It is now accepted that bone strength a complex property determined mainly by three factors: quantity, quality and turnover of the itself. Most patients who experience fractures due to fragility could never develop affectations related mass density (i.e. osteoporosis). In this work, effect secondary are analyzed simulating degradation one or more principal components (organic inorganic) while they inspected with nondestructive optical technique. From results obtained, strong correlation...

Abstract The polarization of macrophages M0 to M1 or M2 using molecules embedded in matrices and hydrogels is an active field study. design biomaterials capable promoting has become a paramount need nowadays, since the healing process modulate inflammatory response. In this work, several immunocytochemistry ELISA tests strongly suggest achievement collagen‐based membranes crosslinked with tri‐functionalized oligourethanes coated silica. Measuring amount TGF‐β1 secreted culture media by...

Abstract We describe a novel holographic otoscope system for measuring nanodisplacements of objects subjected to dynamic excitation. Such measurements are necessary quantify the mechanical deformation surfaces in mechanics, acoustics, electronics, biology, and many other fields. In particular, we interested sound‐induced motion biological samples, such as an eardrum. Our consists laser illumination delivery (IS), optical head (OH), image processing computer (IP) systems. The IS delivers...

In this work, we show a windowed phase-unwrapping technique that uses first-order dynamic system and scans the phase following its iso-phase contours. previous works, have shown low-pass systems are very robust useful in problems. However, it is well known all methods minimum signal-to-noise ratio they tolerate. This paper shows scanning within local windows using path strategy, unwrapping method increases tolerance to noise. way, improved approach, can unwrap maps where basic fails. Tests...

Digital holographic microscopy (DHM) is a technique that has high potential for analyzing biological samples and been successfully applied to the study of cells cell lines providing information about important parameters such as refractive index, morphology, dry mass, among others; it also found applicability effects therapeutic treatments. Finding size shape since they tend change in presence some pathologies. In this research work, we obtain morphology thickness index A375 melanoma line...

Dennis Gabor's seminal idea of a simple all-optical setup aimed at reconstructing the object wavefront stored on photographic plate gave birth little over seven decades ago to field holography. In 1971 Gabor obtained Nobel Prize in Physics for this invention. Still, road early days after his two first papers subject was one full obstacles, so scientific and engineering contemporaries put rest more than 10 years, until invention laser. This fact made holographic concept take off new...

Silicon nanoparticles (SiNps) are being explored as biomaterials in the design of fluorescent medical probes and immunomodulatory strategies. However, most synthesis methods reported literature to obtain SiNps show a complicated process, resulting dispersed solvents that hardly compatible with biological applications. This work reports simple methodology synthesize water. Some physicochemical properties such size, structure, composition, fluorescence evaluated. Furthermore, vitro...

Recently an optoelectronic holography system was deployed in the clinic with purpose of quantifying tympanic membrane (TM) displacements various mammal species, objective being understanding their middle ear biomechanics. The has in-line configuration where data gathered is decoded using lensless digital Fresnel approximation. This paper presents quantitative obtained from acoustically excited postmortem chinchilla's TM. To achieve this we used a robust customized windowed unwrapping method...

Abstract Damaged complex modular organs repair is a current clinical challenge in which one of the primary goals to keep their biological response. An interesting case study it porcine esophagus since tubular muscular tissue selected as raw material for engineering. The design esophageal constructs can draw on properties processed homologous extracellular matrix (ECM). In this work, we report decellularization multilayered from 1‐, 21‐ and 45‐days old piglets through combination reversible...

Immunomodulatory biomaterials have emerged as a promising approach to engineer wound healing. To achieve this task, the bioactivity of and an easy application are two key desirable characteristics. This work reports injectable gel system containing immune cells primed for By combining self-assembly type I collagen, cross-linked with trifunctional oligourethanes, silica particle entrapment, structured collagen network acts delivery vehicle macrophages. primes macrophages anti-inflammatory...

