A5066772857- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Psoriasis: Treatment and Pathogenesis
- MicroRNA in disease regulation
- Pluripotent Stem Cells Research
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- Calcium signaling and nucleotide metabolism
- Uterine Myomas and Treatments
- Wound Healing and Treatments
- Breast Implant and Reconstruction
- Endometriosis Research and Treatment
- Dermatology and Skin Diseases
- 3D Printing in Biomedical Research
- Cytokine Signaling Pathways and Interactions
- Telomeres, Telomerase, and Senescence
- Neurogenesis and neuroplasticity mechanisms
- Sperm and Testicular Function
- Electrospun Nanofibers in Biomedical Applications
- Neuroscience and Neuropharmacology Research
- Immune cells in cancer
- Lipid Membrane Structure and Behavior
- Dental Implant Techniques and Outcomes
- Extracellular vesicles in disease
Marche Polytechnic University
2015-2025
European School of Molecular Medicine
2023
Zero to Three
2013
The spontaneous expression of neural markers, already demonstrated in bone marrow (BM) mesenchymal stem cells (MSCs), has been considered as evidence the MSCs’ predisposition to differentiate toward lineages, supporting their use cell-based therapy for repair. In this study we have evaluated, by immunocytochemistry, immunoblotting, and flow cytometry experiments, markers undifferentiated MSCs from different sources: human adipose (hASCs), skin-derived (hS-MSCs), periodontal ligament...
Although the etiology of leiomyoma is unclear, a progenitor/undifferentiated cell population has been described whose dysregulation may be involved in onset uterine conditions. Moreover, inflammation development several tumors. The aim this work was to understand if progenitor cells sustain chronic inflammatory microenvironment that enhances development. Cells from 12 human and normal myometrium samples same patients were vitro isolated exhaustively characterized (morphology, proliferation,...
The expression of inducible nitric oxide synthase (iNOS) and vascular endothelial growth factor (VEGF) the level total oxyradical scavenging capacity have been evaluated extensively in cutaneous cells patients with psoriasis. As yet, no indications are available about undifferentiated cells, mesenchymal stem (MSCs), isolated from skin.To isolate MSCs psoriasis to compare them those obtained atopic healthy subjects, order analyse whether show some typical psoriatic profiles understand...
Psoriasis is a Th1 immune-mediated, inflammatory disease, in which skin lesions appear many years before the related metabolic and cardiovascular comorbidities, according to theory of 'psoriatic march'. Inducible nitric oxide synthetase (iNOS), tumour necrosis factor (TNF)-α vascular endothelial growth (VEGF) are directly implicated determining both systemic involvement psoriasis. Reactive oxygen species actively promote secretion cytokines involved pathogenesis psoriasis.Evaluation VEGF...
Atopic dermatitis (AD) is a chronic and inflammatory disease characterized by marked imbalance of T helper (Th)2 vs. Th1/Th17 cells in the early phase AD, whereas mixed Th1/Th2 pattern inflammation usually found at stage. These features have not been extensively evaluated undifferentiated skin patients affected AD.To evaluate relative expression 22 genes encoding Th1, Th2 Th17 cytokines secretion corresponding proteins cutaneous mesenchymal stem (MSCs) isolated from with AD (AD-MSCs) their...
Human dental pulp is considered an interesting source of adult stem cells, due to the low-invasive isolation procedures, high content cells and its peculiar embryological origin from neural crest. Based on our previous findings, a sub-population, enriched for expression STRO-1, c-Kit CD34, showed higher commitment. However, their biological properties were compromised when cultured in adherent standard conditions. The aim this study was evaluate ability three dimensional floating spheres...
Abstract Psoriasis is a disease characterized by an imbalance between Th 1 and 17 2 inflammatory axes, in which cutaneous mesenchymal stem cells ( MSC s) are early involved, as they show greater relative expression of several genes encoding for cytokines. Therapeutic implications TNF ‐α inhibitors on differentiated skin have been largely described psoriasis; however, their effects s derived from patients with psoriasis only partially described. The aim this work was to evaluate the effect...
Bone tissue engineering is a promising research area for the improvement of traditional bone grafting procedure drawbacks. Thanks to capability self-renewal and multi-lineage differentiation, stem cells are one major actors in approaches, adult mesenchymal (MSCs) considered be appropriate regenerative medicine strategies. marrow MSCs (BM-MSCs) earliest- discovered well-known cell population used engineering. However, several factors hamper BM-MSC clinical application subsequently, new...
CD38 is a type II glycoprotein that acts both as bifunctional enzyme, responsible for the synthesis and hydrolysis of cyclic ADP-ribose, signal-transducing surface receptor. Although was originally described plasma membrane molecule, several reports indicate expressed in nucleus, even cells known to be surface-negative. In this study, firstly we investigated presence nuclear by immunofluorescence confocal microscopy using panel hematopoietic cell lines exhibit different levels expression....
Bone marrow is a useful cell source for skeletal tissue engineering approaches. In vitro differentiation of mesenchymal stem cells (MSCs) to chondrocytes or osteoblasts can be induced by the addition specific growth factors medium. The present study evaluated behaviour human MSCs cultured on various scaffolds determine whether their cell-matrix interactions. from bone collected acetabulum during hip arthroplasty procedures were isolated sorting, expanded and characterised flow cytometry...
Psoriasis microenvironment, characterized by an imbalance between T helper type 1 (Th1)/Th17 and Th2 cytokines also influences the mesenchymal stem cells (MSCs) phenotypical profile. MSCs from healthy donors (H-MSCs) can exert a strong paracrine effect secreting active soluble factors, able to modulate inflammation in microenvironment. To evaluate influence of H-MSCs on psoriatic patients (PsO-MSCs), PsO-MSCs were isolated characterized. Indirect co-culture with was performed; effects...
Alzheimer’s disease (AD) is a widespread neurodegenerative disorder, affecting large number of elderly individuals worldwide. Mitochondrial dysfunction, metabolic alterations, and oxidative stress are regarded as cooperating drivers the progression AD. In particular, impairment amplifies production reactive oxygen species (ROS), resulting in detrimental alterations to intracellular Ca2+ regulatory processes. The Na+/Ca2+ exchanger (NCX) proteins key pathophysiological determinants Na+...