A5042102713- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Carcinogens and Genotoxicity Assessment
- Bat Biology and Ecology Studies
- Genetically Modified Organisms Research
- DNA Repair Mechanisms
- Effects and risks of endocrine disrupting chemicals
- Plant Genetic and Mutation Studies
- Fish Ecology and Management Studies
- Skin and Cellular Biology Research
- Cellular Mechanics and Interactions
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Parasite Biology and Host Interactions
- Wildlife Ecology and Conservation
- RNA and protein synthesis mechanisms
- Hemoglobinopathies and Related Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Family Support in Illness
- Aquatic Invertebrate Ecology and Behavior
- Childhood Cancer Survivors' Quality of Life
- Cytokine Signaling Pathways and Interactions
- Biochemical and Molecular Research
Vanderbilt University
1978-2017
Carroll University
1995-2017
Vanderbilt University Medical Center
1986-2017
University of Kansas Medical Center
2017
University of Florida
2016
Reckitt Benckiser (United States)
2016
Vanderbilt-Ingram Cancer Center
2016
Columbus Oncology and Hematology Associates
2016
University of Louisville
2016
Sanford Burnham Prebys Medical Discovery Institute
2015
The 200-kD subunit of neurofilaments (NF-H) functions as a cross-bridge between and the neuronal cytoskeleton. In this study, four phosphorylated NF-H variants were identified major constituents axons from single neuron type, retinal ganglion cell, shown to have characteristics with different functional implications. We resolved Coomassie Blue-stained proteins apparent molecular masses 197, 200, 205, 210 kD on high resolution one-dimensional SDS-polyacrylamide gels mouse optic (optic nerve...
Significance Sex-determining region Y-related HMG box 2 (SOX2) is a well-established marker of neural stem and progenitor cells, its function was shown to be required for the self-renewal these cells. However, SOX2 in neuronal differentiation poorly understood. Here we described novel role which binds bivalently marked promoters poised proneural genes cells limits activity polycomb repressive complex excessive levels histone H3 Lys 27 trimethylation. We propose maintaining permissive...
Electrophoretic screening of (C57BL/6J x DBA/2J)F1 progeny male mice treated with N-ethyl-N-nitrosourea revealed a mouse that lacked the paternal carbonic anhydrase II (CA II). Breeding tests showed this trait was heritable and due to null mutation at Car-2 locus on chromosome 3. Like humans same inherited enzyme defect, animals homozygous for new allele are runted have renal tubular acidosis. However, prominent osteopetrosis found in CA deficiency could not be detected even very old mice. A...
The heat shock protein (HSP) 27 is constitutively expressed at low levels in medium-sized lumbar dorsal root ganglion (DRG) cells adult rats. Transection of the sciatic nerve results a ninefold upregulation HSP27 mRNA and axotomized neurons ipsilateral DRG 48 hr, without equivalent changes mRNAs encoding HSP56, HSP60, HSP70, HSP90. Dorsal rhizotomy, injuring central axon neuron, does not upregulate levels. After peripheral axotomy, are present small, medium, large neurons, transported...
Abstract Previous community psychology research has suggested that sense of can be a powerful explanatory tool for understanding development and individual well‐being, but not focused on the relationship environmental variables to construct. In other disciplines—especially urban planning—this construct received more attention it been empirically systematically studied. The three‐phase qualitative study reported here sampled people representative those who live work in Seaside, FL, town...
Peripheral nerve injury in neonatal rats results the death of majority axotomized sensory neurons by 7 d after injury. In adult animals, however, all survive for at least 4 months axotomy. How acquire capacity to axonal is not known. Here we describe how expression small heat shock protein 27 (HSP27) correlated with neuronal survival axotomy vivo and NGF withdrawal vitro. The number HSP27-immunoreactive L4 DRG low birth does change significantly 21 postnatal day 0 (P0) sciatic contrast,...
Male DBA/2J and C57BL/6J mice were treated with ethylnitrosourea (EtNU) at a dose of 250 mg/kg body weight. After sterile period 11 weeks, the animals mated untreated females; DBA C57 DBA. A small control group males was established, larger from earlier experiments also used for comparison. Tissue samples removed surgically parents F1 offspring examined by electrophoresis. Nine newly arisen mutants attributable to induction EtNU found in 22,512 loci tested mutation. No mutations encountered...
The phosphorylation and dephosphorylation of specific proteins was demonstrated directly in the intact vertebrate nervous system vivo. By exploiting neurons' ability to segregate a select group cytoskeletal from most other phosphorylated constituents cell by axoplasmic transport, we were able examine dynamics phosphate turnover on neurofilament mouse retinal ganglion neurons simultaneously labeled with [32P]orthophosphate [3H]proline Three [3H]proline-labeled protein (NFP) subunits,...
ADHD/ADD, once thought to occur only in children, is now recognized as continuing into adulthood many people. In order be labeled such, signs and symptoms must start before age 7 are primarily characterized by inattention, distractibility, impulsiveness. Although the exact mechanism unknown, a number of associated neurochemical structural abnormalities have been observed. This disorder can negatively affect educational, social, occupational lives those who suffer from its symptoms. It...
ABSTRACT A DBA/2 mouse treated with ethylnitrosourea sired an offspring whose hemoglobin showed extra band following starch gel electrophoresis. The variant migrated to a more cathodal position in gel. Isoelectric focusing indicated that chain 5 of the mutant than normal from mice and other α-globin, 1, was not affected. On gels phenotype allele, Hbay 9, expressed without dominance 5, 9 /Hbay homozygotes were fully viable laboratory. molecular basis for germinal mutation investigated by...
Ciliary neurotrophic factor (CNTF) and leukemia inhibitory (LIF) are members of a family neuropoietic cytokines that have broad range actions on many different neuronal populations. In cultured sympathetic neurons, CNTF LIF induce transcription the VIP other neuropeptide genes as part program differentiation. To gain insight into nuclear events involved in cytokine-mediated activation differentiation, we investigated mechanisms transcriptional vasoactive intestinal peptide (VIP) gene by...
Bats are unusual among mammals in having the ability to respond fluctuating climatic conditions by employing either a homeothermic or heterothermic strategy. Low temperatures affect insectivorous bats increasing energy required for homeothermy while roosting and decreasing available due lower activity of insect prey. may be particularly costly pregnant lactating because these experience greater energetic demands gestation milk production. The use heterothermy increase length decrease In...
Abstract: The neurotransmitter phenotype switch that occurs in cultures of rat superior cervical ganglion neurons after treatment with leukemia inhibitory factor or ciliary neurotrophic is a useful model permitting investigation the mechanisms cytokine‐mediated differentiation. Recently actions and have been linked through their interactions related receptor complexes. Here we compare effects these two cytokines on gene expression sympathetic neuronal begin to investigate mechanisms. We...