A5053931189- SARS-CoV-2 and COVID-19 Research
- Forensic Toxicology and Drug Analysis
- COVID-19 Clinical Research Studies
- Quantum Chromodynamics and Particle Interactions
- Particle physics theoretical and experimental studies
- Poisoning and overdose treatments
- High-Energy Particle Collisions Research
- Opioid Use Disorder Treatment
- SARS-CoV-2 detection and testing
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Cannabis and Cannabinoid Research
- COVID-19 Impact on Reproduction
- Analytical Chemistry and Chromatography
- Long-Term Effects of COVID-19
- Monoclonal and Polyclonal Antibodies Research
- Neutrino Physics Research
- Hormonal and reproductive studies
- Veterinary Pharmacology and Anesthesia
- Respiratory viral infections research
- Acute Myocardial Infarction Research
- Neurotransmitter Receptor Influence on Behavior
- Advanced Biosensing Techniques and Applications
- Pharmaceutical studies and practices
- Cellular transport and secretion
University College Dublin
2023-2025
University of California, San Francisco
2015-2024
University of California San Francisco Medical Center
2024
Universitat Ramon Llull
2023
San Francisco General Hospital
2014-2023
RWTH Aachen University
2023
Chan Zuckerberg Initiative (United States)
2021-2022
Lifespan
2021
Weatherford College
2021
University of California System
2021
ABSTRACT Background Serological tests are crucial tools for assessments of SARS-CoV-2 exposure, infection and potential immunity. Their appropriate use interpretation require accurate assay performance data. Method We conducted an evaluation 10 lateral flow assays (LFAs) two ELISAs to detect anti-SARS-CoV-2 antibodies. The specimen set comprised 128 plasma or serum samples from 79 symptomatic RT-PCR-positive individuals; 108 pre-COVID-19 negative controls; 52 recent individuals who underwent...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can be detected indirectly by measuring the host immune response. For some viruses, antibody concentrations correlate with protection and viral neutralization, but in rare cases, antiviral antibodies promote disease progression. Elucidation of kinetics magnitude SARS-CoV-2 response is essential to understand pathogenesis 2019 (COVID-19) identify potential therapeutic targets.Sera (n = 533) from patients real-time...
Neutralizing autoantibodies against type I interferons (IFNs) have been found in some patients with critical coronavirus disease 2019 (COVID-19), the caused by severe acute respiratory syndrome 2 (SARS-CoV-2). However, prevalence of these antibodies, their longitudinal dynamics across severity scale, and functional effects on circulating leukocytes remain unknown. Here, 284 COVID-19, we IFN–specific peripheral blood samples from 19% 6% disease. We no IFN individuals moderate Longitudinal...
Abstract Studies have demonstrated that at least 20% of individuals infected with SARS-CoV-2 remain asymptomatic 1–4 . Although most global efforts focused on severe illness in COVID-19, examining infection provides a unique opportunity to consider early immunological features promote rapid viral clearance. Here, postulating variation the human leukocyte antigen ( HLA ) loci may underly processes mediating infection, we enrolled 29,947 individuals, for whom high-resolution genotyping data...
Abstract Long COVID (LC) occurs after at least 10% of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, yet its etiology remains poorly understood. We used ‘omic” assays and serology to deeply characterize the global SARS-CoV-2-specific immunity in blood individuals with clear LC non-LC clinical trajectories, 8 months postinfection. found that exhibited systemic inflammation immune dysregulation. This was evidenced by differences T cell subset distribution implying...
BackgroundMost cohorts show similar or lower COVID-19 incidence among people living with HIV compared the general population. However, might be affected by testing rates vulnerable populations. We aimed to compare SARS-CoV-2 IgG seroprevalence, disease severity, and neutralising antibody activity after infection without receiving care in a county hospital system over 3-month period.MethodsIn this matched case-control observational study, remnant serum samples were collected between Aug 1 Oct...
Long COVID (LC), a type of post-acute sequelae SARS-CoV-2 infection (PASC), occurs after at least 10% infections, yet its etiology remains poorly understood. Here, we used multiple "omics" assays (CyTOF, RNAseq/scRNAseq, Olink) and serology to deeply characterize both global SARS-CoV-2-specific immunity from blood individuals with clear LC non-LC clinical trajectories, 8 months following prior receipt any vaccine. Our analysis focused on deep phenotyping T cells, which play important roles...
Until recently, there existed minimal guidance on the use of LC-MS for clinical diagnostics. The Clinical and Laboratory Standards Institute (CLSI)2 has now presented a standardized approach assay development verification in its new document, CLSI C62-A. This document should aid harmonization methods thus have significant ramifications evolution this technology laboratory. ability to accurately identify quantify measurand with high sensitivity specificity by selective reaction monitoring...
the California Poison Control System-San Francisco division identified 8 patients who experienced adverse effects associated with ingestion of counterfeit alprazolam tablets found to contain fentanyl and, in some cases, etizolam.The identification these resulted a coordinated response that included state and local public health departments, toxicology laboratory, media outlets, an investigation by law enforcement agencies.
