Cyclophilin B is targeted to the secretory pathway via an endoplasmic reticulum signal sequence. We analyzed localization and trafficking of endogenous transfected cyclophilin in mammalian cells. accumulates both complexes on plasma membrane. The immunosuppressant cyclosporin A specifically mobilizes from reticulum, promotes secretion into medium. suggest that competes with membrane proteins for association pathway. These findings argue favor a role as chaperone destined membrane, rather...

Secretory leukocyte protease inhibitor (SLPI) protects tissue from proteases, and promotes cell proliferation healing during inflammatory response. SLPI is also overexpressed in gastric, lung ovarian cancers, which accelerates the metastasis of cancer cells. Matrix metalloproteinases-2, -9 (MMP-2 MMP-9) are high metastatic promote migration cells through collagen degradation. MMP-2, critical factors stimulating processes but there no reports a direct correlation between these molecules....

Of 360° Virtual Reality (VR) is possibly produced and sufficiently effective as a consumer-friendly VR learning medium. Therefore, it also expected to be useful in the dental practice field, self-learning medium for non-face-to-face skill training during ongoing pandemic (COVID-19). Accordingly, this study was conducted assess media periodontal instrument operation.We recruited 30 participants who had never experienced training. We offered basic education initial assessment (IA), then...

We have previously shown that parasite eggs been identified in the coprolites of Korean mummies. These shed light on parasitic infection patterns populations living several hundred years ago. conducted a scanning electron microscopy (SEM) study ancient Trichuris trichiura, Ascaris lumbricoides, Metagonimus yokogawai, Paragonimus westermani, and Gymnophalloides seoi recovered from mummies Joseon Dynasty. anticipated taphonomic conditions mummification would alter certain species but not...

Sulfasalazine (SSZ) is an anti-inflammatory drug that has been used to treat inflammatory bowel disease and rheumatoid arthritis for decades. Recently, some reports have suggested SSZ also anti-cancer properties against human tumors. However, little known about the effects of on oral cancer. The aim this study was investigate in squamous cell carcinoma (OSCC) cells elucidate mechanisms involved. authors investigated anti-proliferative effect using MTT method HSC-4 (an OSCC line). Cell cycle...

Abstract We have developed a novel culture technique for human embryonic stem cells (hESCs) using porous membrane with feeder cells. The were seeded and attached to the bottom of and, subsequently, hESCs cultured on top membrane. This (PMT) allowed be successfully effectively efficiently separated from cell layer without enzyme treatment. being by PMT observed interact through pores membrane, where interaction was dependent pore size used. It also revealed that number hESC colonies depended...

Abstract The socio‐cultural antipathies of some descendants with regard to invasive examinations age‐old human remains make permission for dissection Korean mummies the Joseon Dynasty (1392–1910) difficult obtain. Overcoming this obstacle necessitated use non‐invasive techniques, such as multi‐detector computerized tomography (MDCT) and endoscopic examination, enabling determination preservation status internal organs without significantly damaging themselves. However, MDCT alone cannot...

Mineralized bone matrix constituted with collagenous and non-collagenous proteins was synthesized by osteoblasts differentiated from mesenchymal stem cells. Secretory leukocyte protease inhibitor (SLPI), a serine inhibitor, promotes cell migration proliferation, suppresses the inflammatory response. Recent studies reported that SLPI regulates formation of dentin mineralization odontoblasts increases adhesion viability preosteoblasts on titanium (Ti) surface. Ti its alloys are widely used...

Titanium (Ti) is a widely used biomaterial for dental implants because of its outstanding biocompatibility hard tissues. Osseointegration, the interaction between implanted biomaterials and living cells in bone, essential successful implantation. Rosmarinic acid (RA) plant-derived phytochemical with low toxicity side effects has various that can be applied as therapeutic substance. The MC3T3-E1 osteoblastic on Ti surface medium or without 14 μg/ml RA were to test osteoblast differentiation,...

