A5002278454- MicroRNA in disease regulation
- Brain Metastases and Treatment
- Circular RNAs in diseases
- Lung Cancer Research Studies
- Glioma Diagnosis and Treatment
- Chromatin Remodeling and Cancer
- RNA Interference and Gene Delivery
- Polyomavirus and related diseases
- Advanced biosensing and bioanalysis techniques
- Wnt/β-catenin signaling in development and cancer
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Axon Guidance and Neuronal Signaling
- Kruppel-like factors research
- Alzheimer's disease research and treatments
- Chemical Reactions and Isotopes
- Lung Cancer Treatments and Mutations
- Autophagy in Disease and Therapy
- T-cell and Retrovirus Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Epigenetics and DNA Methylation
- HER2/EGFR in Cancer Research
- Cancer Treatment and Pharmacology
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
University of Lisbon
2018-2023
Hospital de Egas Moniz
2022
Instituto de Medicina Molecular João Lobo Antunes
2018
University of Coimbra
2013-2015
MicroRNAs (miRNAs) have emerged as a class of small, endogenous, regulatory RNAs that exhibit the ability to epigenetically modulate translation mRNAs into proteins. This feature enables them control cell phenotypes and, consequently, modify function in disease context. The role inflammatory miRNAs Alzheimer's (AD) and their glia responses are now beginning be explored. In this study, we propose disclose functional miR-155, one most well studied immune-related AD-associated neuroinflammatory...
The present work aimed at the development and application of a lipid-based nanocarrier for targeted delivery nucleic acids to glioblastoma (GBM). For this purpose, chlorotoxin (CTX), peptide reported bind selectively glioma cells while showing no affinity non-neoplastic cells, was covalently coupled liposomes encapsulating antisense oligonucleotides (asOs) or small interfering RNAs (siRNAs). resulting nanoparticles, designated CTX-coupled stable acid lipid particles (SNALPs), exhibited...
Glioblastoma (GBM) is a universally fatal brain cancer, for which novel therapies targeting specific underlying oncogenic events are urgently needed. While the WNT pathway has been shown to be frequently activated in GBM, constituting potential therapeutic target, relevance of WNT6, an activator this pathway, remains unknown. Methods: WNT6 protein and mRNA levels were evaluated GBM. silenced or overexpressed GBM cells assess functional effects vitro vivo. Phospho-kinase arrays TCF/LEF...
Abstract Background Intratumoral heterogeneity is crucially involved in metastasis, resistance to therapy, and cancer relapse. Amplifications of the proto-oncogene MYC display notable at single-cell level are associated with a particularly dismal prognosis high-risk medulloblastomas (MBs). The aim this study was establish relevance interclonal cross-talk between MYC-driven non-MYC-driven MB cells. Methods We used fluorescence situ hybridization, transcriptomics, immunohistochemistry, vitro...
Dissemination of cancer cells from primary tumors to the brain occurs in many patients, increasing morbidity and death. There is an unmet medical need develop translational platforms evaluate therapeutic responses. Toward this goal, we established a library 23 patient-derived xenografts (PDXs) metastases (BMs) eight distinct tumors. In vivo tumor formation correlates with patients' poor survival. Mouse subcutaneous spontaneous intracardiac PDXs increase dissemination CNS, both models...
Glioblastoma (GBM) is the most common and malignant primary brain tumor in adults. The prognosis of patients very poor, with a median overall survival ~ 15 months after diagnosis. Cadherin-3 (also known as P-cadherin), cell-cell adhesion molecule encoded by CDH3 gene, deregulated several cancer types, but its relevance GBM unknown. In this study, we investigated functional roles, associated molecular signatures, prognostic value CDH3/P-cadherin highly tumor. mRNA protein levels were...
Abstract Background Despite current improvements in systemic cancer treatment, brain metastases (BM) remain incurable, and there is an unmet clinical need for effective targeted therapies. Methods Here, we sought common molecular events metastatic disease. RNA sequencing of thirty human BM identified the upregulation UBE2C, a gene that ensures correct transition from metaphase to anaphase, across different primary tumor origins. Results Tissue microarray analysis independent patient cohort...
