A5067843845- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Rheumatoid Arthritis Research and Therapies
- Immune Response and Inflammation
- Inflammasome and immune disorders
- Natural Compounds in Disease Treatment
- Plant Virus Research Studies
- interferon and immune responses
- Pineapple and bromelain studies
- Hops Chemistry and Applications
- Biological Activity of Diterpenoids and Biflavonoids
- Transgenic Plants and Applications
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Bone Metabolism and Diseases
- Mosquito-borne diseases and control
- Sepsis Diagnosis and Treatment
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Chromosomal and Genetic Variations
- Nosocomial Infections in ICU
- Alzheimer's disease research and treatments
- Drug Transport and Resistance Mechanisms
- Plant and Fungal Interactions Research
- Natural Compound Pharmacology Studies
Instituto Gulbenkian de Ciência
2016-2024
University of Lisbon
2009-2014
Escola Secundária Quinta do Marquês
2008
Alpha-Synuclein (aSyn) misfolding and aggregation is common in several neurodegenerative diseases, including Parkinson's disease dementia with Lewy bodies, which are known as synucleinopathies. Accumulating evidence suggests that secretion cell-to-cell trafficking of pathological forms aSyn may explain the typical patterns progression. However, molecular mechanisms controlling spreading pathology still elusive. In order to obtain unbiased information about regulators oligomerization, we...
Background . We have previously reported a continuous activation of caspase-1 and increased interleukin (IL)-1 β levels in early rheumatoid arthritis (RA). These observations raised the hypothesis that drugs targeting IL-1 pathway, addition to tumour necrosis factor (TNF), may be particularly effective for RA treatment. recently identified gambogic acid as promising therapeutic candidate simultaneously block TNF secretion. Our main goal here was investigate whether administration able...
Plasmodium sporozoites are transmitted by Anopheles mosquitoes and infect hepatocytes, where a single sporozoite replicates into thousands of merozoites inside parasitophorous vacuole. The nature the Plasmodium-host cell interface, as well interactions occurring between these two organisms, remains largely unknown. Here we show that highly dynamic hepatocyte actin reorganization events occur around developing berghei parasites human hepatoma cells. Actin is most prominent 10 to 16 hours post...
Sepsis is a leading cause of death in Intensive Care Units. Despite its prevalence, sepsis remains insufficiently understood, with no substantial qualitative improvements treatment the past decades. Immunomodulatory agents may hold promise, given significance TNF-α and IL-1β as mediators. This study examines immunomodulatory effects moxifloxacin, fluoroquinolone utilized clinical practice. THP1 cells were treated vitro either PBS or moxifloxacin subsequently challenged lipopolysaccharide (LPS)
The generation of diversity and plasticity transcriptional programs are key components effective vertebrate immune responses. role Alternative Splicing has been recognized, but it is underappreciated poorly understood as a critical mechanism for the regulation fine-tuning physiological Here we report loss-of-function phenotypes large collection genes known or predicted to be involved in splicing reaction identification 19 novel regulators IL-1β secretion response E. coli challenge THP-1...
<h3>Background</h3> We have previously reported an increase in interleukin (IL)-1β levels very recent onset arthritis. Therefore, molecular mechanisms that regulate IL-1β secretion, such as caspase-1 activation, might play a relevant role arthritis and progression. <h3>Objectives</h3> Our main goal was to evaluate state of activation early polyarthritis established rheumatoid (RA) patients. <h3>Materials methods</h3> Blood samples were collected from 12 untreated patients, RA patients 10...
We have previously reported an increase in interleukin (IL)-1β levels and a continuous activation of caspase-1 rheumatoid arthritis (RA) patients the early phase disease. This result suggests that drugs targeting IL-1β regulatory pathways, addition to tumor necrosis factor (TNF), may constitute promising therapeutic agents RA. recently used THP-1 macrophage-like cell line screen 2320 compounds for those which down-regulate TNF secretion. Gambogic acid, celastrol pristimerin were three most...
Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint destruction. We have previously reported an increase in interleukin (IL)-1β levels the serum of very early RA and synovial fluid (SF) patients. Therefore, molecular mechanisms that regulate IL-1β activation, such as caspase-1, might play relevant role potential therapeutic targets RA.
In this work we have studied the cycle of PDV and PNRSV, along year in two almond producing regions (Algarve Tras-os-Montes) plus a third one, middle Portugal. The data were collected always from same trees each region ('Boa Casta' 'Bonita de Sao Bras'). We also virus distribution tissue, using situ RT-PCR, found that flower buds, appears all meristematic cells, located mostly around nucleus forming ring-like structure. Interestingly, was inside pollen grains, again nuclear region. These are...