본 연구는 시판중인 에너지음료에 의한 치아 법랑질 침식효과를 알아보기 위하여 선호도가 높은 4종의 에너지음료를 선정하여 산도, 완충능 및 Ca, P 함량을 분석하였다. 또한 에너지음료 침지 전 후의 법랑질의 침식 정도를 표면미세경도와 SEM으로 관찰하여 다음과 같은 결론을 얻었다. 1. 에너지음료의 산성도는 번인텐스에서 <TEX>$2.78{\pm}0.01$</TEX>로 가장 낮았고 대조군인 증류수에서 <TEX>$6.475{\pm}0.01$</TEX>로 높았다. 완충능은 번인텐스가 pH 5.5에서 <TEX>$3.48{\pm}0.155$</TEX>이고, 7.0에서는 <TEX>$1.88{\pm}0.15$</TEX>로 높게 나타났고, 핫식스에서 각각 <TEX>$1.71{\pm}0.37$</TEX>, <TEX>$1.23{\pm}0.35$</TEX>로 낮게 나타났다. 2. 칼슘 함유량은 레드불(<TEX>$77.21{\pm}2.70mg/kg$</TEX>)에서 나타났고...

To assess the association between chewing difficulty and symptoms of depression in a representative sample Korean population.Cross-sectional.Korea National Health Nutrition Examination Survey (KNHANES).KNHANES participants (N = 5,158).Chewing was assessed according to self-reported presence problems using structured questionnaire. Symptoms were defined as having feelings sadness or consecutively over 2 weeks during last 12 months. Multivariable logistic regression analysis used determine...

As a significant association has been established between residual ancient DNA (aDNA) and histological preservation, the morphological identification or confirmation of preserved cell residue in tissues would greatly facilitate aDNA studies enhance definitiveness their conclusions. However, differentiation from other tissue structures always difficult, even for experienced histologists, due to severe degradation cells over long burial durations. In present study, using fluorescence...

In periodontitis, alveolar bone resorption is induced by excessive host immune and inflammatory response against bacterial infection. Secretory leukocyte protease inhibitor (SLPI) has anti-bacterial anti-inflammatory activity in responses. SLPI inhibits joint inflammation destruction, but the function of periodontitis unclear. Therefore, this study investigated whether LPS-induced rats. Micro-computed tomography histological analysis showed that inhibited periodontitis. Immunohistochemistry...

Abstract The two SH3 domains and one SH2 domain containing adaptor protein Grb2 is an essential element of the Ras signaling pathway in multiple systems. recognizes interacts with phosphotyrosine residues on activated tyrosine kinases, whereas bind to several proline‐rich domain‐containing proteins such as Sos1. To define difference Grb2‐associated hepatocarcinoma cells, we performed coprecipitation analysis using recombinant GST‐Grb2 fusion found that components (p170, p125, p100, p80)...

Secretory leukocyte protease inhibitor (SLPI) plays an important role in promoting the invasion and metastasis of a range cancer cells. However, there are no reports expression function SLPI oral carcinoma In this study, cell line KB was used to determine whether affects proliferation, migration RT-PCR Western blotting revealed high levels endogenous cells as well strong increase secretion after wounding compared immortalized normal keratinocytes (INOK). The wound healing assay more than...

본 연구는 천연한방 식물 추출물의 치아우식 예방제제로서 가능성을 평가하고자 구강 양치용액과 함께 S. mutans에 대한 항균활성을 측정하고, 정상인 치은섬유모세포에 독성 유무를 평가하여 다음과 같은 결과를 얻었다. 용매별 최소성장억제농도는 증류수보다 에탄올 추출물에서 우수한 성장억제 효과를 보였다. 최소성장억제농도를 비교한 결과, 추출물은 보골지가, 구강양치액은 헥사메딘이 가장 낮은 농도에서 최소살균농도를 헥사메딘과 가그린이 사멸효과를 나타냈다. 정상인의 추출물을 최소성장억제농인 10, 39, $78{\mu}g/ml$ 로 처리하여 측정한 결과 세포독성이 없었다. 그러나 최소살균농도 값을 토대로 각 추출물별 농도를 달리하여 처리한 보골지를 제외하고는 다른 농도가 높아짐에 따라 있는 것으로 나타났다. 이상의 종합하면, 4종의 합성 화학물질인 구강양치액과 비교 시 유사한 항균효과가 있었다. 따라서 적정 첨가함으로써 대체 예방제제로 실용화할 수 있을 생각된다. 【This study...

