A5084623504- Chronic Lymphocytic Leukemia Research
- Probiotics and Fermented Foods
- Gut microbiota and health
- Retinoids in leukemia and cellular processes
- Galectins and Cancer Biology
- Helicobacter pylori-related gastroenterology studies
- Ubiquitin and proteasome pathways
- Inflammatory Bowel Disease
- Glycosylation and Glycoproteins Research
- Pharmacological Effects of Natural Compounds
- Diet, Metabolism, and Disease
- Asthma and respiratory diseases
- Microscopic Colitis
- Protease and Inhibitor Mechanisms
- Food Allergy and Anaphylaxis Research
- Papaya Research and Applications
- Advanced Glycation End Products research
- Cell Adhesion Molecules Research
- Digestive system and related health
- Signaling Pathways in Disease
- Infant Nutrition and Health
- Macrophage Migration Inhibitory Factor
- Bioactive natural compounds
- Ginseng Biological Effects and Applications
- Angiogenesis and VEGF in Cancer
Centro de Investigaciones Biológicas Margarita Salas
2012-2019
Consejo Superior de Investigaciones Científicas
2012-2019
Foundation for Biomedical Research
2013
Universidad de Granada
2006-2012
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2007-2011
The intestinal anti-inflammatory effects of two probiotics isolated from breast milk, Lactobacillus reuteri and L. fermentum, were evaluated compared in the trinitrobenzenesulfonic acid (TNBS) model rat colitis. Colitis was induced rats by intracolonic administration 10 mg TNBS dissolved 50% ethanol (0.25 ml). Either or fermentum daily administered orally (5 x 10(8) colony-forming units suspended 0.5 ml skimmed milk) to each group (n 10) for 3 weeks, starting 2 weeks before colitis...
The intestinal anti-inflammatory effects of three probiotics with immunomodulatory properties, Lactobacillus casei, acidophilus and Bifidobacterium lactis, were evaluated compared in the trinitrobenzenesulphonic acid (TNBS) model rat colitis.Colitis was induced rats by intracolonic administration 10 mg TNBS dissolved 0.25 ml 50% ethanol. Each probiotic administered orally (5x10(8) CFU suspended 0.5 skimmed milk) for 3 weeks, starting 2 weeks before TNBS. Colonic damage histologically...
Activation of nuclear hormone receptor peroxisome proliferator-activated β/δ (PPARβ) has been shown to improve insulin resistance and plasma high-density lipoprotein levels, but nothing is known about its effects in genetic hypertension. We studied whether the PPARβ agonist GW0742 might exert antihypertensive spontaneously hypertensive rats (SHRs). The were divided into 4 groups, Wistar Kyoto rat-control, rat-treated (GW0742, 5 mg · kg(-1) day(-1) by oral gavage), SHR-control, SHR-treated,...
Escherichia coli Nissle 1917 is a probiotic strain used in the treatment of intestinal immune diseases, including ulcerative colitis. The aim present study was to test if this bacterium can also show systemic immunomodulatory properties after oral administration.
The present study analysed the effects of flavanol ( − )-epicatechin in rats after chronic inhibition NO synthesis with N G -nitro- l -arginine methyl ester -NAME), at doses equivalent to those achieved studies involving human subjects. Wistar were randomly divided into four groups: (1) control-vehicle, (2) -NAME, (3) -NAME-epicatechin 2 -NAME-Epi 2) and (4) 10 10). Rats daily given by oral administration for 4 weeks: vehicle, or mg/kg. Animals -NAME groups received 75 mg/100 ml...
In the present study we describe preparation and chemical characterization of a caramel with high (70%) content difructose dianhydrides (DFAs) glycosylated derivatives (DFAs). This product was obtained by thermal activation (90 degrees C) highly concentrated (90% w/v) aqueous D-fructose solutions using sulfonic acid ion-exchange resin Lewatit S2328 as caramelization catalyst. DFAs represent unique family cyclic fructans prebiotic properties already in low proportions (<15%) commercial...
