A5019378645- MicroRNA in disease regulation
- Neuroblastoma Research and Treatments
- Protein Degradation and Inhibitors
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Cell death mechanisms and regulation
- Circular RNAs in diseases
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Sarcoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Autophagy in Disease and Therapy
- Chromatin Remodeling and Cancer
- Signaling Pathways in Disease
- Cancer-related Molecular Pathways
- Breast Cancer Treatment Studies
- Tumors and Oncological Cases
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Endoplasmic Reticulum Stress and Disease
- Glioma Diagnosis and Treatment
- Genetics and Neurodevelopmental Disorders
- Immunotherapy and Immune Responses
Vall d'Hebron Institut de Recerca
2016-2025
Universitat Autònoma de Barcelona
2014-2025
Eastern Cooperative Oncology Group
2009-2023
Hospital Del Mar
2008-2021
Parc de Salut
2020
New York University
2009-2017
NYU Langone Health
2010-2015
Icahn School of Medicine at Mount Sinai
2013-2015
Instituto de Salud Carlos III
2013
Columbia University Irving Medical Center
2011
The highly aggressive character of melanoma makes it an excellent model for probing the mechanisms underlying metastasis, which remains one most difficult challenges in treating cancer. We find that miR-182, member a miRNA cluster chromosomal locus (7q31-34) frequently amplified melanoma, is commonly up-regulated human cell lines and tissue samples; this up-regulation correlates with gene copy number subset lines. Moreover, miR-182 ectopic expression stimulates migration cells vitro their...
Metastatic melanoma remains a mostly incurable disease. Although newly approved targeted therapies are efficacious in subset of patients, resistance and relapse rapidly ensue. Alternative therapeutic strategies to manipulate epigenetic regulators disrupt the transcriptional program that maintains tumor cell identity emerging. Bromodomain extraterminal domain (BET) proteins epigenome readers known exert key roles at interface between chromatin remodeling regulation. Here, we report BRD4, BET...
To identify a melanoma microRNA (miRNA) expression signature that is predictive of outcome and then evaluate its potential to improve risk stratification when added the standard-of-care staging criteria.Total RNA was extracted from 59 formalin-fixed paraffin-embedded metastases hybridized miRNA arrays containing 911 probes. We correlated with post-recurrence survival other clinicopathologic criteria.We identified 18 miRNAs whose overexpression significantly longer survival, defined as more...
Vitamin D deficiency is associated with the high risk of colon cancer and a variety other diseases. The active vitamin metabolite 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) regulates gene transcription via its nuclear receptor (VDR), posttranscriptional regulatory mechanisms expression have also been proposed. We identified microRNA-22 (miR-22) several miRNA species as 1,25(OH)2D3 targets in human cells. Remarkably, miR-22 induced by time-, dose- VDR-dependent manner. In SW480-ADH HCT116 cells,...
Molecular pathogenesis of high-grade serous ovarian carcinoma (HG-SOC) is poorly understood. Recent recognition HG-SOC precursor lesions, defined as tubal intraepithelial (STIC) in fimbria, provides a new venue for the study early genetic changes HG-SOC. Using microRNA profiling analysis, we found that miR-182 expression was significantly higher STIC than matched normal Fallopian tube. Further revealed overexpressed most cases. To test whether plays major role tumourigenesis HG-SOC,...
Abstract The transforming growth factor β (TGFβ) pathway plays critical roles during cancer cell epithelial-mesenchymal transition (EMT) and metastasis. SMAD7 is both a transcriptional target negative regulator of TGFβ signalling, thus mediating feedback loop that may potentially restrain responses cells. Here, however, we show treatment induces transcription but not its protein level in panel Mechanistic studies reveal activates the expression microRNA-182 (miR-182), which suppresses...
