A5033211215- Syphilis Diagnosis and Treatment
- Reproductive tract infections research
- Sex work and related issues
- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Female Genital Mutilation/Cutting Issues
- COVID-19 Clinical Research Studies
- Reproductive System and Pregnancy
- Autoimmune Bullous Skin Diseases
- Cancer-related molecular mechanisms research
- Mycobacterium research and diagnosis
- Body Image and Dysmorphia Studies
- RNA Interference and Gene Delivery
- Blood Coagulation and Thrombosis Mechanisms
- HIV Research and Treatment
- Virology and Viral Diseases
- RNA modifications and cancer
- Biosensors and Analytical Detection
- HIV/AIDS Research and Interventions
- COVID-19 epidemiological studies
- HIV, Drug Use, Sexual Risk
- Parvovirus B19 Infection Studies
- Food Allergy and Anaphylaxis Research
- HIV, TB, and STIs Epidemiology
Zhongshan Hospital of Xiamen University
2016-2025
Xiamen University
2011-2025
Xiamen Medical College
2022-2025
Southwest Jiaotong University
2024
Anhui Provincial Children's Hospital
2023
Anhui Medical University
2023
Chongqing Jiaotong University
2023
China Railway Group (China)
2023
Health First
2023
Chongqing Medical University
2022
Background. Algorithms for the diagnosis of syphilis continue to be a source great controversy, and numerous test interpretations have perplexed many clinicians.
Background. The prozone phenomenon is known to be associated with high antibody titers; other associations, such as host factors, have not been elucidated.
Background Some syphilis patients remain in a serologically active state after the recommended therapy. We currently know too little about characteristics of this serological response. Methods conducted cohort study using clinical database from Zhongshan Hospital, Medical College Xiamen. In total, 1,327 HIV-negative with primary, secondary, latent, and tertiary were enrolled. Bivariate multivariate analyses utilised to identify factors associated cure serofast one year Chi-square tests used...
Due to anti-SARS-CoV-2 antibody decay and SARS-CoV-2 variants, vaccine booster doses are a constant concern. It was focused on whether the third dose can quickly evoke activate immunity produce sufficient durable immune protection.To evaluate responses durations of five subsets antibodies their predictive values for protection after administration three-dose inactivated vaccines regimens.A prospective cohort study (neutralizing antibody, anti-RBD total anti-Spike IgG, IgM, IgA) carried out...
Abstract Exosomes were enriched from plasma by ultracentrifugation, precipitation, and membrane-based approaches for yield purity. Using the four isolation approaches, particles with mode sizes within expected range (50–200 nm) can be isolated. By protein estimation, polymer precipitation resulted in a maximum (5610.59 ± 51.189 µg/mL), followed membrane affinity (471.57 12.16 ultracentrifugation (440.22 11.71 µg/mL) filter + (235.47 13.27 µg/mL). total RNA of (3.26 0.42 ng/mL) was higher...
The clinical spectrum of neurosyphilis (NS) has changed over time.To describe the and characteristics NS in HIV-negative patients.A retrospective chart review was performed for 149 patients with NS.All were >25 years old, including 16.8% asymptomatic NS, 15.4% syphilitic meningitis, 24.2% meningovascular 38.9% general paresis, 4.0% tabes dorsalis 0.7% gummatous NS. original misdiagnosis rate 84.6%. All had positive serum Treponema pallidum particle agglutination (TPPA) rapid plasma reagin...
Neurotrophic keratopathy (NK) is a degenerative corneal disease characterized by impaired sensitivity and epithelial repair that often linked to sensory nerve dysfunction. To establish clinically relevant model explore the mechanisms underlying NK pathogenesis, we developed novel mouse through partial transection of ciliary nerve. This approach mimics progressive nature NK, reproducing key clinical features such as defects, reduced sensitivity, diminished tear secretion, delayed wound...
