A5101744957- Catalytic C–H Functionalization Methods
- Synthesis and Catalytic Reactions
- Catalytic Cross-Coupling Reactions
- RNA Research and Splicing
- Photosynthetic Processes and Mechanisms
- Studies on Chitinases and Chitosanases
- Insect symbiosis and bacterial influences
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Asymmetric Hydrogenation and Catalysis
- Pharmacogenetics and Drug Metabolism
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Drug Transport and Resistance Mechanisms
- Cyclopropane Reaction Mechanisms
- Catalytic Processes in Materials Science
- Sulfur-Based Synthesis Techniques
- RNA Interference and Gene Delivery
- Traditional Chinese Medicine Analysis
- Signaling Pathways in Disease
- Nanomaterials for catalytic reactions
- Fluorine in Organic Chemistry
- Reproductive Biology and Fertility
- Diet, Metabolism, and Disease
- HIV/AIDS drug development and treatment
Hebei Medical University
2025
Second Hospital of Hebei Medical University
2025
Nanjing Drum Tower Hospital
2025
Soochow University
2014-2024
Beijing University of Chemical Technology
2024
Ministry of Agriculture and Rural Affairs
2023-2024
China Agricultural University
2021-2024
Guiyang Medical University
2020-2024
Changsha Medical University
2024
Westlake University
2023
More than 170 proteins are necessary for assembly of ribosomes in eukaryotes. However, cofactors that function with each these proteins, substrates on which they act, and the precise functions factors—e.g., recruiting other molecules into preribosomes or triggering structural rearrangements pre-rRNPs—remain mostly unknown. Here we investigated recruitment two ribosomal 5S RNA (rRNA) nascent ribosomes. We identified a ribonucleoprotein neighborhood contains yeast ribosome factors, Rpf2 Rrs1,...
Selective and fast: A flavylium derivative-based ratiometric fluorescent probe (1) for H2S is reported. The reaction mechanism (see scheme) based on the nucleophilic addition of towards electrically positive benzopyrylium moiety 1, which can efficiently differentiate from other competitive species. exhibits a fast response toward H2S, within 10 s, superior to most reported probes.
A Ru-catalyzed 1,1′-binaphthyl-2,2′-diylhydrogen phosphate (BNDHP)-enabled meta C–H benzylation under the assistance of ferrocene using less sterically hindered toluene derivatives as coupling partners has been developed. Various arenes bearing pyridyl, pyridmidyl, and pyrazolyl directing groups can be selectively coupled with toluenes at positions in moderate to good yield. mechanistic study clearly showed site selectivity ortho or position is completely controlled by ligand BNDHP catalyst...
Palladium-catalyzed direct ortho-silylation of oxalyl amide-protected phenylmethanamine and phenethylamine with commercially available disilanes is reported. Germanylation products were also produced under the same reaction conditions. This protocol tolerated aliphatic amines, which gave γ-C-H silylation products.
A protocol for palladium-catalyzed ortho C(sp2)–H alkenylation via a rarely reported seven-membered palladacycle with oxalyl amide as directing group was reported. The range of olefins the broadest yet this kind C–H reaction. For example, allyl acetate, alcohol derivatives, acraldehyde, acrylate and acrylonitrile were all tolerated in transformation. Sequential reactions also achieved to construct complex olefinated arenes good yields one pot.
Remdesivir (RDV; GS-5734, Veklury), the first FDA-approved antiviral to treat COVID-19, is a single-diastereomer monophosphoramidate prodrug of an adenosine analogue. RDV taken up in target cells and metabolized multiple steps form active nucleoside triphosphate (TP) (GS-443902), which, turn, acts as potent selective inhibitor viral RNA polymerases.
Marine and terrestrial photosynthesis exhibit a schism in the accessory chlorophyll (Chl) that complements function of Chl : b for green plants versus c most eukaryotic phytoplankton. The enzymes mediate biosynthesis have long remained elusive. In this work, we identified CHLC dioxygenase (Phatr3_J43737) from marine diatom Phaeodactylum tricornutum as synthase. chlc mutants lacked , instead accumulating its precursors, exhibited growth defects. vitro, recombinant protein converted these...
To better define the roles of assembly factors required for eukaryotic ribosome biogenesis, we have focused on one specific step in maturation yeast 60 S ribosomal subunits: processing 27SB pre-ribosomal RNA. At least 14 factors, 'B-factor' proteins, are this step. These include most major functional classes factors: RNA-binding scaffolding protein, DEAD-box ATPases and GTPases. We investigated mechanisms by which these associate with assembling ribosomes. Our data establish a recruitment...
An effective protocol for the synthesis of quinolinone derivatives by palladium-catalyzed sequential β-C(sp<sup>3</sup>)–H arylation and selective intramolecular C(sp<sup>2</sup>)–H/N–H amination is developed.
Saturated fatty acid (SFA) and unsaturated (UFA) have distinct impacts on health. Whether SFA UFA are differentially transported in liver remains elusive. Here, we find the secretion of but not esters is retarded a male mouse hepatic endoplasmic reticulum (ER) stress model. Among 13 members protein disulfide isomerase (PDI) family, only PDIA1 deficiency leads to hepatosteatosis hypolipidemia. In PDI-deficient liver, there severe accumulation secretory blockade esters, whereas remain normal....
