repair. It shown SF films act interfaces support the adherence neurite outgrowth of dorsal root ganglion (DRG) neurons preserve neuronal functions. capability cells fire DRG on silk retain intracellular free Ca 2+ concentration ([Ca ] i...
Silk fibroin (SF), a protein core fibre from the silkworm Bombyx mori, has huge potential to become sustainable, biocompatible, and biodegradable material platform that can pave way towards replacement of plastic in fabrication bio-derived materials for variety technological biomedical applications. SF remarkable mechanical flexibility, controllable biodegradability, biocompatibility is capable drug/doping inclusion, stabilization release. However, dielectric properties limit its as direct...
Astrocytes are non-neuronal cells that govern the homeostatic regulation of brain through ions and water transport, Ca
Prostate cancer (PC) represents the most common type of among males and is second leading cause death in men Western society. Current options for PC therapy remain unsatisfactory, since they often produce uncomfortable long-term side effects, such as impotence (70%) incontinence (5-20%) even first stages disease. Light-triggered therapies, photodynamic therapy, have potential to provide important advances treatment localized partially metastasized prostate cancer. We designed a novel...
BACKGROUND/AIMS: The ability of astrocytes to control extracellular volume homeostasis is critical for brain function and pathology. Uncovering the mechanisms cell regulation by will be important identifying novel therapeutic targets neurological conditions, such as those characterized imbalances hydro saline challenges (as in edema) or altered glioma). One major challenge studying astroglial membrane channels involved culture model systems that expression patterns these do not resemble...