A5076255113- Gastrointestinal motility and disorders
- Memory and Neural Mechanisms
- Neuroscience and Neuropharmacology Research
- Congenital gastrointestinal and neural anomalies
- Infant Health and Development
- Nicotinic Acetylcholine Receptors Study
- Circadian rhythm and melatonin
- Neurobiology and Insect Physiology Research
- Neural dynamics and brain function
- Neuroendocrine regulation and behavior
- Gut microbiota and health
- Receptor Mechanisms and Signaling
- Helicobacter pylori-related gastroenterology studies
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Infant Nutrition and Health
- Advanced Fluorescence Microscopy Techniques
- Stress Responses and Cortisol
- Regulation of Appetite and Obesity
- Microscopic Colitis
- Sleep and related disorders
- Peroxisome Proliferator-Activated Receptors
- Cholinesterase and Neurodegenerative Diseases
- Sleep and Wakefulness Research
- Gene expression and cancer classification
National Institutes of Health
2020-2024
National Institute of Environmental Health Sciences
2021-2024
USF Health Byrd Alzheimer's Institute
2017
University of South Florida
2017
Morgan Stanley Children's Hospital
2011-2017
Columbia University Irving Medical Center
2017
Pediatrics and Genetics
2016
Columbia University
2011-2014
<h3>Objective</h3> Enterochromaffin cell-derived serotonin (5-HT) promotes intestinal inflammation. We tested hypotheses that peripheral tryptophan hydroxylase (TPH) inhibitors, administered orally, block 5-HT biosynthesis and deplete from enterochromaffin cells sufficiently to ameliorate inflammation; moreover, TPH inhibitors fail enter the murine enteric nervous system (ENS) or central systems thus do not affect constitutive gastrointestinal motility. <h3>Design</h3> Two LP-920540...
Autism spectrum disorder (ASD) is an increasingly common behavioral condition that frequently presents with gastrointestinal (GI) disturbances. It not clear, however, how gut dysfunction relates to core ASD features. Multiple, rare hyperfunctional coding variants of the serotonin (5-HT) transporter (SERT, encoded by SLC6A4) have been identified in ASD. Expression most SERT variant (Ala56) mice increases 5-HT clearance and causes ASD-like behaviors. Here, we demonstrated Ala56-expressing...
Disruption of normal circadian rhythm physiology is associated with neurodegenerative disease, which can lead to symptoms such as altered sleep cycles. In Alzheimer's disease (AD), dysfunction has been attributed β-amyloidosis. However, it unclear whether tauopathy, another AD-associated neuropathology, disrupt the clock. We have evaluated status clock in a mouse model tauopathy (Tg4510). Tg4510 mice display long free-running period at an age when present, and show evidence suprachiasmatic...
Necrotizing enterocolitis (NEC), a gastrointestinal inflammatory disease of unknown etiology that may also affect the liver, causes great deal morbidity and mortality in premature infants. We tested hypothesis signaling molecules, which are endogenous to bowel, regulate severity intestinal hepatic damage an established murine NEC model. Specifically, we postulated mucosal serotonin (5-HT), is proinflammatory, would exacerbate experimental oxytocin (OT), present enteric neurons...
SignificanceFiber photometry (FP) is a widely used technique in modern behavioral neuroscience, employing genetically encoded fluorescent sensors to monitor neural activity and neurotransmitter release awake-behaving animals. However, analyzing data can be both laborious time-consuming.AimWe propose the fiber analysis (FiPhA) app, which general-purpose FP application. The goal develop pipeline suitable for wide range of approaches, including spectrally resolved, camera-based, lock-in...
Cholinergic regulation of hippocampal theta oscillations has long been proposed to be a potential mechanism underlying hippocampus-dependent memory encoding processes. However, cholinergic transmission traditionally associated with type II under urethane anesthesia. The mechanisms and behavioral significance I in freely exploring animals is much less clear. In this study, we examined the object location task male mice that involves training testing trials provides an ideal study retrieval...
The activation of α7 nicotinic acetylcholine receptors (nAChRs) has been shown to improve hippocampus-dependent learning and memory. nAChRs are densely expressed among several different cell types in the hippocampus, with high Ca2+ permeability, although it is unclear if mobilize differential signalling mechanisms distinct neuronal populations. To address this question, we compared nAChR agonist-induced responses (i.e. calcium cAMP changes) between granule cells GABAergic neurons hippocampal...
Inclusion of male and female subjects in behavioral neuroscience research requires a concerted effort to characterize sex differences standardized assays. Sex hippocampus-dependent assays have been widely reported but are still poorly characterized. In the present study, we conducted parametric analysis spontaneous alternation, object recognition, fear conditioning commonly used control strain, C57BL/6NTac. Our findings show largely similar performance between males females across majority...
Adolescents naturally go to bed and awaken late, but are forced early for school work. This leads "social jetlag", a state of circadian desynchrony (CD), in which internal biological rhythms out sync with behavioral rhythms. CD is associated increased alcohol intake adults, has been less well-studied adolescents. The goal this study was model adolescent during similar conditions male C57BL/6J mice. Free access intake, water wheel-running activity were measured normal 12HR photoperiod or...
PGC1α is a transcriptional coactivator in peripheral tissues, but its function the brain remains poorly understood. Various brain-specific Pgc1α isoforms have been reported mice and humans, including two fusion transcripts (FTs) with non-coding repetitive sequences, their unknown. The FTs initiate at simple sequence repeat locus ∼570 Kb upstream from reference promoter; one also includes portion of short interspersed nuclear element (SINE). Using publicly available genomics data, here we...
Time-of-day effects have been noted in a wide variety of cognitive behavioral tests, and perturbation the circadian system, either at level master clock SCN or downstream, impairs hippocampus-dependent learning memory. A number kinases, including serine-threonine casein kinase 1 (CK1) isoforms CK1δ/ε, regulate timing period through post-translational modification proteins. Modulation these kinases presents novel treatment direction for deficits modulation. Here, we tested potential...
Object recognition tasks are widely used assays for studying learning and memory in rodents. typically involves familiarizing mice with a set of objects then presenting novel object or displacing an to location context. Learning inferred by relative increase time investigating the novel/displaced object. These widespread use, but there many inconsistencies way they conducted across labs. Two major contributors this lack consistency method measuring investigation standardization that used....
Abstract The peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1α) is known as a transcriptional in peripheral tissues but its function the brain remains poorly understood. Various brain-specific Pgc1α isoforms have been reported mice and humans, including transcripts derived from novel promoter about ∼580 Kb upstream reference gene. These incorporate repetitive sequences simple sequence repeat (SSR) short interspersed nuclear element (SINE) classes are predicted to...