<title>Abstract</title> <bold>Background</bold> Mitochondrial dysfunction in granulosa cells (GCs) has been implicated the pathogenesis of polycystic ovary syndrome (PCOS). Our previous studies showed that melatonin alleviated mitochondrial by upregulating SIRT1 (NAD-dependent deacetylase sirtuin-1) expression. Dynamin-related protein 1 (Drp1) mediated fission is critical for maintaining intact function. While, whether contributes to GCs PCOS, and ameliorates impairment through suppression...
Clusterization-triggered emission (CTE) from organic materials without π-conjugated structures for room temperature phosphorescence (RTP) is fascinating with extraordinary photophysical properties and diversified applications, but rather challenging in material design owing to the limited mechanism understanding. Here, we demonstrate a facile strategy construct CTE polymers stimuli-responsive emission, anti-Kasha RTP ultralong (OURTP) by introducing ions into hydrolyzed nonconjugated maleic...
Clinical use of antimicrobials faces great challenges from the emergence multidrug-resistant pathogens. The overexpression drug efflux pumps is one major contributors to multidrug resistance (MDR). Reversing function a promising approach overcome MDR. In life-threatening fungal pathogen Candida albicans, facilitator superfamily (MFS) transporter Mdr1p can excrete many structurally unrelated antifungals, leading Here we report counterintuitive case reversing MDR in C. albicans by using...
The insect chitinase OfChtI from the agricultural pest Ostrinia furnacalis (Asian corn borer) is a promising target for green insecticide design. critical chitinolytic enzyme cuticular chitin degradation at stage of molting. In this study, piperine, natural amide compound isolated black pepper, Piper nigrum L., was discovered first time to have inhibitory activity toward OfChtI. compound–enzyme interaction presumed take place between piperine benzo[d][1,3] dioxole skeleton and subsite −1...
The Rh(III)-catalyzed amidation of C(sp2)–H bonds has been reported by employing the N-methoxyamide as a novel amino source. An excellent level functional group tolerance can be achieved when derivatives are used amidating reagents. Importantly, several known bioactive compounds such Aminalon, Pregabalin, Gabapentin, and Probenecid transformed to effective reagents, way facilitate development new molecules.
Insect pest chitinases are potential target for developing new insect growth regulators. Piperine was found first to inhibit the chitinase (OfChi-h) from Ostrinia furnacalis (Asian corn borer) in this work, except previously reported OfChtI. Novel piperonyl-tethered rhodanine derivatives 7a-j were rationally designed with piperine as lead and synthesized by introducing a unique moiety into scaffold based on similar binding cavity of OfChtI OfChi-h. Compared piperine, compounds showed...
Abstract Many glycine-rich RNA-binding proteins (GR-RBPs) have critical functions in RNA processing and metabolism. Here, we describe a role for the tomato (Solanum lycopersicum) GR-RBP SlRBP1 regulating mRNA translation. We found that knockdown mutants (slrbp1) displayed reduced accumulation of total chlorophyll impaired chloroplast ultrastructure. These phenotypes were accompanied by deregulation levels numerous key transcripts associated with slrbp1. Furthermore, native...
Abstract Reducible oxides supported copper‐based catalysts have been widely used in ester hydrogenations due to their excellent catalytic performance. However, the role of surface oxygen vacancies is still unclear. Here, we fabricated four using different shaped CeO 2 nanocrystals as supports for hydrogenation methyl acetate (MA) ethanol. The activities significantly changed depending on morphology order rod>cube>spindle>octahedron, which was line with trend formation energy...
The application of some reported inhibitors against the chitinolytic enzyme Of ChtI was limited due to their unsatisfactory insecticidal activities. Hence, we first performed a synergetic design strategy combining π-stacking effect with aqueous solubility find novel rhodanine analogues inhibitory activities ChtI. Novel compounds IAa–f and IBa–f have weak solubility, but they (IAd: Ki = 4.0 μM; IBd: 2.2 μM) showed better comparable efficiency toward Ostrinia furnacalis compared high IIAa–f...
Adaptive response to physiological oxygen levels (physO2; 5% O2) enables embryonic survival in a low-oxygen developmental environment. However, the mechanism underlying role of physO2 supporting preimplantation development, remains elusive. Here, we systematically studied responses hallmark events development. Focusing on impeded transcriptional upregulation under atmospheric (atmosO2; 20% during 2-cell stage, functionally identified novel HIF-1α promoting major zygotic genome activation by...
Objective This experimental study investigated the protective function of quercetin on liver, spleen, and kidneys Goto-Kakizaki (GK) rats explores its mechanism action autophagy-related factors pathways. Materials methods GK were randomly divided into three groups: DM, DM + L-Que, H-Que, with age-matched Wistar serving as control group. The groups gavaged saline, quercetin-treated group was for 8 weeks each. Weekly blood glucose levels monitored. Upon conclusion experiment, samples gathered...