A5017839254- Genomics, phytochemicals, and oxidative stress
- Fish Ecology and Management Studies
- Effects of Radiation Exposure
- Fish Biology and Ecology Studies
- Cancer therapeutics and mechanisms
- Glycosylation and Glycoproteins Research
- Marine and fisheries research
- Carbohydrate Chemistry and Synthesis
- Arctic and Russian Policy Studies
- Mass Spectrometry Techniques and Applications
- Immunotherapy and Immune Responses
- International Maritime Law Issues
- Glutathione Transferases and Polymorphisms
- DNA Repair Mechanisms
- Analytical Chemistry and Chromatography
- Aquaculture Nutrition and Growth
- Hydrology and Sediment Transport Processes
- vaccines and immunoinformatics approaches
- Aquatic Invertebrate Ecology and Behavior
- Neutropenia and Cancer Infections
- Indigenous Studies and Ecology
- Biological Control of Invasive Species
- Reproductive biology and impacts on aquatic species
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
IONICS Mass Spectrometry (Canada)
2011-2023
United Nations
2022
Naval War College
2015-2022
University of California, Los Angeles
2001-2021
BCN Biosciences (United States)
2017-2021
United States Coast Guard
2018-2019
Department of Agriculture, Fisheries and Forestry
2014
University of Virginia
2007-2013
Western Sydney University
2008-2011
St. Elizabeth's Medical Center
2010
Immunity against phosphopeptide antigens lacking in leukemia patients can be restored with stem cell transplantation.
The transcription factor NF-E2-related 2 (Nrf2) binds the antioxidant DNA response element (ARE) to activate important cellular cytoprotective defense systems. Recently several types of cancers have been shown overexpress Nrf2, but its role in radiation therapy has yet be fully determined. In this study, we report that single doses ionizing from 8 Gy ARE-dependent breast cancer cells a dose-dependent manner, only after delay five days. Clinically relevant daily dose fractions also increased...
The activation and recruitment of CD4(+) T cells are critical for the development efficient antitumor immunity may allow optimization current cancer immunotherapy strategies. Searching more optimal selective targets cells, we have investigated phosphopeptides, a new category tumor-derived epitopes linked to proteins with vital cellular functions. Although MHC I-restricted phosphopeptides been identified, it was previously unknown whether human II molecules present specific cell recognition....
Abstract Nitroxyl (HNO) can inhibit the glycolytic enzyme glyceraldehyde 3‐phosphate dehydrogenase (GAPDH). Because of importance glycolysis in many malignant cells, we thus propose that HNO adversely affect tumor growth. This hypothesis was tested using vitro and vivo models breast cancer. We report here for first time suppresses proliferation both estrogen receptor (ER)‐positive ER‐negative human cancer cell lines, a dose dependent manner. Mice treated with either injected into itself or...
Discovery of agents that protect or mitigate normal tissue from radiation injury during radiotherapy, accidents, terrorist attacks is importance. Specifically, bone marrow insufficiency, with possible infection due to immunosuppression, can occur after total body irradiation (TBI) regional and a major component the acute syndrome. The purpose this study was identify novel radioprotectors mitigators hematopoietic system.High-throughput screening small-molecule libraries done using viability...
Radiation-induced gastrointestinal syndrome (RIGS) results from the acute loss of intestinal stem cells (ISC), impaired epithelial regeneration, and subsequent mucosal barrier, resulting in electrolyte imbalance, diarrhea, weight loss, sepsis, mortality. The high radiosensitivity epithelium limits effective radiotherapy against abdominal malignancies survival victims nuclear accidents or terrorism. Currently, there is no approved therapy to mitigate radiation toxicity intestine. Here we...
Flow injection analysis with electrospray ionization mass spectrometry was used to investigate borate-nucleotide complex formation. Solutions containing 100 microM nucleotide and 500 boric acid in water-acetonitrile-triethylamine (50:50:0.2, v/v/v; pH 10.3) showed that borate complexation nicotinamide nucleotides significantly influenced by the charge on group number of phosphate groups adenine ribose. Borate binding decreased order NAD(+), NADH, NADP(+) NADPH. To relationship between...
Common carp (Cyprinus carpio) are one of the world's most destructive vertebrate pests. In Australia, they dominate many aquatic ecosystems causing a severe threat to plants, invertebrates, water quality, native fish and social amenity. The Australian Government is considering release cyprinid herpesvirus-3 (CyHV-3) as control measure consequently robust, continental-scale estimate population biomass essential inform planning risk management. Here, we pioneer novel model-based approach...
An accurate, rapid, and versatile method for the analysis of enzyme kinetics using electrospray ionization mass spectrometry (ESI-MS) has been developed demonstrated fucosyltransferase V. Reactions performed in primary or secondary amine-containing buffers were diluted an ESI solvent directly analyzed without purification reaction products. Decreased resolution was used to maximize instrument sensitivity, multiple monitoring (MRM), tandem spectrometric mode, enhance selectivity detection....
Purpose: An unbiased approach of drug discovery through high-throughput screening (HTS) libraries chemically defined and bioactive small molecule compounds was used to identify modulators radiation injury with an emphasis on radioprotectors mitigators rather than radiosensitisers. Assay system endpoints included radiation-induced genotoxicity DNA damage in yeast apoptosis murine lymphocytes. Large-scale data mining diverse identified agents that were effective all endpoints. HTS compound...
Investigators have shown that PC-SPES is a potent herbal mixture which has often been used by prostate cancer patients. In this study, we examined the inhibitory effects of certain individual components on in vitro proliferation human breast cells MDA-MB231 and umbilical vein endothelial (HUVEC). Our data showed had varying suppressive cellular proliferation, Rabdosia rubescens appeared to be most agent these assays. Apoptosis was up-regulated rubescens, as seen caspase-9 TUNEL These may...
The gene transfer agent of Rhodobacter capsulatus (GTA) is a unique phage-like particle that exchanges genetic information between members this same species bacterium. Besides being an excellent tool for mapping, the GTA has number advantages biotechnological and nanoengineering purposes. To facilitate purification identify proteins involved in expression, assembly regulation, present work we construct transform into R. Y262 coding C-terminally His-tagged capsid protein. constructed protein...
More and larger vessels are operating in the Arctic's northern Bering Sea Strait their associated waste streams pose a growing risk to ecosystem. These collective risks particularly concerning Indigenous people region, whose culture subsistence hinge upon preservation of pristine marine environment. This article describes ecological cultural significance waters people, then discusses these from increasing vessel traffic streams. The quantifies amount three principal – oil, sewage, grey water...
Our ability to use ionizing radiation as an energy source, a therapeutic agent, and, unfortunately, weapon, has evolved tremendously over the past 120 years, yet our tool box handle consequences of accidental and unwanted exposure remains very limited. We have identified novel group small molecule compounds with 4-nitrophenylsulfonamide (NPS) backbone in common that dramatically decrease mortality from hematopoietic acute syndrome (hARS). The emerged vitro high throughput screen (HTS) for...
Abstract We recently discovered a novel gene and named it endothelial-derived 1 (EG-1). Previously, we have shown that the expression of EG-1 is significantly elevated in epithelial cells breast cancer, colorectal prostate cancer. Here, report can stimulate cellular proliferation. Transfection experiments which overexpressed full-length human embryonic kidney HEK-293 or cancer cell lines resulted increased vitro proliferation, comparison with transfection empty vectors. On other hand, small...
EG-1 is significantly elevated in breast, colorectal, and prostate cancers. Overexpression of stimulates cellular proliferation, targeted inhibition blocks mouse xenograft tumor growth. To further clarify the function EG-1, we investigated its role c-Src activation. We observed that overexpression results activation c-Src, but found no evidence a direct Src substrate. also binds to other members family. Furthermore, shows interaction with multiple SH3- WW-containing molecules involved...
AbstractEG-1 is a gene product that significantly elevated in human breast cancer tissues. Previously, we have shown EG-1 overexpression stimulates cellular proliferation both vitro and vivo. Here, ask whether this molecule can be targeted for experimental therapeutic purpose. siRNA lentivirus polyclonal antibody were designed to suppress expression. These agents then used cell culture assays tumor xenograft models. Serum urine from patients also analyzed the presence of peptide. We report...
Green tea may prevent cancer, partially by inhibiting tumor angiogenesis. Our previous studies showed that green extract was effective in breast cancer and endothelial cell proliferation vitro, suppressed xenograft size decreased the vessel density vivo. Here, we set out to further investigate molecular mechanisms of this observed angiogenesis suppression. We utilized cDNA microarray technology profile global changes cellular gene expression response tea. HUVEC (human umbilical vein cells)...
Alligator weed is a serious in many countries. In Australia, it “weed of national significance” because its actual and potential impact. We surveyed all local governments New South Wales 2001 2007 to determine whether the being contained. found an increased number extent infestations, despite more resources better knowledge base. Most considered that further research needed tactics for control weed. On basis current containment urban gardens, we recommend mobilize community (e.g., bush...
‘Demonstration reaches’ are sections of river where multiple threats to native fish addressed through rehabilitation and strong community participation. They an important way promoting the key driving actions Murray-Darling Basin Authority's Native Fish Strategy (NFS) by using on-ground community-driven rehabilitation. Measuring success against well-defined targets this information adaptively mange activities is fundamental demonstration reach philosophy. Seven years on from establishment...
Context Following recent major fish kill events, there is increasing interest in stocking Murray cod fingerlings to help stocks recover. Strategies that can increase post-release survival of stocked are important for conservation and may also have application other species. Aims The aim this work was evaluate whether pre-release predator recognition avoidance training conferred a advantage on (Maccullochella peelii) fingerlings. Predator-free release enclosures were evaluated as method...