A5000419083- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- Neuropeptides and Animal Physiology
- Sleep and Wakefulness Research
- Mitochondrial Function and Pathology
- Circadian rhythm and melatonin
- Lipid Membrane Structure and Behavior
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neural Engineering
- Ion Channels and Receptors
- Photoreceptor and optogenetics research
- Protein Kinase Regulation and GTPase Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- Regulation of Appetite and Obesity
- Invertebrate Immune Response Mechanisms
- Molecular Sensors and Ion Detection
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Adipose Tissue and Metabolism
- Insect Resistance and Genetics
- Nerve injury and regeneration
- bioluminescence and chemiluminescence research
- Sleep and related disorders
University of Helsinki
2011-2022
Institute for Biomedicine
2012
University of Eastern Finland
2001-2010
Uppsala University
1997-2006
Kuopio University Hospital
1997-2005
University of Dundee
1980-2005
Ninewells Hospital
1980-2005
University of Ljubljana
2000
Université Libre de Bruxelles
2000
Åbo Akademi University
1988-1997
Local extracellular acidification has been demonstrated at sites of ischemia and inflammation. IL-1β is one the key proinflammatory cytokines, thus, its synthesis secretion are tightly regulated. The NLRP3 (nucleotide-binding domain leucine-rich repeat containing family, pyrin 3) inflammasome complex, assembled in response to microbial components or endogenous danger signals, triggers caspase-1-mediated maturation IL-1β. In this study, we explored whether acidic environment sensed by immune...
Fragile X syndrome, a common form of inherited mental retardation, is caused by the absence fragile retardation protein (FMRP) due to mutation in FMR1 gene. We investigated differentiation neural stem cells generated from brains fmr1- knockout (KO) mice and postmortem tissue fetus. Mouse human FMRP-deficient neurospheres more TuJ1-positive (3-fold 5-fold, respectively) than control normal mouse brains, these showed morphological alterations with fewer shorter neurites smaller cell body...
In this study, we have compared the abilities of orexin-A and orexin-B variants to activate different Ca<sup>2+</sup>responses (influx release) in human OX<sub>1</sub> OX<sub>2</sub> receptor- expressing Chinese hamster ovary cells. Responses mediated by activation both receptor subtypes with either or -B were primarily dependent on extracellular Ca<sup>2+</sup>, suggesting similar Ca<sup>2+</sup>influx as previously shown for OX<sub>1</sub>receptors. Amino acid-wise truncation reduced its...
Brain-derived neurotrophic factor (BDNF) signaling through its receptor TRKB modulates survival, differentiation, and activity of neurons. BDNF activates on the cell surface, which leads to initiation intracellular cascades different biological responses in Neuronal has been shown regulate levels plasma membrane neurons, but little is known about other factors affecting surface expression levels. We report here that regulates transfected or endogenously expressed full-length TRKB, depending...
In this study, the mechanism of OX(1) orexin receptors to regulate adenylyl cyclase activity when recombinantly expressed in Chinese hamster ovary cells was investigated. intact cells, stimulation with orexin-A led two responses, a weak (21%), high potency (EC(50) approximately 1 nm) inhibition and strong (4-fold), low = 300 stimulation. The reversed by pertussis toxin, suggesting involvement G(i/o) proteins. Orexin-B was, surprisingly, almost equally as potent elevating cAMP (pEC(50) 500...
The association of Ca2+ with isolated nerve endings (synaptosomes) is investigated and resolved into two components, that bound to the outer surface plasma membrane transported across membrane. When synaptosomes are added directly a Ca2+-containing medium, there an initial rapid uptake membrane, followed by slow proceeds for 20 min. phase not observed if initially pre-incubated in Ca2+-free medium. Rapid disruption reveals less than 3 nmol per mg synaptosomal protein can be ascribed...
Fragile X syndrome (FXS) is a common cause of inherited intellectual disability and well-characterized form autism spectrum disorder. As brain-derived neurotrophic factor (BDNF) implicated in the pathophysiology FXS we examined effects reduced BDNF expression on behavioral phenotype an animal model FXS, Fmr1 knockout (KO) mice, crossed with mice carrying deletion one copy Bdnf gene (Bdnf(+/-)). KO showed age-dependent alterations hippocampal that declined after age 4 months compared to...
Cerebellar granule neurons cultured with serum develop a mature neuronal phenotype, including stimulus-coupled release of glutamate, and depend on elevated potassium for survival. We find that cells undergo two phases cell death. By 6 d in vitro , 30–50% the present are dead; after this time remaining die. Elevated prevents only later phase death, whereas neurotrophins protect these against early Factors bind p75 NTR or TNF-R, members same receptor family, exhibit voltage-sensitive calcium...
Abstract: Membrane potentials of particles present in a sub‐cellular brain preparation, called synaptoneurosomes, have been monitored by measurement changes the absor‐bance cyanine dye, DiS‐C 2 ‐5. The membrane potential seems to be dependent on both Cl − and K + diffusion potentials, as judged from dependence ab‐sorbance equilibrium across presence Ba 2+ or when was replaced with gluconate. apparent high permeability preparation reduced pic‐rotoxin, finding suggesting endogenous activation...
Activation of OX1 orexin receptors heterologously expressed in Chinese hamster ovary (CHO) cells led to a rapid, strong, and long-lasting increase ERK phosphorylation (activation). Dissection the signal pathways using multiple inhibitors dominant-negative constructs indicated involvement Ras, protein kinase C, phosphoinositide-3-kinase, Src. Most interestingly, Ca2+ influx appeared central for response CHO cells, same was recombinant neuro-2a cultured rat striatal neurons. Detailed...
Ionophore A23187, a Ca2+/2H+ exchanger, has multiple effects on the function of isolated nerve endings (synaptosomes). (a) There is net uptake Ca2+, rate which increases with ionophore concentration. (b) When Ca2+ terminated by EGTA, induces an apparent efflux Ca2+. (c) The plasmamembrane potential, calculated from 86Rb+ distribution, decreases slowly upon addition ionophore. (d) Release [3H]noradrenaline induced consists rapid phase followed subsequent slower phase, coincides fall in...
Abstract Kainate‐, quisqualate‐ and glutamate‐induced depolarization mobilization of intracellular Ca 2+ was determined in primary cultured astrocytes using the fluorescent probes DiBa‐C 4 ‐(3) fura‐2, respectively. All three receptor agonists depolarized cells a Na + ‐dependent manner increased concentration. The glutamate‐ quisqualate‐induced increase cytosolic only partially inhibited by removal extracellular , whereas response to kainate totally dependent on . mechanisms for increases...
TRPA1 channels are non‐selective cation activated by plant derived pungent products including allyl isothiocyanate (AITC) from mustard. Therefore, possible intestinal secretory functions of these were investigated. We detected mRNA in mouse and human duodenal mucosa neuroendocrine STC‐1 cells. Stimulation cells with AITC increased intracellular calcium ([Ca 2+ ] i ) significantly stimulated cholecystokinin secretion 6.7‐fold. induced release was completely blocked antagonist ruthenium red...