A5033912684- Autoimmune and Inflammatory Disorders Research
- Systemic Lupus Erythematosus Research
- Rheumatoid Arthritis Research and Therapies
- Renal Diseases and Glomerulopathies
- Immunodeficiency and Autoimmune Disorders
- Advanced biosensing and bioanalysis techniques
- Bone Metabolism and Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- SARS-CoV-2 detection and testing
- Musculoskeletal synovial abnormalities and treatments
- Cytokine Signaling Pathways and Interactions
- Bone health and treatments
- Orthopedic Infections and Treatments
- Biosensors and Analytical Detection
- Monoclonal and Polyclonal Antibodies Research
- Orthopaedic implants and arthroplasty
- Cancer Diagnosis and Treatment
- Adolescent and Pediatric Healthcare
- Spondyloarthritis Studies and Treatments
- T-cell and B-cell Immunology
- Pharmacological Effects of Medicinal Plants
- Sarcoidosis and Beryllium Toxicity Research
- Immune Cell Function and Interaction
- Natural Compounds in Disease Treatment
- Extracellular vesicles in disease
Centro Hospitalar Lisboa Norte
2017-2024
Hospital de Santa Maria
2017-2024
University of Lisbon
2017-2024
Instituto Gulbenkian de Ciência
2024
Administração Regional de Saúde de Lisboa e Vale do Tejo
2024
Instituto de Medicina Molecular João Lobo Antunes
2013-2024
All India Institute of Medical Sciences Raipur
2023
Third Affiliated Hospital of Sun Yat-sen University
2023
Sun Yat-sen University
2023
Cincinnati Children's Hospital Medical Center
2023
The osteolytic nature of bone metastasis results from a tumor-driven increased resorption. Bone remodeling is orchestrated by the molecular triad RANK-RANKL-OPG. This process dysregulated in metastases, mostly via induction RANKL tumor-derived factors. These factors increase expression RANKL, which induce osteoclast formation, function, and survival, thereby increasing RANK unexpectedly expressed cancer cells, activation RANKL-RANK pathway correlates with an invasive phenotype. To...
In this study, we optimized the dissociation of synovial tissue biopsies for single-cell omics studies and created a atlas human synovium in inflammatory arthritis. The protocol allowed consistent isolation highly viable cells from tiny fresh biopsies, minimizing biopsy drop-out rate. scRNA-seq contained over 100,000 unsorted 25 tissues affected by arthritis, including 16 structural, 11 lymphoid, 15 myeloid cell clusters. This map expanded diversity types/states, detected neutrophils,...
We present a European multicenter study, comparing safety data and patient-reported outcomes (PRO) from patients undergoing synovial biopsy using ultrasound-guided needle (US-NB), portal forceps (US-P&F) or arthroscopic-guided (AG) procedures. To describe PRO on joint indices of pain, stiffness swelling before after biopsy, procedural discomfort, status compared with willingness to undergo second for each technique compare the three techniques. evaluate impact corticosteroid therapy as part...
Objective Rheumatoid arthritis (RA) is characterised by chronic inflammation leading to articular bone and cartilage damage. Despite recent progress in RA management, adverse effects, lack of efficacy economic barriers treatment access still limit therapeutic success. Therefore, safer less expensive treatments that control resorption are needed. We have previously shown celastrol a candidate for treatment. observed it inhibits both interleukin (IL)-1β tumor necrosis factor (TNF) vitro, has...
The main goal of this study was to characterise the frequency and phenotype B, T follicular helper (Tfh) regulatory (Tfr) cells in peripheral blood cytokine environment present circulation children with extended oligoarticular juvenile idiopathic arthritis (extended oligo JIA) polyarticular JIA (poly when compared healthy controls, persistent (persistent adult patients.Blood samples were collected from 105 patients (children adults) 50 age-matched individuals. Tfh Tfr evaluated by flow...
The fast spread of SARS-CoV-2 has led to a global pandemic, calling for and accurate assays allow infection diagnosis prevention transmission. We aimed develop molecular beacon (MB)-based detection assay SARS-CoV-2, designed detect the ORF1ab S genes, proposing two-stage COVID-19 testing strategy. novelty this work lies in design optimization two MBs namely, concentration, fluorescence plateaus hybridization, reaction temperature real-time results. also identify putative G-quadruplex (G4)...
Background Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children 1 . Our group has recently demonstrated that extended oligoarticular (eoJIA) and polyarticular JIA (pJIA) mostly evolve to a rheumatoid (RA) like phenotype adulthood 2 Disturbances B cells, T follicular helper (Tfh) regulatory (Tfr) cell immune responses are associated with pathogenesis of RA 3,4 , but their exact role development not entirely known. Objectives The main goal this study was...
<h3>Background:</h3> Remission is nowadays an attainable target for most rheumatoid arthritis (RA) patients, but there a lack of accurate remission markers selecting patients treatment taper. Although ultrasound (US) proved to detect minimal disease activity even in classified clinical remission, other methods, such as contrast-enhanced (CEUS), might be more sensitive and thus candidates act "true" practice. <h3>Objectives:</h3> To test CEUS useful tool inflammation RA sustained correlate it...
