A5012975663- Rheumatoid Arthritis Research and Therapies
- Bone Metabolism and Diseases
- Cytokine Signaling Pathways and Interactions
- Natural Compounds in Disease Treatment
- Systemic Lupus Erythematosus Research
- Bone and Joint Diseases
- Glioma Diagnosis and Treatment
- Autoimmune and Inflammatory Disorders Research
- Bone health and osteoporosis research
- Brain Metastases and Treatment
- Cancer Genomics and Diagnostics
- Nanoplatforms for cancer theranostics
- Lung Cancer Research Studies
- Bone health and treatments
- Spondyloarthritis Studies and Treatments
- Chronic Lymphocytic Leukemia Research
- Inflammasome and immune disorders
- HER2/EGFR in Cancer Research
- Nanoparticle-Based Drug Delivery
- Immune cells in cancer
- Genomic variations and chromosomal abnormalities
- Pharmacological Effects of Medicinal Plants
- Genomics and Rare Diseases
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
University of Lisbon
2014-2024
European Bioinformatics Institute
2024
Royal National Orthopaedic Hospital
2024
Genomics England
2024
Boston Children's Hospital
2024
University College London
2024
Hospital de Egas Moniz
2010-2023
Instituto de Medicina Molecular João Lobo Antunes
2010-2017
Amsterdam UMC Location University of Amsterdam
2014
Instituto de Medicina Tropical
2010-2013
Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by sustained synovitis. Recently, several studies have proposed neutrophils and Th17 cells as key players in the onset perpetuation of this disease. The main goal work was to determine whether cytokines driving neutrophil activation are dysregulated very early rheumatoid patients with less than 6 weeks duration before treatment (VERA). Cytokines related were quantified serum VERA established RA compared...
B cells play an important role in the perpetuation of RA, particularly as autoantibody-producing cells. The ICs that further develop deposit joints and aggravate inflammatory process. However, B-cell contribution very early stage disease remains unknown. main goal this work was to determine concentration cytokines potentially relevant for activation serum from polyarthritis patients, with <6 weeks duration, who latter on evolved into RA (VERA).A proliferation-inducing ligand (APRIL),...
Abstract Telomere fusions (TFs) can trigger the accumulation of oncogenic alterations leading to malignant transformation and drug resistance. Despite their relevance in tumour evolution, our understanding patterns consequences TFs human cancers remains limited. Here, we characterize rates spectrum somatic across >30 cancer types using whole-genome sequencing data. are pervasive tumours with varying markedly within types. In addition end-to-end fusions, find that mechanistically link...
Abstract Background Intratumoral heterogeneity is crucially involved in metastasis, resistance to therapy, and cancer relapse. Amplifications of the proto-oncogene MYC display notable at single-cell level are associated with a particularly dismal prognosis high-risk medulloblastomas (MBs). The aim this study was establish relevance interclonal cross-talk between MYC-driven non-MYC-driven MB cells. Methods We used fluorescence situ hybridization, transcriptomics, immunohistochemistry, vitro...
Accurate detection of somatic structural variants (SVs) and copy number aberrations (SCNAs) is critical to inform the diagnosis treatment human cancers. Here, we describe SAVANA, a computationally efficient algorithm designed for joint analysis SVs, SCNAs, tumour purity ploidy using long-read sequencing data. SAVANA relies on machine learning distinguish true SVs from artefacts provide prediction errors individual SVs. Using high-depth Illumina nanopore whole-genome data 99 tumours matched...
Background Rheumatoid arthritis (RA) is a chronic immune-mediated inflammatory disease characterized by cellular infiltration into the joints, hyperproliferation of synovial cells and bone damage. Available treatments for RA only induce remission in around 30% patients, have important adverse effects its use limited their high cost. Therefore, compounds that can control arthritis, with an acceptable safety profile low production costs are still unmet need. We shown, vitro, celastrol inhibits...
The main goal of this work was to analyse how treatment intervention with tofacitinib prevents the early disturbances bone structure and mechanics in rat model adjuvant-induced arthritis. This is first study access impact on skeletal effects inflammation.Fifty Wistar rats arthritis were randomly housed experimental groups, as follows: non-arthritic healthy group (n = 20); arthritic non-treated 10 animals undergoing treatment. Rats monitored during 22 days after disease induction for...
Background . We have previously reported a continuous activation of caspase-1 and increased interleukin (IL)-1 β levels in early rheumatoid arthritis (RA). These observations raised the hypothesis that drugs targeting IL-1 pathway, addition to tumour necrosis factor (TNF), may be particularly effective for RA treatment. recently identified gambogic acid as promising therapeutic candidate simultaneously block TNF secretion. Our main goal here was investigate whether administration able...
Abstract Introduction In this study we used a mice model of chronic arthritis to evaluate if bone fragility induced by inflammation is associated with an imbalance in turnover and also disorganization the type I collagen network. Methods Serum, vertebrae femur bones were collected from eight-month-old polyarthritis SKG controls. Strength femoral was evaluated using three-point bending tests density assessed pycnometer. Bone markers carboxy-terminal cross-linking telopeptides (CTX-I)...
Dissemination of cancer cells from primary tumors to the brain occurs in many patients, increasing morbidity and death. There is an unmet medical need develop translational platforms evaluate therapeutic responses. Toward this goal, we established a library 23 patient-derived xenografts (PDXs) metastases (BMs) eight distinct tumors. In vivo tumor formation correlates with patients' poor survival. Mouse subcutaneous spontaneous intracardiac PDXs increase dissemination CNS, both models...
Objective Rheumatoid arthritis (RA) is characterised by chronic inflammation leading to articular bone and cartilage damage. Despite recent progress in RA management, adverse effects, lack of efficacy economic barriers treatment access still limit therapeutic success. Therefore, safer less expensive treatments that control resorption are needed. We have previously shown celastrol a candidate for treatment. observed it inhibits both interleukin (IL)-1β tumor necrosis factor (TNF) vitro, has...
B cells play an important role in the perpetuation of rheumatoid arthritis (RA), particularly as autoantibody producing cells. The immune complexes that further develop deposit joints and aggravate inflammatory process. However, contribution very early stage disease remains unknown.
Background: We demonstrated that celastrol has significant anti-inflammatory and bone protective effects when administered via intraperitoneal route. For further preclinical evaluation an effective oral administration of is crucial. Here we aimed to study the therapeutic dose range for its administration. Methods: Celastrol (1-25µg/g/day, N=5/group) was administrated orally female AIA rats after 8 days disease induction a period 14-days. A group healthy (N=8) arthritic (N=15) gender...
Background We have previously found in the chronic SKG mouse model of arthritis that long standing (5 and 8 months) inflammation directly leads to high collagen bone turnover, disorganization network, disturbed microstructure degradation biomechanical properties. The main goal present work was study effects first days inflammatory process on microarchitecture mechanical properties bone. Methods Twenty eight Wistar adjuvant-induced (AIA) rats were monitored during 22 after disease induction...
<title>Abstract</title> Accurate detection of somatic structural variants (SVs) and copy number aberrations (SCNAs) is critical to inform the diagnosis treatment human cancers. Here, we describe SAVANA, a computationally efficient algorithm designed for joint analysis SVs, SCNAs, tumour purity ploidy using long-read sequencing data. SAVANA relies on machine learning distinguish true SVs from artefacts provide prediction errors individual SVs. Using high-depth Illumina nanopore whole-genome...