A5020397703- Microfluidic and Capillary Electrophoresis Applications
- Advanced Biosensing Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Microfluidic and Bio-sensing Technologies
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- Analytical Chemistry and Chromatography
- SARS-CoV-2 and COVID-19 Research
- Plant-based Medicinal Research
- Birth, Development, and Health
- Cardiovascular Function and Risk Factors
- Biosensors and Analytical Detection
- Cannabis and Cannabinoid Research
- CNS Lymphoma Diagnosis and Treatment
- Advanced Chemical Sensor Technologies
- Multiple Myeloma Research and Treatments
- Protein purification and stability
- Hemoglobinopathies and Related Disorders
- Hemoglobin structure and function
- Congenital Heart Disease Studies
- Alkaloids: synthesis and pharmacology
- Pregnancy and preeclampsia studies
- Forensic Toxicology and Drug Analysis
Stanford University
2006-2025
Stanford Health Care
2022-2025
Guizhou Provincial People's Hospital
2023
Palo Alto University
2022
San Francisco General Hospital
2019-2021
University of California, San Francisco
2019-2021
Peking University
2002-2019
We have designed and fabricated a polydimethylsiloxane (PDMS) microfluidic device containing an array of gold spots onto which antigens or antibodies interest can be attached. use surface plasmon resonance (SPR) imaging to monitor the antibody-antigen recognition binding events. This combination offers two significant advantages: (1) dramatically reduces reaction time sample consumption; (2) SPR yields real-time detection immunocomplex formation. Thus, immunoreaction may detected...
The kinetics of IgG avidity maturation during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection was studied. assay, using a novel label-free immunoassay technology, revealed strong correlation between and days since symptom onset. Peak readings were significantly higher in than mild disease cases.
Methods designed to measure severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) humoral response include virus neutralization tests determine antibody activity. For ease of use and universal applicability, surrogate (sVNTs) based on antibody-mediated blockage molecular interactions have been proposed. A test was established a label-free immunoassay platform (LF-sVNT). The LF-sVNT analyzes the binding ability SARS-CoV-2 spike protein receptor-binding domain (RBD)...
Identification of hemoglobin (Hb) variants is significant value in the clinical diagnosis hemoglobinopathy. However, conventional methods for identification Hb laboratories can be inadequate due to lack structural characterization. We describe use neutral-coating capillary electrophoresis coupled with high-resolution mass spectrometry (CE-HR-MS) achieve high-performance top-down variants.An Orbitrap Q-Exactive Plus spectrometer was an ECE-001 (CE) unit through EMASS-II ion source. A PS1 used...
Background: Markers of aging hold promise in the context colorectal cancer (CRC) care.Utilizing high-resolution metabolomic profiling, we can unveil distinctive age-related patterns that have potential to predict early CRC development.Our study aims unearth a panel markers and delve into alterations associated with CRC.Methods: We assembled serum cohort comprising 5,649 individuals, consisting 3,002 healthy volunteers, 715 patients diagnosed advanced precancerous lesions (APL), 1,932...
A procedure is described for making layer-to-layer interconnections in polydimethylsiloxane (PDMS) microfluidic devices. Thin (∼50 μm) perforated PDMS membranes are bonded to thicker (0.1 cm or more) slabs by means of thermally cured prepolymer form a three-dimensional (3D) channel structure, which may contain valve arrays that can pass over and under one another. Devices containing as many two three demonstrated. We also present 3D devices display liquid dispensing.
Cannabis use results in impaired driving and an increased risk of motor vehicle crashes. Cannabinoid concentrations blood other matrices can remain high long after use, prohibiting the differentiation between acute chronic exposure. Exhaled breath has been proposed as alternative matrix which may more closely correspond to window impairment; however, efficient capture analytically sensitive detection methods are required for measurement.Timed samples were collected from 20 volunteers before...
Abstract Background Immunoassays based on label-free technologies (label-free immunoassay [LFIA]) offer an innovative approach to clinical diagnostics and demonstrate great promise for therapeutic drug monitoring (TDM) of monoclonal antibody (mAb) drugs. An LFIA measures immunocomplex formation in real time allows quantification initial binding rate, which facilitates fast measurement within a few minutes. Methods Based thin-film interferometry (TFI) technology, open-access LFIAs were...
The electroosmotic flow (EOF) in a poly(dimethylsiloxane) (PDMS) separation channel can be altered and controlled by adding carboxylic acid to the prepolymer prior curing. When is doped with 0.5 wt % undecylenic (UDA), mobility modified PDMS rises (7.6 ± 0.2) × 10-4 cm2 V-1 s-1 (in HEPES buffer at pH 8.5), which nearly twice that native channel. Because this modification does not significantly change hydrophobicity of surface, it possible combine dynamic coating n-dodecyl β-d-maltoside...