본 연구는 개인의 행동 변화를 설명하는 이론적 모형인 건강신념모형(health belief model)을 적용해 임신부의 구강건강관련행위에 영향을 미치는 요인을 분석하여 구강건강증진과 구강보건교육프로그램 개발의 기초자료를 제공할 목적으로 시행하였다. 2014년 8월 1일부터 31일까지 서울특별시, 경기도, 인천광역시에 소재한 8개의 산부인과의원과 1개의 산모교실을 방문한 임신부 총 217명을 대상으로 자기기입식 설문지를 배부하였다. 수집된 자료는 PASW Statistics ver. 18.0 프로그램을 이용하여 다음과 같은 결론을 얻었다. 건강신념 수준은 3.61점이었으며 인지된 유익성이 4.37점으로 가장 높았고 자기 효능감 4.04점, 민감성 3.98점, 장애성 3.15점 순이었으며 심각성이 2.91점으로 낮았다. 건강신념요인과 구강건강관련행위의 상관관계로 심각성은 치아에 좋은 식품섭취 노력, 임신 중 구강검진과 양의 상관관계를 보였으며 유익성은 보였다. 장애성은 매 간식...

This study aimed to compare the effectiveness of chewable toothbrush and manual provide basic data for recommendation in specific groups situations. A total 20 subjects participated this (rolling method, 10; non-rolling 10). After professional prophylaxis, participants used brush their teeth 3 minutes. a 7-day wash-out period, according manufacturer's instructions. Pre- post-plaque indexing was performed. The dental plaque index assessed using Turesky Modification Quigley-Hein Plaque Index...

Thymosin β4 (Tβ4) is known to inhibit an inflammatory response and increase the survival of osteoblasts on titanium (Ti) surfaces. Ti most widely used graft material in dentistry; however, induced following implant placement results generation reactive oxygen species (ROS). The oxidative stress from production ROS such as nitric oxide (NO) hydrogen peroxide (H2O2) can damage surrounding cells, resulting failure by decreasing cell viability. Thus, aim this study was determine biological...

Moon-Jin Jeong, Myoung-Hwa Lee, Soon-Jeong So-Jeong Kim, Myeong-Ji Ko, Hye-Won Sim, Ju-Young Ae-Jung Im, and Do-Seon Lim. J Dent Hyg Sci 2019;19:261-70. https://doi.org/10.17135/jdhs.2019.19.4.261

Moon-Jin Jeong, Han-A Cho, Su-Yeon Kim, Ka-Rim Kang, Eun-Bin Lee, Ye-Ji Jung-Hyeon Choi, Ki-Sung Kil, Myoung-Hwa Soon-Jeong and Do-Seon Lim. J Dent Hyg Sci 2018;18:164-71. https://doi.org/10.17135/jdhs.2018.18.3.164

본 연구는 시판중인 국내외 제조 플레인 탄산수 5종을 선정하여 우치 법랑질 침식 및 그에 따른 세균 침착 정도를 확인하였다. 탄산수에 시편을 침지 후 용출된 칼슘 농도를 측정하고, SEM을 이용하여 표면의 침식을 관찰하였다. 이 S. mutans 균과 sorbinus 균이 접종된 배지에 함께 배양하여 침식된 시편의 확인하여 다음과 같은 결론을 얻었다. 실험에 사용된 5종은 수소이온농도가 pH 5.5보다 낮았고, 치아의 칼슘의 용출이 확인되었다. 이용한 치면 분석 결과, 용출은 의한 침식이 원인임을 확인하였고, 두 방법을 통해 인공 탄산수와 광천수간의 뚜렷한 활성 차이는 관찰되지 않았다. 또한 의해 유발된 치아 침식으로 침착이 용이해지는 것으로 이런 결과들로부터 음용 시 유발될 수 있으며 구강 내 가중될 있으므로, 가능성에 대한 소비자의 인식이 필요하며, 탄산수를 물 대체음료 정도로 생각하기보다는 무가당 콜라와 탄산음료에 준하는 범주로 포괄하여 음용하는 것이 바람직하다고...