A5101982750- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Insect Resistance and Genetics
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Insect symbiosis and bacterial influences
- Animal Virus Infections Studies
- Mosquito-borne diseases and control
- Protein purification and stability
- Cancer Research and Treatments
- Insect and Pesticide Research
- 3D Printing in Biomedical Research
- Entomopathogenic Microorganisms in Pest Control
- Spine and Intervertebral Disc Pathology
- Viral Infections and Immunology Research
- Veterinary Orthopedics and Neurology
- Bacteriophages and microbial interactions
- Invertebrate Immune Response Mechanisms
- Hepatitis C virus research
- Ubiquitin and proteasome pathways
- Viral Infections and Vectors
- Plant and Fungal Interactions Research
- Toxoplasma gondii Research Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
Yangzhou University
2012-2020
Chinese Academy of Sciences
2003-2012
Wuhan Institute of Virology
2003-2012
Wageningen University & Research
2012
Central South University
2006
University of Chinese Academy of Sciences
2005
ABSTRACT Autographa californica multiple nucleopolyhedrovirus (AcMNPV) BV/ODV-c42 ( orf101 ; c42 ), which encodes a 41.5-kDa viral nucleocapsid protein with putative nuclear localization signal (NLS) motif at the C terminus, is highly conserved gene among members of Baculoviridae family. C42 demonstrated to be essential for AcMNPV propagation and can bind P78/83, activator actin-related 2/3 (ARP2/3) complex initiate actin polymerization, morphogenesis during infection. Here, we report...
A recombinant baculovirus vector expressing GFP (Ac-CMV-GFP) was tested in rabbit intervertebral disc cells cultured monolayer vitro. Direct infection of then assessed vivo.To test the efficacy baculovirus-mediated marked gene transfer to cells, and expression transgene vitro vivo.The potential application therapy for treatment degenerative disease has been an area exciting active research. Many efforts have made toward construction delivery vectors using mammalian viruses, such as...
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) belonging to the group I nucleopolyhedroviruses (NPVs) and expressing envelope-fusion glycoprotein GP64 transduces a variety of mammalian cells express foreign genes under control promoters. In contrast, II Helicoverpa armigera single NPV (HaSNPV) encoding different envelope protein, F shows no detectable infectivity towards cells. This limitation was overcome by AcMNPV in HaSNPV. Although transduction ratios were lower overall,...
The HA2 protein of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearNPV) is a WASP homology capable nucleating branched actin filaments in presence Arp2/3 complex vitro. To determine role ha2 HearNPV life cycle, knockout and repair bacmids were constructed. Transfection infection analysis demonstrated that null bacmid was unable to produce infectious budded virus (BV), while rescued defect. In vitro WCA domain accelerates Arp2/3-mediated assembly indispensable function...