A5070430951- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Protein purification and stability
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Virus-based gene therapy research
- Chemical Synthesis and Analysis
- Animal Nutrition and Physiology
- Healthcare Regulation
- Contact Dermatitis and Allergies
- Influenza Virus Research Studies
- Bacteriophages and microbial interactions
- Education during COVID-19 pandemic
- Antibiotics Pharmacokinetics and Efficacy
- Protein Hydrolysis and Bioactive Peptides
- Veterinary Oncology Research
- Social and Political Issues
- Healthcare during COVID-19 Pandemic
- Pharmaceutical studies and practices
- Nanopore and Nanochannel Transport Studies
- Agriculture and Biological Studies
Instituto de Soldadura e Qualidade
2025
University of Lisbon
2024
AB Vista (United Kingdom)
2024
University of Beira Interior
2016-2023
Polydoro Ernani de São Thiago University Hospital
2022
Université de Lille
2022
O uso inadequado de antibióticos na população pediátrica tem sido um problema recorrente, impulsionado pela prescrição excessiva para infecções virais e falta critérios diagnósticos bem definidos em alguns contextos clínicos. A exposição precoce desnecessária a esses fármacos pode comprometer o equilíbrio da microbiota intestinal, predispondo alterações imunológicas metabólicas, além contribuir crescente resistência bacteriana, fenômeno que compromete eficácia terapêutica dos...
AS1411 is a G-quadruplex (G4) aptamer that binds tightly to nucleolin (NCL) on the cell surface and has shown strong anticancer effects. However, this highly polymorphic, presenting different types of G4s, which may hinder its preclinical application. Several modifications have been made decrease polymorphism aptamer. In work, we designed six derivatives by substituting guanine with thymine in central linker modifying number thymines either itself and/or at both ends sequence. The G4...
We have designed AS1411-N6, a derivative of the nucleolin (NCL)-binding aptamer AS1411, by adding six nucleotides to 5′-end that are complementary at 3′-end forcing it into stem-loop structure. evaluated several biophysical techniques if AS1411-N6 can adopt one or more conformations, which allows NCL binding. found decrease polymorphism G-quadruplex (G4)-forming sequences comparing AS1411 and G4 formation in presence K+ promotes duplex folding. also studied binding properties ligands TMPyP4,...
G-rich aptamers such as AS1411 are small oligonucleotides that present several benefits comparatively to monoclonal antibodies, since they easier manufacture and store, have size do not stimulate an immune response. We analyzed AT11-B1, a modified sequence of AT11 (itself version AS1411), in which one thymine was removed from the bulge region. studied G-quadruplex (G4) formation/stabilization using PhenDC3, PDS, BRACO-19, TMPyP4 360A ligands by different biophysical techniques, namely...
Using a molecular dynamics approach, the study of interaction between six different known ligands and predicted pre-miRNA 149 RNA G-quadruplex (rG4) structure is reported. The stabilization rG4 structures formed within stem-loop regions using small an attractive anticancer strategy. Particularly, miRNA-149 upregulated in variety cancers such as prostate cancer therefore potential target for drug development. results show that C8 PhenDC3 interact with via stacking interactions end G-quartets....
Let-7e precursor microRNA has the potential to adopt a G-quadruplex (rG4) structure and recently, its roles in oncology have been focus of much attention, as it is now known that let-7e pre-miRNA frequently dysregulated cancers. Therefore, crucial unveil fully characterize ability rG4 structure, which could be stabilized or destabilized by small molecules proteins such nucleolin, protein deeply associated with miRNA biogenesis. Herein, combining set different methods circular dichroism (CD),...
One of the most significant challenges in capturing and detecting biomarkers is choice an appropriate biomolecular receptor. Recently, RNA G-quadruplexes emerged as plausible receptors due to their ability recognize with high-affinity proteins. Herein, we have unveiled characterized capability precursor microRNA 149 form a G-quadruplex structure determined role that some ligands may its folding binding capacity nucleolin. The formation was induced by K+ ions stabilized ligands, demonstrated...
In this work we explore the structure of a G-rich DNA aptamer termed AT11-L2 (TGGTGGTGGTTGTTGTTGGTGGTGGTGGT; derivative AT11) by evaluating formation and stability G-quadruplex (G4) conformation under different experimental conditions such as KCl concentration, temperature, upon binding with variety G4 ligands (360A, BRACO-19, PDS, PhenDC3, TMPyP4). We also determined whether nucleolin (NCL) can be target G4. Firstly, assessed circular dichroism, UV NMR spectroscopies AT11-L2. observed that,...
The fast spread of SARS-CoV-2 has led to a global pandemic, calling for and accurate assays allow infection diagnosis prevention transmission. We aimed develop molecular beacon (MB)-based detection assay SARS-CoV-2, designed detect the ORF1ab S genes, proposing two-stage COVID-19 testing strategy. novelty this work lies in design optimization two MBs namely, concentration, fluorescence plateaus hybridization, reaction temperature real-time results. also identify putative G-quadruplex (G4)...
The structural determinants of the interaction G-quadruplex (G4) motif found in precursor miRNA 149 (rG4) with acridine orange derivative C8 , a G4 ligand stabilizer possessing anticancer activity, and protein nucleolin (overexpressed cancer cells) were investigated by Nuclear Magnetic Resonance (NMR) spectroscopy. For rG4/C8 complex, results revealed strong stabilizing between aromatic core iodinated ring rG4 structure. NMR study also different patterns complex. In absence ligand,...
The selective isolation of G-quadruplex (G4) using affinity ligands that bind tightly and selectively is a valuable strategy for discovering new G4 binders the separation from duplexes or discrimination structures.
Abstract Phytase hyper-dosing is a recent concept in nutrition based on the use of high doses enzyme with growth promoting effect. Supplementary release inosotol, rapid and strong destruction phytic acid other non-phosphoric effects are proposed mechanisms for positive results found pig growth. The hypothesis this would be to accelerate phytate improving feed conversion ratio (FCR). A second that increased may generate decreased concentrations cortisol, because inositol has insulin-like...