A5061265109- Neuroblastoma Research and Treatments
- Sarcoma Diagnosis and Treatment
- Protein Degradation and Inhibitors
- Muscle Physiology and Disorders
- Hedgehog Signaling Pathway Studies
- RNA Interference and Gene Delivery
- Tumors and Oncological Cases
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Chromatin Remodeling and Cancer
- Ubiquitin and proteasome pathways
- Caveolin-1 and cellular processes
- Cancer, Hypoxia, and Metabolism
- Tissue Engineering and Regenerative Medicine
- Signaling Pathways in Disease
- Cancer Genomics and Diagnostics
- Cancer-related Molecular Pathways
- Circular RNAs in diseases
- Lung Cancer Diagnosis and Treatment
- Renal and related cancers
- Metabolism and Genetic Disorders
- Single-cell and spatial transcriptomics
- Glioma Diagnosis and Treatment
Vall d'Hebron Institut de Recerca
2015-2024
Universitat Autònoma de Barcelona
2014-2024
Baptist Cancer Center
2023
Vall d'Hebron Hospital Universitari
2003-2018
Hospital Materno-Infantil
2002-2009
Vlaams Instituut voor Biotechnologie
2007
Transgene (France)
2007
Children's Hospital of Philadelphia
2006
King's College London
2001
Hospital de Sant Pau
2001
Rhabdomyosarcoma (RMS) is a group of pediatric cancers with features developing skeletal muscle. The cellular hierarchy and mechanisms leading to developmental arrest remain elusive. Here, we combined single-cell RNA sequencing, mass cytometry, high-content imaging resolve intratumoral heterogeneity patient-derived primary RMS cultures. We show that the aggressive alveolar (aRMS) subtype contains plastic muscle stem-like cells cycling progenitors drive tumor growth, subpopulation...
Osteosarcoma is a type of bone tumour characterized by considerable levels phenotypic heterogeneity, aneuploidy, and high mutational rate. The life expectancy osteosarcoma patients has not changed during the last three decades thus much remains to be learned about disease biology. Here, we employ RGB-based single-cell tracking system study clonal dynamics occurring in de novo-induced murine model. We show that cells present initial polyclonal dynamics, followed dominance associated with...
Duchenne muscular dystrophy (DMD) is a fatal and incurable muscle degenerative disorder. We identify function of the protease urokinase plasminogen activator (uPA) in mdx mice, mouse model DMD. The expression uPA induced dystrophic muscle, genetic loss mice exacerbated reduced function. Bone marrow (BM) transplantation experiments revealed critical for BM-derived repair via three mechanisms: (1) by promoting infiltration inflammatory cells; (2) preventing excessive deposition fibrin; (3)...
Current therapies for most non-infectious diseases are directed at or affect functionality of the human translated genome, barely 2% all genetic information. By contrast, therapeutic potential targeting transcriptome, ~ 70% remains largely unexplored. RNA therapeutics is an emerging field that widens range druggable targets and includes elements such as microRNA. Here, we sought to screen microRNA with tumor-suppressive functions in neuroblastoma, aggressive pediatric tumor sympathetic...
Abstract Purpose: Rhabdomyosarcoma (RMS) is the most common type of soft tissue sarcoma in children and can be divided into two main subtypes: embryonal alveolar RMS. Patients with metastatic disease continue to have very poor prognosis although aggressive therapies recurrences are advanced localized disease. The oncogenic potential Notch pathway has been established some cancers adult pediatric malignancies. Experimental Design: A real-time PCR assay was used ascertain expression several...
Rhabdomyosarcoma (RMS) is a pediatric soft tissue sarcoma arising from myogenic precursors that have lost their capability to differentiate into skeletal muscle. The polycomb-group protein EZH2 Lys27 histone H3 methyltransferase regulates the balance between cell proliferation and differentiation by epigenetically silencing muscle-specific genes. often over-expressed in several human cancers acting as an oncogene. We previously reported inhibition induces cycle arrest followed of RMS cells...
// Aroa Soriano 1 , Laia París-Coderch Luz Jubierre Alba Martínez 2 Xiangyu Zhou 3 Olga Piskareva 4 Isabella Bray Isaac Vidal Ana Almazán-Moga Carla Molist Josep Roma José R. Bayascas Oriol Casanovas Raymond L. Stallings Sánchez de Toledo Soledad Gallego Miguel F. Segura Laboratory of Translational Research in Child and Adolescent Cancer, Vall d'Hebron Institute (VHIR)-UAB, Barcelona, Spain Tumor Angiogenesis Group, Catalan Oncology-IDIBELL, L'Hospitalet Llobregat, Institut Neurociències...
Rhabdomyosarcoma (RMS) is the most common type of soft tissue sarcoma in children. Regarding histopathological criteria, RMS can be divided into 2 main subtypes: embryonal and alveolar. These subtypes differ considerably their clinical phenotype molecular features. Abnormal regulation or mutation signalling pathways that regulate normal embryonic development such as Notch, Hedgehog, Wnt a recurrent feature tumorigenesis. Herein, general features each three pathways, implication cancer...
Abstract MicroRNAs (miRNAs) are small non‐coding endogenous RNAs, which attracting a growing interest as therapeutic molecules due to their central role in major diseases. However, the transformation of these biomolecules into drugs is limited unstability bloodstream, caused by nucleases abundantly present blood, and poor capacity enter cells. The conjugation miRNAs nanoparticles (NPs) could be an effective strategy for clinical delivery. Herein, engineering non‐liposomal lipid nanovesicles,...
Rhabdomyosarcoma (RMS) is the most common type of soft tissue sarcoma in children. The Hedgehog (HH) pathway known to develop an oncogenic role RMS. However, molecular mechanism that drives activation RMS not well understood. expression HH ligands was studied by qPCR, western blot and immunohistochemistry. Functional animal model studies were carried out with cells transduced shRNAs against or treated HH-specific inhibitors (Vismodegib MEDI-5304). Finally, characterisation off-target effect...
Abstract Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in childhood and adolescence. Refractory/relapsed RMS patients present a bad prognosis that combined with lack of specific biomarkers impairs development new therapies. Here, we utilize dynamic BH3 profiling (DBP), functional predictive biomarker measures net changes mitochondrial apoptotic signaling, to identify anti-apoptotic adaptations upon treatment. We employ this information guide use mimetics specifically inhibit...
Rhabdomyosarcoma (RMS) is the commonest type of soft-tissue sarcoma in children. Patients with metastatic RMS continue to have very poor prognosis. Recently, several works demonstrated a connection between Notch pathway activation and regulation cell motility invasiveness. However, molecular mechanisms this possible relationship remain unclear.The was manipulated pharmacologically genetically. The mRNA changes were analysed by quantitative PCR protein variations western blot...
// Juan Huertas-Martínez 1 , Santiago Rello-Varona David Herrero-Martín Ignasi Barrau Silvia García-Monclús Miguel Sáinz-Jaspeado Laura Lagares-Tena Yaiza Núñez-Álvarez 5 Mateo-Lozano 2 Jaume Mora Josep Roma 3 Nuria Toran Sebastian Moran 4 Roser López-Alemany Soledad Gallego Manel Esteller A. Peinado Xavier García del Muro and Oscar M. Tirado Sarcoma research group, Molecular Oncology Lab, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain...