The late stages of age-related maculopathy (ARM), especially neovascular macular degeneration (ARMD), can severely affect central vision and are the main cause blindness in elderly Western world. It has been shown that angiogenic growth factors present membranes ARMD. However, it is not known if play a role onset neovascularisation.In order to elucidate involvement initiation neovascularisation early ARM, expression patterns VEGF, TGF-beta, b-FGF, PDGF-AA on 18 human maculae with 11 control...

Chronic airways inflammation is one of the features chronic obstructive pulmonary disease (COPD). We demonstrated previously that bronchiolar epithelium in COPD contains increased numbers macrophages and mast cells. Transforming growth factor β1 (TGF- β1) may be involved this influx because it has chemotactic activity for In study, we examined expression patterns TGF- β1, β receptors type I II RI RII) by immunohistochemistry mRNA situ hybridization peripheral lung tissue 14 current or...

Epithelial cells form a tight barrier against environmental stimuli via junctions (TJs) and adherence (AJs). Defects in TJ AJ proteins may cause changes epithelial morphology integrity potentially lead to faster trafficking of inflammatory through the epithelium. Bronchial fragility has been reported asthmatic patients, but little is known about expression asthma. We studied zonula occludens-1 (ZO-1) E-cadherin, alpha-catenin, beta-catenin bronchial biopsies from nonatopic nonasthmatic...

Ongoing inflammatory processes resulting in airway and vascular remodelling characterise chronic obstructive pulmonary disease (COPD). Vascular endothelial growth factor (VEGF) its receptors VEGFR-1 (Flt-1) VEGFR-2 (KDR/Flk-1) could play a role tissue angiogenesis COPD.The cellular expression pattern of VEGF, Flt-1, KDR/Flk-1 was examined by immunohistochemistry central peripheral lung tissues obtained from ex-smokers with COPD (forced expiratory volume 1 second (FEV(1)) <75% predicted; n =...

The molecular basis of myocardial adaptation to ischemia and reperfusion is poorly understood. It thought that nuclear proto-oncogenes act as third messengers, converting cytoplasmic signal transduction into long-term changes gene expression. We studied the expression six (Egr-1, c-fos, fosB, c-jun, junB, c-myc) in myocardium subjected anesthetized pigs. Stunning was achieved by two 10-minute left anterior descending coronary artery occlusions separated 30 minutes reperfusion. Hearts were...

Remodeling of airways and blood vessels is an important feature in chronic obstructive pulmonary disease (COPD). By using immunohistochemical analysis, we examined bronchial expression patterns various extracellular matrix (ECM) components such as collagens (subtypes I, III, IV), fibronectin, laminin β2 patients with COPD (forced expiratory volume 1 second [FEV1] ≤75%; n = 15) without (FEV1 =≥85%; 16) correlated data lung function. Quantitative analysis revealed enhanced levels (P < .01)...

Pulmonary hypoplasia accompanied by pulmonary hypertension resistant to treatment is an important feature of congenital diaphragmatic hernia (CDH). The pathogenesis the vascular abnormalities in CDH remains be elucidated at molecular level. Vascular endothelial growth factor (VEGF), cell specific mitogen, known play a role angiogenesis and remodelling but there are no data on VEGF expression patients with CDH.Necroscopic lung specimens from 21 seven age matched control newborn infants...

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Abstract An important feature of chronic obstructive pulmonary disease (COPD) is airway remodelling, the molecular mechanisms which are poorly understood. In this study, role fibroblast growth factors (FGF‐1 and FGF‐2) their receptor, FGFR‐1, was assessed in bronchial wall remodelling patients with COPD (FEV 1 &lt; 75%; n = 15) without &gt; 85%; 16). FGF‐1 FGFR‐1 were immunolocalized epithelium, smooth muscle (ASM), submucosal glandular vascular muscle. Quantitative digital image analysis...

Fresh bovine, porcine and canine hearts were homogenized mitogens for mesoderm‐derived cells purified in three different steps. Extraction by two ammonium sulfate precipitations was followed cation‐exchange chromatography heparin‐Sepharose affinity chromatography. A fraction from heart (eluted at 1.1 M NaCl) increased mitotic activity serum‐deprived cultures of aortic endothelial smooth muscle cells, human fibroblasts. This mitogenic is potentiated heparin inhibited γ‐interferon. The showed...

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Congenital heart disease (CHD) leading to increased pulmonary blood pressure and flow is an important cause of plexogenic arteriopathy (PPA). This type tends progress irreversible stage, hallmarked histologically by the emergence a number characteristic lesions, which include concentric laminar intimal proliferation fibrosis, plexiform lesions. The pathogenesis these connote very poor prognosis, not well understood. Since endothelial cell has been demonstrated in it was hypothesized that...

Mediators of myocardial inflammation, predominantly cytokines, have for many years been implicated in the healing processes after infarction. In recent years, however, more attention has paid to possibility that inflammation may result deleterious complications The proinflammatory cytokines mediate dysfunction associated with infarction, severe congestive heart failure, and sepsis. A growing body literature suggests inflammatory mediators could play a crucial role ischemia-reperfusion...