A5056871325- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Microbial Metabolic Engineering and Bioproduction
- Insect Pest Control Strategies
- Plant nutrient uptake and metabolism
- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Monoclonal and Polyclonal Antibodies Research
- Nitrogen and Sulfur Effects on Brassica
- Plant Molecular Biology Research
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- Lipid Membrane Structure and Behavior
- Algal biology and biofuel production
- Food Chemistry and Fat Analysis
- Entomopathogenic Microorganisms in Pest Control
- Coffee research and impacts
- Advanced Biosensing Techniques and Applications
- Insect Pheromone Research and Control
- Research in Cotton Cultivation
- Phytase and its Applications
- Insect symbiosis and bacterial influences
- Cell Image Analysis Techniques
Bio Base Europe
2020
Joint Research Center
2020
Bayer (Belgium)
2004-2017
Bayer (India)
2015
Bayer (United States)
2012
Leibniz Institute of Plant Genetics and Crop Plant Research
2011
Ghent University
1997
VIB-UGent Center for Plant Systems Biology
1993-1997
Abstract Constrained to develop within the seed, plant embryo must adapt its shape and size fit space available. Here, we demonstrate how this adjustment shapes metabolism of photosynthetic embryo. Noninvasive NMR-based imaging developing oilseed rape (Brassica napus) seed illustrates that, following bending, gradients in lipid concentration became established. These were correlated with local electron transport rate accumulation storage products. Experimentally induced changes morphology...
With growing populations and pressing environmental problems, future economies will be increasingly plant-based. Now is the time to reimagine plant science as a critical component of fundamental science, agriculture, stewardship, energy, technology healthcare. This effort requires conceptual technological framework identify map all cell types, comprehensively annotate localization organization molecules at cellular tissue levels. framework, called Plant Cell Atlas (PCA), for understanding...
Seeds provide the basis for many food, feed, and fuel products. Continued increases in seed yield, composition, quality require an improved understanding of how developing converts carbon nitrogen supplies into storage. Current knowledge this process is often based on premise that transcriptional regulation directly translates via enzyme concentration flux. In attempt to highlight metabolic control, we explore genotypic differences partitioning vitro cultured embryos oilseed rape (Brassica...
We report the purification, cloning and characterization of an aminopeptidase N from midgut epithelium Manduca sexta that binds Cry1Ab5, insecticidal crystal protein [ICP] Bacillus thuringiensis. Sequence information derived this M. was used for brush‐border membrane Plutella xylostella , insect species which some populations acquired resistance against Cry1Ab5. Affinity chromatography on a Cry1Ab5 matrix to isolate 120‐kDa glycoprotein larval lepidopteran sexta. On ligand blots purified...
An attempt has been made to define the extent which metabolic flux in central plant metabolism is reflected by changes transcriptome and metabolome, based on an analysis of vitro cultured immature embryos two oilseed rape (Brassica napus) accessions contrast for seed lipid accumulation. Metabolic (MFA) was used constrain a balance model included 671 biochemical transport reactions within metabolism. This highly confident information eventually comparative vs. transcript (metabolite)....
Binding of three Bacillus thuringiensis insecticidal crystal proteins (ICPs) to the midgut epithelium Ostrinia nubilalis larvae was characterized by performing binding experiments with both isolated brush border membrane vesicles and gut tissue sections. Our results demonstrate that two independent ICP receptors are present in O. epithelium. From competition performed 125 I-labeled native ICPs it concluded CryIA(b) CryIA(c) recognized same receptor. An 11-fold-higher affinity for this...
SUMMARY Many pathways of primary metabolism are substantially conserved within and across plant families. However, significant differences in organization fluxes through a reaction network may occur, even between plants closely related genera. Assessing understanding these is key to appreciating metabolic diversity, attempts engineer for higher crop yields desired product profiles. To better understand lipid seed oil synthesis canola ( Brassica napus ), we have characterized four homologues...
Producing healthy, high-oleic oils and eliminating trans-fatty acids from foods are two goals that can be addressed by reducing activity of the oleate desaturase, FAD2, in oilseeds. However, it is essential to understand consequences FAD2 on metabolism, cell biology physiology oilseed crop plants. Here, we translate knowledge studies fad2 mutants Arabidopsis (Arabidopsis thaliana) investigate limits non-GMO approaches maximize oleic acid seed oil canola (Brassica napus), a species expresses...
Biotinylation of Bacillus thuringiensis insecticidal crystal proteins (ICPs) was evaluated for its potential use in an alternative ICP screening method and the characterization receptors. In vivo biological activity CryIA(b), as inferred from bioassays with Manduca sexta Ostrinia nubilalis histopathological effects on O. midgut cells induced by force feeding, not affected biotinylation at moderate ratios. A competitive radioreceptor assay showed that there only a minor reduction binding...
DGAT1 enzymes (acyl-CoA:diacylglycerol acyltransferase 1, EC 2.3.1.20) catalyse the formation of triacylglycerols (TAGs), most abundant lipids in vegetable oils. Thorough understanding enzymology oil accumulation is critical to goal modifying oilseeds for improved production. Four isoforms BnDGAT1, final and rate-limiting step triacylglycerol synthesis, were characterized from Brassica napus, one world's important oilseed crops. Transcriptional profiling developing B. napus seeds indicated...
The tradeoff between protein and oil storage in oilseed crops has been tested here rape (Brassica napus) by analyzing the effect of suppressing key genes encoding products (napin cruciferin). phenotypic outcomes were assessed using NMR mass spectrometry imaging, microscopy, transcriptomics, proteomics, metabolomics, lipidomics, immunological assays, flux balance analysis. Surprisingly, profile was only moderately changed RNA interference transgenics. However, embryonic cells had undergone...
ABSTRACT Directed evolution of a cyanobacterial Δ9 fatty acid desaturase (DSG) from Synechococcus elongatus , PCC6301 created new, more productive desaturases and revealed the importance certain amino residues to increased desaturation. A codon‐optimized DSG open reading frame with an endoplasmic‐reticulum retention/retrieval signal appended was used as template for random mutagenesis. Increased desaturation detected using novel screen based on complementation unsaturated auxotrophy...
β-Glucuronidase (GUS) is a useful reporter for the evaluation of promoter characteristics in transgenic plants. Here, we introduce an original technique to quantify strength promoters at subtissue resolution cell clusters. The method combines cryotomy, laser microdissection, and improved fluorometric analysis GUS activity using 6-chloro-4-methylumbelliferyl-β-d-glucuronide as efficient fluorogenic substrate kinetic studies microdissection/6-chloro-4-methylumbelliferyl-β-d-glucuronide robust...
Bayer CropScience is a leader in the oilseed rape seeds business with 2013 market share of 50% Canada, based on creation and use unique hybridization system enabling development high-yielding canola (B.napus) InVigor® hybrids. For European markets, developing non-transgenic hybrids that will be complemented differentiating traits. To this end, highly effective mutagenesis-based omics-directed reverse genetics platform was established which enables novel productivity traits canola.The process...
Phage display has proven to be an invaluable instrument in the search for proteins and peptides with optimized or novel functions. The amplification selection of phage libraries typically involve several operations handling large bacterial cultures during each round. Purification assembled particles after rescue adds labor time demand. authors therefore devised a method, termed situ evaluation (RISE), which combines all steps from binding single microwell. To test this concept, wells were...