The preventative effects of the probiotic Lactobacillus fermentum CECT5716 were evaluated in lipopolysaccharide (LPS) model septic shock mice. was administered suspended drinking water at final concentration 10 8 colony-forming units/ml for 2 weeks before induction an endotoxic by intraperitoneal injection LPS (400 μg/200 μl per mouse). Blood and different organs collected after 24 h to evaluate severity probiotic. L. reduced TNF-α levels blood, which promotes major alterations observed...
We previously showed that pro-matrix metalloproteinase-9 (proMMP-9) binds to B chronic lymphocytic leukemia (B-CLL) cells and contributes B-CLL progression by regulating cell migration survival. Induction of survival involves a non-proteolytic mechanism the proMMP-9 hemopexin domain (PEX9). To help design specific inhibitors proMMP-9-cell binding, we have now characterized interaction with isolated PEX9. bound soluble immobilized GST-PEX9, but not GST, binding was mediated α4β1 integrin. The...
Dersalazine sodium (DS) is a new chemical entity formed by combining, through an azo bond, potent platelet activating factor (PAF) antagonist (UR-12715) with 5-aminosalicylic acid (5-ASA). DS has been demonstrated to have anti-inflammatory effects on trinitrobenzene sulphonic (TNBS)-induced colitis in rats and recently UC patients phase II PoC. There Increasing evidence that Th17 cells important role the pathogenesis of inflammatory bowel disease (IBD). The aim this study was further...
Myeloma cell adhesion dependent on α4β1 integrin is crucial for the progression of multiple myeloma (MM). The α4β1-dependent up-regulated by chemokine CXCL12, and pharmacological blockade CXCL12 receptor CXCR4 leads to defective homing bone marrow (BM). Sphingosine-1-phosphate (S1P) regulates immune trafficking upon binding G-protein-coupled receptors. Here we show that cells express S1P1, a S1P. We found S1P α4β1-mediated transendothelial migration stimulated CXCL12. promoted generation...
This study addresses the role of (pro)MMP-9 overexpression in CLL cell migration. We have used primary cells and CLL-derived MEC-1 transfected with empty (mock cells) or proMMP-9-encoding (MMP-9 lentiviral vectors. The constitutive expression mock was similar, whereas MMP-9 cells, resembled that incubated proMMP-9. In xenograft models, NOD/SCID mice, MMP-9-MEC-1 transfectants showed significantly reduced homing to bone marrow spleen compared cells. Likewise, incubation proMMP-9, before...
// Irene Amigo-Jiménez 1 , Elvira Bailón Noemí Aguilera-Montilla María José Terol 2 A. García-Marco 3 Angeles García-Pardo Cellular and Molecular Medicine Department, Centro de Investigaciones Biológicas, Consejo Superior Científicas (CSIC), Madrid, Spain Hematology Hospital Clínico Universitario, Valencia, Cytogenetics Unit, Instituto Investigación Sanitaria Puerta Hierro-Majadahonda, Correspondence to: García-Pardo, e-mail: agarciapardo@cib.csic.es Keywords: CLL, stromal cells, arsenic...
2-Hydroxy-4(-2,2,3,3,3-pentafluoropropoxy)-benzoic acid (UR-1505), a new molecule chemically related to salicylic acid, has immunomodulator properties and is currently under clinical development for treatment of atopic dermatitis. The present work describes the immunomodulatory profile UR-1505. UR-1505 targets T cells, inhibiting their proliferation cytokine production by blocking nuclear factor activated cells (NF-AT) DNA-binding activity. effects (100-300 μM) on cell seems be dependent...
Background Matrix metalloproteinase-9 (MMP-9) contributes to chronic lymphocytic leukemia (CLL) pathology by regulating cell migration and preventing spontaneous apoptosis. It is not known if MMP-9 involved in CLL response chemotherapy we address this the present study, using arsenic trioxide (ATO) fludarabine as examples of cytotoxic drugs. Methods We used primary cells from peripheral blood patients MEC-1 stably transfected with an empty vector or a containing MMP-9. The effect ATO was...