Suitable extraction of an object's wave field in off-axis digital holographic array is the core for any numerical reconstruction process. In this manuscript, we propose algorithm that reduces number pixels needed trimming, reducing notably its processing time without significant changes quality reconstructed image. Our method generates window containing a complete field. The energy generated must contain analyzed, defined, and used as reference value. proposal significantly operations...

Secretion of signaling molecules by macrophages is induced silica particles deposited onto decellularized tissue derived biomaterials.

The stability and bioactivity of biologic implants rely mainly on the control crosslinking process collagen. However, most common methods have no degree producing it excessively. This study outlines role collagen-based with oligourethane host response following reconstruction a rat full-thickness abdominal wall defect. We decellularized crosslinked bovine pericardial tissue to achieve two degrees. For implants, named as non-crosslinked (N-CL), collagen-amines were 0.42 ± 0.02 mmol/mg....

We use a digital holographic interferometric setup to assess, as proof of concept, two state-of-the-art sensors (CMOS and sCMOS cameras) that are widely used in nondestructive testing (NDT). This study is intended evaluate the image quality recorded by any camera NDT. The assessing relies on quantification optical phase information recovered cameras for this study. For this, we calculate signal-to-noise ratio, correlation coefficient, index (Q-index) main figures-of-merit. As far know,...

Second harmonic generation (SHG) microscopy is applied to image honey sugar crystals, with the advantage of avoiding surrounding signal liquid honey. It a non-contact method where specialized preparation not required. Since crystallization process depends upon its floral origin, among other conditions, analyzing crystallographic behavior results in challenging task as crystals are mixed and overlapped. This work introduces simple, multi-variable data analysis requiring only one SHG image,...

Repair of damaged or faulty complex modular organs such as the urinary bladder is a current clinical challenge. The design constructs for reconstructive urological surgery can draw advantage from bioactivity natural extracellular matrix (ECM) bioscaffolds, well activity provided by cells seeded into constructs. Considering these benefits, this work compares performance pericardial ECM bioscaffolds and with gel-supported urothelial in repair defects rabbits. considered study are porcine (pM)...

Recently, we introduced a Digital Optoelectronic Holographic System (DOEHS) for measurement of acoustically induced deformations the human tympanic membrane (TM) in order to study and diagnose pathologic conditions middle-ear. The DOEHS consists laser-delivery illumination (IS), optical head (OH), image-processing computer (IP), positioning arm (PS) subsystems. information is recorded by CCD numerically reconstructed Fresnel approximation. Our holographic otoscope system currently deployed...

The simultaneous surface and internal measurements from a chemically modified cortical bovine bone suffering plastic range deformation are presented. Since the is an anisotropic structure, its mechanical response could be if organic or inorganic phases change. latter result in high deformations, where interferometrical signal optical analysis easily de-correlated. In this work, digital holography interferometry (DHI) Fourier domain coherence tomography (FD-OCT) used to analyze of under...

A study in porcine femoral bones with and without the presence of cortical drilling is presented. An out plane digital holographic interferometer used to retrieve optical phase during controlled compression tests. These tests try simulate physiological deformations postmortem healthy compare their mechanical response those having a hole. The technique widely medical procedures fix plaques metallic frames bone recovering from fracture. Several materials techniques are for this purpose. In...

Bee honey is an exceptionally nutritious food with unique chemical and mineral contents. This report introduces the use of second-harmonic generation (SHG) microscopy for imaging sugar crystals’ morphology as alternative its authentication process. The crystals their boundaries are clearly observed SHG compared bright-field microscopy, where liquid avoids visualization a sharp image. Four different samples Mexico’s various floral origins geographical regions analyzed in our study. These...

In this paper, we introduce a positioning system to self-adjust the spectral components of holograms recorded by an off-axis digital holography setup. The incidence angle reference arm is carried out two motorized actuators controlled algorithm that automatically adjusts it avoid overlapping components. right allows selection region extract only virtual component so that, after inverse Fourier transformation, object wave field can be obtained, thus eliminating undesired and increasing image...