As the legalization of medical and recreational marijuana use expands, measurement tetrahydrocannabinol (THC) in human breath has become an area interest. The presence concentration cannabinoids have been shown to correlate with recent may be correlated impairment. Given low THC breath, sensitive analytical methods are required further evaluate its utility window detection. This paper describes a novel derivatization method based on azo coupling reaction that significantly increases...
Abstract The kinetics of IgG avidity maturation during SARS-CoV-2 infection was studied. assay used a novel label-free immunoassay technology. It found that there strong correlation between and days since symptom onset, peak readings were significantly higher in severe than mild disease cases.
Preeclampsia (PE) is a condition that poses significant risk of maternal mortality and multiple organ failure during pregnancy. Early prediction PE can enable timely surveillance interventions, such as low-dose aspirin administration. In this study, conducted at Stanford Health Care, we examined cohort 60 pregnant women collected 478 urine samples between gestational weeks 8 20 for comprehensive metabolomic profiling. By employing liquid chromatography mass spectrometry (LCMS/MS), identified...
Due to the low concentration of androgens in women and limitation immunoassays, it remains a challenge accurately determine levels serum polycystic ovary syndrome (PCOS) patients for clinical laboratories. In this report, liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed validated simultaneous quantitation testosterone (T), androstenedione (A4), dehydroepiandrosterone sulfate (DHEAS), dihydrotestosterone (DHT), 17-hydroxyprogesterone (17-OHP) that are associated...
Affinity capture of an analyte by a agent is one the most effective sample preparation approaches in mass spectrometry (MS), especially top-down MS. We describe new affinity technique for protein targets, called microprobe-capture in-emitter elution (MPIE), which can directly couple label-free optical sensing technology (next-generation biolayer interferometry, BLI) with To implement MPIE, first captured on surface microprobe and subsequently eluted from inside electrospray emitter. The...
Therapeutic drug monitoring (TDM) of immunosuppressants is essential for optimal care transplant patients. Immunoassays and liquid chromatography-mass spectrometry (LC-MS) are the most commonly used methods TDM. However, immunoassays can suffer from interference heterophile antibodies structurally similar drugs metabolites. Additionally, nominal-mass LC-MS assays be difficult to optimize limited in number detectable compounds.The aim this study was implement a mass spectrometry-based test...
Abstract Cerebrospinal fluid (CSF) leak can be diagnosed in clinical laboratories by detecting a diagnostic marker β 2 -transferrin (β -Tf) secretion samples. -Tf and the typical transferrin (Tf) proteoform serum, 1 -Tf), are Tf glycoforms. An innovative affinity capture technique for sample preparation, called microprobe-capture in-emitter elution (MPIE), was incorporated with high-resolution mass spectrometry (HR-MS) to study glycoforms primary structures of -Tf. To implement MPIE, an...
Abstract Objective Primary aldosteronism (PA), a significant cause of secondary hypertension affecting ∼10% patients with severe hypertension, exacerbates cardiovascular, and cerebrovascular complications even after blood pressure control. PA is categorized into two main subtypes: unilateral aldosterone-producing adenomas (APA) bilateral hyperaldosteronism (BHA), each requiring distinct treatment approaches. Accurate subtype classification crucial for selecting the most effective treatment....
Abstract How people process gaze cues from multiple others is an important topic but rarely studied. Our study investigated this question using adapted cueing paradigm to examine the effect of gazes and its neural correlates. We manipulated directions two human avatars be either convergent, created by simultaneously averting their same direction, or non-convergent, when only one shifted gaze. results showed faster reaction times larger target-congruency effects following convergent shared...
Reliable biomarkers are needed to improve preeclampsia (PE) prediction accuracy. With the investigational tool of peptidomics, we aimed identify and validate potential serum peptide in cohorts suspected for PE development middle or late pregnancy.Totally 195 samples were prospectively collected from pregnant women with PE-related syndromes who followed up until delivery. Serum peptidomic analysis was performed discovery cohort 115 using matrix-assisted laser desorption ionization-time flight...
Many natural products have biological effects on humans and animals. Poisoning caused by is common in clinical toxicology cases. Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) has recently emerged as a powerful analytical tool for large-scale target screening, the application of LC-HRMS can be expanded to evaluate potential product poisoning We report construction an spectral library 95 commonly implicated poisoning, assay was validated definitive detection urine serum...
Liquid chromatography-high-resolution mass spectrometry (LC-HR-MS) has emerged as a powerful analytical technology for compound screening in clinical toxicology. To evaluate the potential of LC-HR-MS3 detecting toxic natural products, spectral library 85 products (79 alkaloids) that contains both MS2 and MS3 spectra was constructed used to identify products. Samples were analyzed using an method generated data matched test performance different sample matrices, product standards divided into...