A5085912890- Sarcoma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Protein Degradation and Inhibitors
- RNA modifications and cancer
- Macrophage Migration Inhibitory Factor
- Cancer-related gene regulation
- Cell death mechanisms and regulation
- Neuroblastoma Research and Treatments
- Apelin-related biomedical research
- Virus-based gene therapy research
- Signaling Pathways in Disease
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
Red de Investigación en Actividades Preventivas y Promoción de la Salud
2023-2025
Sant Joan de Déu Research Foundation
2020-2022
Institut de Recerca Sant Joan de Déu
2020-2022
Hospital Sant Joan de Déu Barcelona
2020-2022
ABSTRACT Ewing sarcoma (ES) is an aggressive bone and soft tissue neoplasm characterized by EWSR1/ETS rearrangements whose cellular origin remains unclear. EWS-FLI1 expression in human pediatric mesenchymal stem cells (hpMSCs) induces a quantitatively qualitatively different transcriptional response than its adult MSCs (haMSCs), but fails to form tumors vivo . ES have early developmental lineage signatures distinct from postnatal MSCs. Here, we generated experimental teratomas out of...
RING1B is necessary for the expression of key EWSR1-FLI1 targets by promoting oncogene recruitment to enhancers.
The bone marrow (BM) is the most common site of dissemination in patients with aggressive, metastatic neuroblastoma (NB). However, molecular mechanisms underlying aggressive behavior NB cells BM niche are still greatly unknown. In present study, we explored biological that play a critical role cell survival and progression investigated potential therapeutic targets.Patient-derived primary cultures were generated using fresh aspirates obtained from patients. lines cultured presence...
Abstract Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in childhood and adolescence. Refractory/relapsed RMS patients present a bad prognosis that combined with lack of specific biomarkers impairs development new therapies. Here, we utilize dynamic BH3 profiling (DBP), functional predictive biomarker measures net changes mitochondrial apoptotic signaling, to identify anti-apoptotic adaptations upon treatment. We employ this information guide use mimetics specifically inhibit...
Targeted agents have emerged as promising molecules for cancer treatment, but most of them fail to achieve complete tumor regression or attain durable remissions due adaptations. We used dynamic BH3 profiling identify targeted effectiveness and anti-apoptotic adaptations upon treatment in rhabdomyosarcoma. focused on studying the use mimetics specifically inhibit pro-survival BCL-2 family proteins, overwhelm resistance therapy prevent relapse. observed that MEK1/2 inhibitor trametinib...
Abstract Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in childhood and adolescence. Refractory/relapsed RMS patients present a bad prognosis that combined with lack of specific biomarkers difficult development new therapies. We here utilize dynamic BH3 Profiling (DBP), functional predictive biomarker measures net changes mitochondrial apoptotic signaling, to identify anti-apoptotic adaptations upon treatment. use this information guide mimetics specifically inhibit BCL-2...
SUMMARY Ewing Sarcoma (EwS) is an aggressive bone and soft tissue tumor driven by the fusion oncoprotein EWSR1-FLI1. This aberrant transcription factor binds to GGAA microsatellites, causing epigenetic reprogramming through formation of active neo-enhancers in a permissive cellular context. Inhibition oncogene remains challenging current efforts instead seek exploit emergent treatments targeting EWSR1-FLI1 cofactors. Here, stemming from genome-wide redistribution H3K27me3 upon expression...