Introduction Although solventogenic Clostridium species (SCS) produce butanol, achieving high enough titers to warrant commercialization of biobutanol remains elusive. Thus, deepening our understanding the intricate cellular wiring SCS is crucial unearthing new targets and strategies for engineering novel strains capable producing tolerating greater concentrations butanol. Methods This study investigated potential role cyclic-di-adenosine monophosphate (c-di-AMP) in regulating solvent...

Staphylococcus aureus is an opportunistic bacterium of the human body and a leading cause nosocomial infections. Methicillin resistant S. (MRSA) infections involving biofilm lead to higher mortality morbidity in patients. Biofilm causes serious clinical issues, as it mitigates entry antimicrobials reach etiological agents. It plays important role resilient chronic which place unnecessary burden on antibiotics associated costs. To combat drug-resistant infection biofilm, there need discover...

Abstract Simple Sequence Repeats (SSRs) of polypurine-polypyrimidine type motifs occur very frequently in the 5′ flanks genes plants and have recently been implicated to a role regulation gene expression. In this study, 2 accessions Catharanthus roseus having (CT) 8 21 varying 5′UTR Tryptophan decarboxylase ( Tdc ) gene, were investigated for its Extensive expression analysis was carried out both at level transcription translation. Transcript abundance estimated using Northern qRT-PCR,...

ABSTRACT In denitrifying bacteria, nitric oxide (NO) is an electron acceptor and a free intermediate produced during anaerobic respiration. NO also signal for transcriptional regulation of the genes encoding nitrite (Nir), (Nor) nitrous reductases (N 2 OR). We hypothesise that timing strength necessary full nir expression are key factors in bet‐hedging strategy Paracoccus denitrificans , systems scavenging under hypoxia reduce probability induction. show flavohemoglobin Hmp scavenges aerobic...

In this study, a global response analysis was performed to explore the mechanism of action Usnic acid and its synergy with Norfloxacin, well-known quinolone antibiotic which MRSA clinical isolates showed resistance (MIC, 500 µg/mL). A microdilution assay, growth kinetics analysis, microscopic cell-based assays consistently that possesses strong anti-staphylococcal activity 7.8 µg/mL), causes cell leakage, modulates efflux pump activity, synergizes Norfloxacin against multi-drug-resistant...

Catharanthus roseus (L.) G. Don produces a number of biologically active terpenoid indole alkaloids via complex alkaloid biosynthetic pathway. The final dimerization step this pathway, leading to the synthesis dimeric alkaloid, vinblastine, was demonstrated be catalyzed by basic peroxidase. However, reports gene encoding enzyme are scarce for C. roseus. We report here first time cloning, characterization and localization novel peroxidase, CrPrx, from A 394 bp partial peroxidase cDNA ( CrInt1...

Bacteria belonging to the Alphaproteobacteria normally harbour multiple copies of heat shock sigma factor (known as σ(32), σ(H) or RpoH). Azospirillum brasilense, a non-photosynthetic rhizobacterium, harbours five rpoH genes, one which is an rpoH2 homologue. The genes around locus in A. brasilense show synteny with that found rhizobia. was able complement temperature-sensitive phenotype Escherichia coli mutant. Inactivation results increased sensitivity methylene blue and triphenyl...

Azospirillum brasilense, a plant-growth-promoting rhizobacterium, is exposed to changes in its abiotic environment, including fluctuations temperature, salinity, osmolarity, oxygen concentration and nutrient concentration, the rhizosphere soil. Since extra-cytoplasmic function (ECF) sigma factors play an important role stress adaptation, we analysed of ECF factor (also known as RpoE or σ(E)) tolerance A. brasilense. An in-frame rpoE deletion mutant brasilense Sp7 was carotenoidless...

Efficient bioconversion of abundant waste glycerol to value-added chemicals calls for a wider range fermentative workhorses that can catabolize glycerol. In this study, we used quantitative gene expression and solvent profiling, qualitative metabolite analysis, enzyme activity assays investigate the factors limit utilization as sole carbon source by Clostridium beijerinckii NCIMB 8052. C. 8052 did not produce acetate, acetone butanol on Congruently, genes encoding coenzyme A transferase...

