A5102778051- Zebrafish Biomedical Research Applications
- Neural dynamics and brain function
- Photosynthetic Processes and Mechanisms
- Retinal Development and Disorders
- Cellular transport and secretion
- Visual perception and processing mechanisms
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Neuroscience and Neuropharmacology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Protist diversity and phylogeny
- Electronic Packaging and Soldering Technologies
- Plant Gene Expression Analysis
- Protein Degradation and Inhibitors
- Advanced Fluorescence Microscopy Techniques
- Nanofabrication and Lithography Techniques
- Advanced ceramic materials synthesis
- Aluminum Alloys Composites Properties
- Neonatal and fetal brain pathology
- 3D IC and TSV technologies
- Neurobiology and Insect Physiology Research
- Erythrocyte Function and Pathophysiology
- Graphite, nuclear technology, radiation studies
- Postharvest Quality and Shelf Life Management
King's College London
2011-2018
University of Warwick
2007-2017
Guy's Hospital
2011-2014
Zero to Three
2011
Shipley Hospital
1995
Coventry (United Kingdom)
1995
Abstract We generated fusions between three Arabidopsis (Arabidopsis thaliana) tonoplast intrinsic proteins (TIPs; α-, γ-, and δ-TIP) yellow fluorescent protein (YFP). also produced soluble reporters consisting of the monomeric red (RFP) either C-terminal vacuolar sorting signal phaseolin or sequence-specific proricin. In transgenic leaves, mature roots, root tips, all TIP localized to central vacuole both lumenal RFP were found within TIP-delimited vacuoles. embryos from developing, mature,...
We have cloned a member of the reticulon (RTN) family Arabidopsis thaliana (RTNLB13). When fused to yellow fluorescent protein (YFP) and expressed in tobacco leaf epidermal cells, RTNLB13 is localized endoplasmic reticulum (ER). Coexpression soluble ER luminal marker reveals that YFP‐tagged, myc‐tagged or untagged induces severe morphological changes lumen ER. show, using fluorescence recovery after photobleaching (FRAP) analysis, overexpression greatly reduces diffusion proteins within...
Pathophysiological explanations of epilepsy typically focus on either the micro/mesoscale (e.g. excitation-inhibition imbalance), or macroscale network architecture). Linking abnormalities across spatial scales remains difficult, partly because technical limitations in measuring neuronal signatures concurrently at involved. Here we use light sheet imaging larval zebrafish brain during acute epileptic seizure induced with pentylenetetrazole. Spectral changes spontaneous activity are then...
Abstract Background Tonoplast intrinsic proteins (TIPs) are widely used as markers for vacuolar compartments in higher plants. Ten TIP isoforms encoded by the Arabidopsis genome. For several isoforms, tissue and cell specific pattern of expression not known. Results We generated fluorescent protein fusions to genomic sequences all members family whose is predicted occur root tissues (TIP1;1 1;2; TIP2;1, 2;2 2;3; TIP4;1) expressed these fusions, both individually selected pairwise...
How local circuits within the brain process visual information has classically been addressed at single neuron level. Such reductionist approaches, however, struggle to capture full scope of functional properties associated with even "simple" nuclei. Using population calcium imaging, we aim describe how zebrafish optic tectum information. Specifically, are previously identified direction-selective (DS) and orientation-selective (OS) retinal ganglion cell (RGC) inputs (Nikolaou et al., 2012)...
We have examined the form, diversity, and organization of three functional classes retinal inputs to zebrafish optic tectum during development. Our systems-based approach was analyze data from populations ganglion cells labeled with a presynaptic targeted calcium indicator, synaptophysin GCaMP3 (SyGCaMP3). Collectively, our findings provide an insight as degree visual encoding retino-tectal development how it dynamically evolves nascent noisy neural-scape increasingly complex refined...
The plant vacuolar sorting receptor (VSR) binds proteins carrying signals (VSS) of the ‘sequence‐specific’ type (ssVSS) but not C‐terminal, hydrophobic (ctVSS). Seeds Arabidopsis mutants lacking major VSR isoform, AtVSR1, secrete a proportion destined to storage vacuoles. for these are well defined, they do seem be ssVSS type. Here, we tested whether absence VSR1 in seeds leads secretion reporter ctVSS. Our results show that reporters either or ctVSS equally secreted VSR1. We discuss our...
Members of the Cadm/SynCAM/Necl/IGSF/TSLC family cell adhesion molecules are known to have diverse functions during development nervous system, but information regarding their role central system (CNS) in vivo is scarce. The rapid a relatively simple larval zebrafish makes them highly tractable model organism for studying gene function development. An essential prerequisite functional studies description protein localization. To address this we generated subtype-specific antibodies two...
Targeting protein stability with small molecules has emerged as an effective tool to control abundance in a fast, scalable and reversible manner. The technique involves tagging of interest (POI) destabilizing domain (DD) specifically controlled by molecule. successful construction such fusion proteins may, however, be limited functional interference the DD epitope electrostatic interactions required for full biological function proteins. Another drawback this approach is remaining endogenous...
Summary Pathophysiological explanations of epilepsy typically focus on either the micro/mesoscale (e.g. excitation-inhibition imbalance), or macroscale network architecture). Linking abnormalities across spatial scales remains difficult, partly because technical limitations in measuring neuronal signatures concurrently at involved. Here we use light sheet imaging larval zebrafish brain during acute epileptic seizure induced with pentylenetetrazole. Empirically measured spectral changes...
Like the conventional PCB, Moulded Interconnect Device (MID), be it 2‐ or 3‐dimensional, will have, as its interconnection medium, conductive patterning structuring upon surface of device. This pattern will, in majority MID products, take form a metal (usually copper) deposited plastic. How this is and remains attached to critical step production MID. That bond, adhesion, between plastic applied circuitry. Without strong reliable bond circuitry not possible manufacture with characteristics...