Abstract Characterizing the immune response and pathogen tissue dissemination during progressive Treponema pallidum ( T. ) infection in human is crucial for comprehending syphilis but it remains poorly understood, due to unethical nature of inoculating volunteers with pallidum. An urgent new model needed study this issue. A haematopoietic stem cell (Hu‐HSC) mice was constructed through inoculation . Blood samples were collected at serial time points (0, 3, 7, 14, 28 42 days post‐infection)...
This study characterizes psychiatric manifestations as a primary symptom of neurosyphilis (NS). Fifty-two the 169 NS patients presented with manifestations, many had characteristics more than one syndrome, including cognitive impairment, personality disorders, delirium, hostility, dysarthria, confusion, disruption their sleep-wake cycle, fecal and urinary incontinence, dysphoria, paranoia, hallucinations, expansive mood, mania. positive sera RPR T. pallidum particle agglutination (TPPA), 75%...
The origin of nontreponemal antibodies during syphilis infection is hotly debated. Here, we analyzed the immune response in rabbits immunized with various antigens. Inactivated treponemes elicited production low-titer some rabbits. Cardiolipin combined bovine serum albumin also induced anticardiolipin antibody production. These findings indicate that Treponema pallidum contained a cardiolipin antigen weak immunogenicity. However, active T. higher strong immunogenicity at an earlier time...
Glycolysis is a critical pathway in cellular glucose metabolism that provides energy and participates immune responses. However, whether glycolysis involved NOD-like receptor family protein 3 (NLRP3) inflammasome activation phagocytosis of macrophages response to Treponema pallidum infection remains unclear.To investigate the role activating NLRP3 for regulating T. Tp47 its associated mechanisms.Interactions between Tp47-treated were investigated through experiments on peritoneal human...
Known predictors of neurosyphilis were mainly drawn from human immunodeficiency virus (HIV)-infected syphilis patients, which may not be applicable to HIV-negative populations as they have different characteristics, particularly those with neurological symptoms. This study aimed identify novel symptomatic (S-NS). From June 2005 2015, 370 patients symptoms recruited, consisting 191 S-NS (including 123 confirmed and 68 probable patients) 179 syphilis/non-neurosyphilis (N-NS) patients. Clinical...
This study aimed to determine whether a serological response could predict the normalization of cerebrospinal fluid (CSF) abnormalities at 6 months after treatment in human immunodeficiency virus (HIV)-negative neurosyphilis patients. A total 123 patients were recruited baseline, 58 these undergoing treatment, repeated CSF examinations and tests for syphilis included follow-up study. Before rapid plasma reagin (RPR) titer, Treponema pallidum particle agglutination (TPPA) leukocyte count,...
In total, 49 clinical samples were analyzed using two typing schemes, Enhanced Centers for Disease Control and Prevention (ECDC) multilocus sequence (MLST), to describe the molecular characteristics of circulating Treponema pallidum isolates in Xiamen between 2016 2017. addition, genetic mutations potentially related antibiotic resistance T. also analyzed. Forty five fully typed by ECDC, 14 different subtypes detected. The most common subtype was 16d/f (24.4%), followed 14d/f (20.0%). All...
The involvement of inflammasome activation and macrophage polarization during the process syphilis infection remains unknown. In this study, A series experiments were performed using human macrophages to research role NLRP3 regulation in interleukin (IL)-1β production its influence on triggered by T. pallidum.The results showed that M0 treated with pallidum, M1-associated markers inducible nitric oxide synthase (iNOS), IL-1β TNF-α upregulated, M2-associated CD206 IL-10 downregulated....
Abstract Background Elucidating the mechanism of macrophage phagocytic response will improve our knowledge host defence against Treponema pallidum . Objective To explore whether autophagy promotes T. phagocytosis and clearance via NLRP3 inflammasome in macrophages. Methods The interactions between role these processes ‐treated macrophages were investigated through experiments using human monocytic cell line (THP‐1)‐derived after was evaluated by inoculating rabbits with macrophage–treponeme...