The genome of the denitrifying bacterium Paracoccus denitrificans predicts expression a small heme-containing nitric oxide (NO) binding protein, H-NOX. organization and prior work in other bacteria suggest that H-NOX interacts with diguanylate cyclase cyclizes GTP to make cyclic di-GMP (cdGMP). Since cdGMP frequently regulates attached growth as biofilm, we first established conditions for biofilm development by P. denitrificans. We found adhesion polystyrene surface is strongly stimulated...

Enterobacter hormaechei is part of the cloacae complex (ECC), which widespread in nature. It a facultative Gram-negative bacterium medical and industrial importance. We assessed metabolic genetic repertoires new isolate. Here, we report whole-genome sequence furfural- 5-hydroxymethyl furfural (HMF)-tolerant strain E. (UW0SKVC1), uses glucose, glycerol, xylose, lactose arabinose as sole carbon sources. This exhibits high tolerance to (IC50 = 34.2 mM; ~3.3 g/L) relative Escherichia coli DH5α...

Extracytoplasmic function (ECF) sigma factors (σ(E)) are known to bring about changes in gene expression enable bacteria adapt different stresses. The Azospirillum brasilense Sp245 genome harbours nine genes encoding σ(E), of which two adjacent the ChrR-type zinc-binding anti-sigma (ZAS) factors. We describe here role and regulation a new pair rpoE-chrR, was found A. Sp7 addition previously described rpoE-chrR (designated rpoE1-chrR1). rpoE2-chrR2 is also cotranscribed, their products show...

Ever since the discovery of role bacteriophytochrome (BphP) in inducing carotenoid synthesis Deinococcus radiodurans response to light BphPs other non-photosynthetic bacteria is not clear yet. Azospirillum brasilense, a rhizobacterium, harbours pair out which AbBphP1 homolog AtBphP1 Agrobacterium tumefaciens. By overexpression, purification, biochemical and spectral characterization we have shown that photochromic bacteriophytochrome. Phenotypic study ΔAbBphP1 mutant showed it required for...

Bacteria must acquire the essential element zinc from extremely limited environments, and this function is performed largely by ATP binding cassette (ABC) transporters. These systems rely on a periplasmic or extracellular solute protein (SBP) to bind specifically with high affinity deliver it membrane permease for import into cytoplasm. However, acquisition in bacteria may be more complex, involving multiple transporters other proteins. Here we describe functions of two SBPs (ZnuA AztC)...

Carotenoids constitute an important component of the defense system against photooxidative stress in bacteria. In Azospirillum brasilense Sp7, a nonphotosynthetic rhizobacterium, carotenoid synthesis is controlled by pair extracytoplasmic function sigma factors (RpoEs) and their cognate zinc-binding anti-sigma (ChrRs). Its genome harbors two copies gene encoding geranylgeranyl pyrophosphate synthase (CrtE), first critical step biosynthetic pathway Inactivation each crtE paralogs found A....

Azospirillum brasilense harbors two redox-sensitive Zinc-binding anti-sigma (ZAS) factors (ChrR1 and ChrR2), which negatively regulate the activity of their cognate extra-cytoplasmic function (ECF) σ (RpoE1 RpoE2) by occluding binding to core enzyme. Both pairs RpoE-ChrR control responses photooxidative stress. The aim this study was investigate whether cross-talk while responding stress.In silico analysis showed a high sequence similarity between ChrR1 ChrR2 proteins, but differences in...

The genome of Azospirillum brasilense encodes five RpoH sigma factors: two OxyR transcription regulators and three catalases. aim this study was to understand the role they play during oxidative stress their regulatory interconnection. Out 5 paralogs present in A. brasilense, inactivation only rpoH1 renders heat sensitive. While transcript levels were elevated by stress, those rpoH3 rpoH5 upregulated H2O2 Catalase activity its rpoH::km mutants response except case rpoH5::km mutant,...

We report the complete genome sequence of Paenibacillus polymyxa DSM 365. The consists a 5,788,318-bp chromosome, with GC content 45.48%. Annotation revealed total 5,246 genes (average length, 943 bp). Gene function analysis indicated ability to fix nitrogen (N2) and produce value-added chemicals.

Abstract Background The compound 1,2-propanediol is an important industrial bulk chemical that has proven particularly recalcitrant to bio-production. Solvent-producing Clostridium species represent promising candidates for engineering 1,2-propaediol production. Co-production of 1,2-popanediol and butanol the potential improve economics acetone-butanol-ethanol (ABE) fermentation. Results In this study, methylglyoxal synthase gene ( mgsA ) from beijerinckii NCIMB 8052 was homologously...