On the evening of June 23, 2016, a white powder advertised as cocaine was purchased off streets from multiple sources and used by an unknown number persons in New Haven, Connecticut. During period less than 8 hours, 12 patients were brought to emergency department (ED) at Yale Haven Hospital, experiencing signs symptoms consistent with opioid overdose. The route intoxication not known, but presumed be insufflation ("snorting") most cases. Some required doses antidote naloxone exceeding 4 mg...
Although T cells are likely players in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunity, little is known about the phenotypic features of SARS-CoV-2-specific associated with recovery from disease 2019 (COVID-19). We analyze 34 individuals COVID-19 severity ranging mild (outpatient) to critical, culminating death. Relative who succumbed, recovered harbor elevated and increasing numbers capable homeostatic proliferation. In contrast, fatal cases display regulatory a...
Abstract Background SARS-CoV-2 infection can be detected indirectly by measuring the host immune response. Anti-viral antibody concentrations generally correlate with protection and viral neutralization, but in rare cases, antibodies promote disease progression. Elucidation of kinetics magnitude response is essential to understand pathogenesis COVID-19 identify potential therapeutic targets. Methods Sera (n=533) from patients RT-PCR confirmed (n=153) were tested using a high-throughput...
Limited data are available on the long-term clinical and immunologic consequences of SARS-CoV-2 infection in people with HIV (PWH).We measured SARS-CoV-2-specific humoral cellular responses without recovering from COVID-19 ( n = 39 43, respectively) using binding antibody, surrogate virus neutralization, intracellular cytokine staining, inflammatory marker assays. We identified individuals experiencing postacute sequelae (PASC) evaluated parameters. used linear regression generalized models...
Studies are needed to evaluate the safety and effectiveness of mRNA SARS-CoV-2 vaccination during pregnancy, levels protection provided their newborns through placental transfer antibodies. Here, we transplacental vaccine products functional anti-SARS-CoV-2 antibodies pregnancy early infancy in a cohort 20 individuals vaccinated late pregnancy. We find no evidence maternal blood, placenta tissue, or cord blood at delivery. However, time-dependent efficient IgG neutralizing neonate that...
Abstract Background The overdose crisis driven by synthetic opioids continues to escalate in the USA. We evaluated efficacy of multiple manufacturing lots a fentanyl test strip (FTS) detect and analogs assessed cross-reactivity with possible interferences. Methods Drug standards were dissolved water laboratory setting serially diluted. dilutions tested using five different BTNX Rapid Response (20 ng/mL cutoff) lateral flow chromatographic immunoassay strips assess lot-to-lot variability for...
In regulated vesicle exocytosis, SNARE protein complexes drive membrane fusion to connect the lumen with extracellular space. The triggering of pore formation by Ca(2+) is mediated specific isoforms synaptotagmin (Syt), which employ both complex and binding. also promotes expansion Syts have been implicated in this process but mechanisms involved are unclear. We determined role Ca(2+)-dependent Syt-effector interactions expressing Syt-1 mutants selectively altered binding or insertion PC12...
Liquid chromatography high-resolution mass spectrometry (LC-HRMS) with untargeted data collection is especially attractive for general unknown drug screening owing to its ability identify unexpected compounds. LC-HRMS offers several advantages over traditional selected reaction monitoring (SRM) techniques and could be an ideal platform as long analytical performance comparable that of SRM-based methods. We developed a broad-spectrum screen on spectrometer [tandem quadrupole time-of-flight...
The kinetics of IgG avidity maturation during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection was studied. assay, using a novel label-free immunoassay technology, revealed strong correlation between and days since symptom onset. Peak readings were significantly higher in than mild disease cases.
Abstract Background Laboratory tests that use streptavidin–biotin binding mechanisms have the potential to be affected by high circulating biotin concentrations, which would produce positive and negative interference in biotinylated competitive noncompetitive (sandwich) immunoassays, respectively. Consumption of high-dose supplements for cosmetic or health-related reasons has drawn attention clinical laboratory tests. Case reports vivo studies show ingestion supplemental can cause clinically...
Biomarkers have been widely explored for coronavirus disease 2019 diagnosis. Both viral RNA or antigens (Ag) in the respiratory system and antibodies (Ab) blood are used to identify active infection, transmission risk, immune response but limitations. This study investigated diagnostic utility of severe acute syndrome 2 (SARS-CoV-2) nucleocapsid protein (N-Ag) serum.We retrospectively studied 208 randomly selected cases with SARS-CoV-2 infection confirmed by test swabs. N-Ag concentrations...