Primary brain tumors are a leading cause of cancer-related death in children, and medulloblastoma is the most common malignant pediatric tumor. The current molecular characterization mainly based on protein-coding genes, while little known about involvement long non-coding RNAs (lncRNAs). This study aimed to elucidate role lncRNA OTX2-AS1 medulloblastoma.Analyses DNA copy number alterations, methylation profiles, gene expression data were used characterize alterations tissue samples. In...
Abstract Atypical teratoid/rhabdoid tumor (AT/RT) is a highly malignant brain in infants that characterized by loss of nuclear expression SMARCB1 or SMARCA4 proteins. Recent studies show AT/RTs comprise three molecular subgroups, namely AT/RT-TYR, AT/RT-MYC and AT/RT-SHH. The subgroups distinct patterns genes involved ciliogenesis, however, little known about the functional roles primary cilia biology AT/RT. Here, we are present across all AT/RT with specific enrichment AT/RT-TYR patient...
Triple-negative breast cancer (TNBC) is a devastating BC subtype. Its aggressiveness, allied to the lack of well-defined molecular targets, usually culminates in appearance metastases that account for poor prognosis, particularly when they develop brain. Nevertheless, TNBC has been associated with epidermal growth factor receptor (EGFR) overexpression, leading downstream phosphoinositide 3-kinase (PI3K) signaling activation. We aimed unravel novel drug candidates treatment based on EGFR...
ABSTRACT Purpose Dissemination of cancer cells from primary tumors to the brain is observed in great majority patients, contributing increased morbidity and being main cause death. Most mechanistic preclinical studies have relied on aggressive cell lines, which fail represent tumor heterogeneity are unsuitable validate therapies due fast progression vivo . Experimental design We established a unique library subcutaneous intracardiac patient-derived xenografts (PDXs) metastases (BMs) eight...
INTRODUCTION: Medulloblastoma is the most common malignant brain tumor of childhood and a genetically heterogeneous disease. Amongst all medulloblastoma subgroups, MYC-amplified medulloblastomas are associated with particularly poor prognosis as they commonly metastatic resistant to standard therapy. However, MYC amplification restricted cellular subpopulation within tumors. The underlying mechanisms mediating this heterogeneity remain poorly understood. HYPOTHESIS: We hypothesize that...
Atypical teratoid/rhabdoid tumors (AT/RT) are among the most common malignant brain in infants. Comprehensive genomic studies revealed three distinct molecular subgroups (AT/RT-TYR, -MYC and -SHH). As primary cilia (PC) have already been shown to play a pivotal role other tumor entities, we aimed characterize distribution of PC across AT/RT target ciliogenesis these with dismal prognosis. We performed immunofluorescence detect sections cell lines. The functional was investigated vitro by...
Abstract Brain metastases (BMs) are a devastating complication of advanced cancers associated with poor prognosis. Contrarily to the current improvement in systemic therapies, BMs still incurable and one main causes death cancer patients. We analyzed from thirty patients various primary tumor origins by RNA sequencing identified upregulation UBE2C, gene involved correct transition metaphase anaphase. Using an independent cohort BMs, we demonstrated that high protein expression UBE2C was...
ABSTRACT Despite current improvements in systemic cancer treatment, brain metastases (BM) remain incurable, and there is an unmet clinical need for effective targeted therapies. Here, we sought common molecular events metastatic disease. RNA sequencing of thirty human BM identified the upregulation UBE2C , a gene that ensures correct transition from metaphase to anaphase, across different primary tumor origins. Tissue microarray analysis independent patient cohort revealed high expression...
Abstract The dissemination of cancer cells to the brain parenchyma (brain metastases - BMs) and leptomeninges (leptomeningeal – LD) are a late-stage complication systemic cancers, with poor survival. Despite advances in radiation chemotherapy, including intrathecal administration anticancer agents, these forms advanced incurable. Therefore, there is an unmet clinical need for novel effective therapies that target both parenchymal leptomeningeal disease. We analysed transcriptomic profile BMs...