A5021418077- Animal Disease Management and Epidemiology
- Neonatal Health and Biochemistry
- Cancer therapeutics and mechanisms
- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Viral Infections and Immunology Research
- Pharmacological Effects of Natural Compounds
- Natural product bioactivities and synthesis
- Plant Virus Research Studies
- Nutrition, Genetics, and Disease
- T-cell and Retrovirus Studies
- Carcinogens and Genotoxicity Assessment
- Lung Cancer Research Studies
- DNA Repair Mechanisms
- Sexual Differentiation and Disorders
- Acute Lymphoblastic Leukemia research
- Prostate Cancer Treatment and Research
- Ginseng Biological Effects and Applications
- Herpesvirus Infections and Treatments
- Prostate Cancer Diagnosis and Treatment
- Vector-Borne Animal Diseases
- Animal Virus Infections Studies
- Medical Device Sterilization and Disinfection
- Musculoskeletal pain and rehabilitation
- Nuclear Structure and Function
Centre Hospitalier Universitaire de Lille
2023-2024
Université Laval
2004-2017
Institut Universitaire en Santé Mentale de Québec
2017
Centre hospitalier universitaire de Québec
2011
PharmacoGenetics (China)
2010
Centre hospitalier de l'Université Laval
2006
Institut de Génomique Fonctionnelle
1887
Objectives Polymorphisms in UDP-glucuronosyltransferases (UGTs) can influence detoxifying capacities and have considerable therapeutic implications addition to various (patho)physiological processes. UGT1A9 plays a central role the metabolism of classes drugs carcinogens steroids. The great interindividual variability UGT1A9-mediated glucuronidation remains poorly explained, while evidence for its genetic origin exists. Methods proximal promoter was screened polymorphisms by sequencing and,...
In vitro metabolic studies revealed that along with UDP-glucuronosyltransferase (UGT) 1A1, the hepatic UGT1A9 and extrahepatic UGT1A7 are involved in biotransformation of active toxic metabolite irinotecan, 7-ethyl-10-hydroxycamptothecin (SN-38). Variant UGT1A1 alleles have been reported but polymorphic nature <i>UGT1A9</i> gene has not yet. To further clarify molecular determinants irinotecan-induced toxicity, we identified characterized functionality novel polymorphisms determined whether...
UDP-glucuronosyltransferase 1A1 (UGT1A1) is involved in a wide range of biological and pharmacological processes because its critical role the conjugation diverse array endogenous exogenous compounds. We now describe new UGT1A1 isoform, referred to as isoform 2 (UGT1A1_i2), encoded by 1495-bp complementary DNA isolated from human liver generated an alternative splicing event involving additional exon found at 3' end UGT1A locus. The N-terminal portion 45-kd UGT1A1_i2 protein identical (55...
Background The gene UGT1 encodes phase II detoxification proteins involved in the elimination of small hydrophobic substances both endogenous and exogenous origin. To date, nine functional UGT1A are known to be produced from a single composed alternative first exons shared with four common exons. Recently, novel exon (referred as 5b) was identified region. Results We now reveal splicing mechanism demonstrate that 5a new 5b alternatively spliced, generating several variant mRNAs up previously...
PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-f]pyridine), the most abundant heterocyclic amine in diet, is involved etiology of cancer. and its carcinogenic metabolite N-hydroxy-PhIP (N-OH-PhIP) are extensively conjugated by UDP-glucuronosyltransferase (UGTs) with wide variability. This study aimed to determine genetic influence UGTs on hepatic detoxification this carcinogen. The formation N-OH-PhIP glucuronides was studied 48 human liver samples mass spectrometry. Liver were genotyped for...
Polymorphisms in <i>UGT1A9</i> were associated with reduced toxicity and increased response to irinotecan cancer patients. UDP-glucuronosyltransferase (UGT) protein expression, glucuronidation activities for 7-ethyl-10-hydroxycamptothecin (SN-38), probe substrates of the UGT1A9 UGT1A1 measured 48 human livers clarify role variants on vitro SN-38. Genotypes assessed (–2152C>T, –275T>A, –118T<sub>9>10</sub>), three novel (–5366G>T, –4549T>C, I399C>T), <i>UGT1A1</i>...
UDP-glucuronosyltransferase (UGT) enzymes catalyze the glucuronidation reaction, which is a major pathway in catabolism and elimination of numerous endo- xenobiotics. Among UGT enzyme family members, UGT1A7, UGT1A8, UGT1A9, UGT1A10 isoforms are issued from single gene through differential splicing. However, these display distinct tissue-specific expression patterns. Indeed, exclusively expressed extrahepatic tissues, whereas UGT1A9 transcripts found at high concentrations liver. In present...
The UDP-glucuronosyltransferase UGT1 locus is composed of nine exon 1s, each flanked by a unique promoter region, and common exons (2, 3, 4, the alternatively spliced 5a 5b). Here, we characterized genetic architecture gene in Caucasian sample. Overall, 98 variations regulatory domains, exon–intron boundaries were genotyped 254 unrelated subjects, including 12 unreported polymorphisms. We determined allele frequencies, computed pairwise linkage disequilibrium (LD), inferred haplotypes; this...
UDP-glucuronosyltransferases (UGTs) are major mediators in conjugative metabolism. Current data suggest that UGTs, which anchored the endoplasmic reticulum membrane, can oligomerize with each other and/or metabolic enzymes, a process may influence their enzymatic activities. We demonstrated previously <i>UGT1A</i> locus encodes unknown isoforms (denoted “i2”), by alternative usage of terminal exon 5. Although i2 proteins lack transferase activity, we showed knockdown endogenous levels...
UGT2B4 is a member of the UDP-glucuronosyltransferase (UGT) superfamily, major detoxifying system in humans. involved bile acids metabolism and highly expressed liver extrahepatic tissues. The aim this study was to uncover new molecular mechanisms underlying interindividual variability pathway.We carried out comprehensive scan for additional exons at locus discovered multiple alternative splicing events. We then assessed expression profile alternatively spliced transcripts human tissues...
Sporadic Alzheimer's disease (AD), as opposed to its autosomal dominant form, is likely caused by a complex interaction of genetic, environmental, and health lifestyle factors. Twin studies indicate that sporadic AD heritability could be between 58% 79%, around half which explained the ε4 allele apolipoprotein E (APOE4). We hypothesized genes associated with known risk factors for AD, namely hypertension, hypercholesterolemia, obesity, diabetes, cardiovascular disease, would contribute...
Taking into account the psychological impact to set Total Work Incapacity (TWI) is a difficult exercise not making consensus. Anterior works have described points of interest look for during forensic consultation. This work aims study global standardized scale utilization, Global-Clinic-Impression-Severity (CGI-S) and psychometric self-questionnaires utilization in psychotraumatic clinics, on legal medical practice. monocentric prospective conducted at University Hospital Lille, over period...
En résumé la vaccination contre peste bovine à l’aide de virus caprin ne saurait être trop recommandée. Elle présente sur les autres procédés : séro-infection, vaccino-infection - seuls qui vraiment aient fait leurs preuves des avantages certains d’économie, d’innocuité, d’efficacité, simplicité d’intervention.