Objective The study aimed to explore the utility of contrast-enhanced ultrasound (CEUS) as a tool for detecting minimal inflammation in rheumatoid arthritis (RA) patients sustained remission (SR) and correlate findings with Disease Activity Score 28 (DAS28) status scores various (US) scores. Patients methods Thirty RA SR (minimum 6 months), 12 active disease, 10 healthy controls were included. Clinical evaluations US assessments performed, including grayscale (GSUS), power Doppler (PDUS),...
<h3>Background:</h3> Inflammatory arthritides and other rheumatic diseases often present with clinical arthritis, leading to a significant personal societal burden. Early arthritis clinics have been established worldwide identify treat patients within window of opportunity, expecting improve outcomes. <h3>Objectives:</h3> To describe the organization our early clinic, which manages untreated inflammatory less than 12 months symptoms. <h3>Methods:</h3> We included followed-up in clinic from...
<h3>Background:</h3> Early inflammatory arthritis is a condition that may evolve to several known rheumatic diseases, such as rheumatoid (RA). Alterations in JAK-STAT signaling pathway have been documented immune-mediated disorders RA. We hypothesize JAK- STAT key chronic onset and its early inhibition might major effect on allowing lasting disease control. <h3>Objectives:</h3> aimed characterize peripheral blood leukocyte populations of untreated patients (with <1 year symptom duration)...
<h3>Background:</h3> Rheumatoid arthritis (RA) is a chronic, systemic, immune-mediated inflammatory disease that mainly targets the joints. The pathogenesis of RA involves dysregulations in immune pathways such as JAK-STAT signaling pathway. JAK inhibitors (JAKi) are class modifying anti-rheumatic drugs approved for treatment. <h3>Objectives:</h3> To compare in-vitro cellular effect two JAKi, tofacitinib and upadacitinib, peripheral blood leukocytes from patients with untreated early...
The fast spread of SARS-CoV-2 has led to a global pandemic, calling for and accurate assays allow infection diagnosis prevention transmission. We aimed develop molecular beacon (MB)-based detection assay SARS-CoV-2, designed to, detect the ORF1ab S genes, proposing two-stage COVID-19 testing strategy, using MBs presence target amplicons by fluorescence analysis. Two were designed, optimized in terms concentration, plateaus hybridization, reaction temperature best real-time results. A total...
<h3>Background</h3> Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children. Our group has recently demonstrated that extended oligoarticular and polyarticular JIA mostly evolve to a rheumatoid (RA)-like phenotype adulthood. Disturbances B cells, T follicular helper (Tfh) regulatory (Tfr) cell immune responses are associated with RA pathogenesis, but their exact role development poorly understood. <h3>Objectives</h3> The main goal of this study was characterize...
<h3>Background</h3> Disturbances in B cell immune responses have been implicated rheumatoid arthritis (RA) pathogenesis since the first weeks of RA development. However, mechanisms responsible for these alterations remain unclear. Follicular helper T (Tfh) cells are crucial maturation, activation and class-switching as well germinal center (GC) formation, whereas follicular regulatory (Tfr) can modulate GC reaction by suppressing Tfh cells. <h3>Objectives</h3> The main goal this study was to...
<h3>Background</h3> Janus kinase inhibitors (JAKi) are a new therapeutic class approved for the treatment of chronic arthritis. JAKi suppress activity STAT tyrosine kinases, interfering with signaling pathway which is critical immune cell proliferation, survival and differentiation. Our group has demonstrated that early JAKi, in animal models, abrogates disease prevent bone damage. We hypothesize JAK-STAT key to arthritis onset its inhibition might have major effect on control....
<h3>Background</h3> Our group has recently described that the majority of polyarticular juvenile idiopathic arthritis (pJIA) and a large fraction extended oligoarticular JIA (oJIA) patients fulfil classification criteria for rheumatoid (RA) in adulthood. B cells play several important roles RA pathogenesis, but it is still unclear if pattern cell involvement pJIA oJIA follows what been adults with RA. <h3>Objectives</h3> The main goal this study was to characterise peripheral blood phenotype...
<h3>Background</h3> Synovial tissue analysis could provide a step towards personalised medicine in daily clinical practice for patients with inflammatory arthritis. However systematic reports comparing the patient's perspective when undergoing synovial biopsy according to different sampling techniques is currently missing from literature. We here present multicenter study across Europe, patient reported outcomes (PRO) and safety data procedures using either ultrasound guided needle (US-NB),...
<h3>Background</h3> The majority of polyarticular JIA (pJIA) and a large fraction extended oligoarticular (oJIA) patients fulfil classification criteria for rheumatoid arthritis (RA) in adulthood. B cells play several important roles RA pathogenesis, but it is still unclear if the pattern cell involvement pJIA oJIA follows what has been described adults with RA. <h3>Objectives</h3> main goal this study was to determine concentration cytokines potentially relevant activation serum from...
Background: Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children. The majority of polyarticular JIA (pJIA) and a large fraction extended oligoarticular (oJIA) patients fulfil classification criteria for rheumatoid (RA) adulthood. B-cells play several important roles RA pathogenesis, but it still unclear if pattern B-cell involvement pJIA oJIA follows what has been described adults with RA. Objectives: main goal this study was to determine concentration...