ABTL0812 is a first-in-class small molecule with anti-cancer activity, which currently in clinical evaluation phase 2 trial patients advanced endometrial and squamous non-small cell lung carcinoma (NCT03366480). Previously, we showed that induces TRIB3 pseudokinase expression, resulting the inhibition of AKT-MTORC1 axis macroautophagy/autophagy-mediated cancer death. However, precise molecular determinants involved cytotoxic autophagy caused by remained unclear. Using wide range biochemical...
Lung cancer is one of the most aggressive tumours with very low life expectancy. Altered microRNA expression found in human because it involved tumour growth, progression and metastasis. In this study, we analysed 47 lung biopsies. Among downregulated microRNAs focussed on miR-99a characterisation. vitro experiments showed that decreases proliferation H1650, H1975 H1299 cells causing cell cycle arrest apoptosis. We identified two novel proteins, E2F2 (E2F transcription factor 2) EMR2...
J. Neurochem . (2008) 104, 1599–1612. Abstract Mitochondrial alterations have been associated with the cytotoxic effect of 6‐hydroxydopamine (6‐OHDA), a widely used toxin to study Parkinson’s disease. In previous work, we demonstrated that 6‐OHDA increases mitochondrial membrane permeability leading cytochrome c release, but precise mechanisms involved in this process remain unknown. Herein studied mechanism increased SH‐SY5Y neuroblastoma cells response 6‐OHDA. Cytochrome release induced by...
Malonate, an inhibitor of mitochondrial complex II, is a widely used toxin to study neurodegeneration in Huntington9s disease and ischemic stroke. We have shown previously that malonate increased reactive oxygen species (ROS) production human SH-SY5Y neuroblastoma cells, leading oxidative stress, cytochrome <i>c</i> release, apoptotic cell death. Expression green fluorescent protein-Bax fusion protein cells demonstrated Bax redistribution from the cytosol mitochondria after 12 24 h treatment...
Abstract Fas ligand (FasL)‐receptor system plays an essential role in regulating cell death the developing nervous system, and it has been implicated neurodegenerative inflammatory responses CNS. Lifeguard (LFG) is a protein highly expressed hippocampus cerebellum, shows particularly interesting regulation by being up‐regulated during postnatal development adult. We show that over‐expression of LFG protected cortical neurons from FasL‐induced apoptosis decreased caspase‐activation. Reduction...
Death receptors (DRs) and their ligands are expressed in developing nervous system. However, neurons generally resistant to death induction through DRs rather activation promotes neuronal outgrowth branching. These results suppose the existence of antagonists Fas apoptosis inhibitory molecule (FAIM S ) was first identified as a antagonist B-cells. Soon after, longer alternative spliced isoform with unknown function named FAIM L . is widely expressed, including system, we have shown...
Several reports have demonstrated a role for aberrant NOTCH signaling in melanoma genesis and progression, prompting us to explore if targeting this pathway is valid therapeutic approach against melanoma. We targeted using RO4929097, novel inhibitor of gamma secretase, which key component the enzymatic complex that cleaves activates NOTCH. The effects RO4929097 on oncogenic stem cell properties panel lines were tested both vitro vivo, xenograft models. In human primary lines, decreased...
Fas apoptosis inhibitory molecule (FAIM) is a protein identified as an antagonist of Fas-induced cell death. We show that FAIM overexpression fails to rescue neurons from trophic factor deprivation, but exerts marked neurite growth–promoting action in different neuronal systems. Whereas greatly enhanced outgrowth PC12 cells and sympathetic grown with nerve growth (NGF), reduction endogenous levels by RNAi decreased these cells. promoted NF-κB activation, blocking this activation using...
Reelin binds to very low–density lipoprotein receptor and apolipoprotein E 2, thereby inducing mDab1 phosphorylation activation of the phosphatidylinositide 3 kinase (PI3K) pathway. Here we demonstrate that activates mitogen-activated protein kinase/extracellular signal-regulated (ERK) pathway, which leads Erk1/2 proteins. The inhibition Src family kinases (SFK) blocked Reelin-dependent activation. This was also shown in neuronal cultures from mDab1-deficient mice. Although rat sarcoma viral...