A5036147255- Peripheral Artery Disease Management
- Diabetic Foot Ulcer Assessment and Management
- Muscle and Compartmental Disorders
- Cerebrovascular and Carotid Artery Diseases
- Muscle Physiology and Disorders
- Molecular Biology Techniques and Applications
- Carcinogens and Genotoxicity Assessment
- Diagnosis and Treatment of Venous Diseases
- Pesticide Exposure and Toxicity
- Lipoproteins and Cardiovascular Health
- Angiogenesis and VEGF in Cancer
- Cholinesterase and Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Medical and Biological Ozone Research
- Systemic Sclerosis and Related Diseases
- Immunotoxicology and immune responses
- Cardiovascular Disease and Adiposity
- Cardiovascular Health and Disease Prevention
- Connective tissue disorders research
- Musculoskeletal pain and rehabilitation
- Prostate Cancer Diagnosis and Treatment
- Toxic Organic Pollutants Impact
- Synthesis and Biological Evaluation
- Muscle metabolism and nutrition
- Electrospun Nanofibers in Biomedical Applications
University of Nebraska Medical Center
2015-2024
University of Nebraska at Omaha
2012-2024
VA Nebraska Western Iowa Health Care System
2015-2023
Casale (Switzerland)
2013-2018
Emory University
2015
Nebraska Medical Center
1989-2011
CHI Health Creighton University Medical Center - Bergan Mercy
2007
University of Florida
2007
University of Nebraska–Lincoln
1998-2007
Balseiro Institute
2007
Neuronal damage induced by ongoing human immunodeficiency virus type 1 (HIV-1) infection was investigated in humanized NOD/scid-IL-2Rγ(c)(null) mice transplanted at birth with CD34-positive hematopoietic stem cells. Mice infected 5 months of age and followed for up to 15 weeks maintained significant plasma viral loads showed reduced numbers CD4(+) T-cells. Prospective serial proton magnetic resonance spectroscopy tests selective reductions cortical N-acetyl aspartate animals. Diffusion...
Abstract Abdominal aortic aneurysm is a dynamic vascular disease characterized by inflammatory cell invasion and extracellular matrix degradation. Damage to elastin in the results release of elastin-derived peptides (EDPs), which are chemotactic for cells such as monocytes. Their effect on macrophage polarization less well known. Proinflammatory M1 macrophages initially recruited sites injury, but, if their effects prolonged, they can lead chronic inflammation that prevents normal tissue...
Peripheral arterial disease (PAD), a manifestation of systemic atherosclerosis that produces blockages in arteries supplying the legs, affects an estimated 27 million people Europe and North America. Increased production reactive oxygen species by dysfunctional mitochondria leg muscles PAD patients is viewed as key mechanism initiation progression disease. Previous studies demonstrated increased oxidative damage homogenates biopsy specimens from gastrocnemius compared to controls, but did...
A myopathy characterized by mitochondrial pathology and oxidative stress is present in patients with peripheral arterial disease (PAD). Patients PAD differ severity, mode of presentation, presence comorbid conditions. In this study, we used a mouse model hindlimb ischemia to isolate directly investigate the effects chronic inflow occlusion on skeletal muscle microanatomy, function expression, stress. Hindlimb was induced staged ligation/division common femoral iliac arteries C57BL/6 mice,...
Peripheral artery disease (PAD), a manifestation of systemic atherosclerosis that produces blockages in the arteries supplying legs, affects approximately 5% Americans. We have previously, demonstrated myopathy characterized by myofiber oxidative damage and degeneration is central to PAD pathophysiology.In this study, we hypothesized increased myofibers gastrocnemius patients myofiber-type selective correlates with reduced size.Needle biopsies were taken from 53 (28 early 25 advanced PAD)...
Patients with peripheral artery disease (PAD) develop a myopathy in their ischemic lower extremities, which is characterized by myofiber degeneration, mitochondrial dysfunction and impaired limb function. Desmin, protein of the cytoskeleton, central to maintenance structure, shape function its organelles, especially mitochondria, translation sarcomere contraction into muscle contraction. In this study, we investigated hypothesis that disruption desmin network occurs gastrocnemius myofibers...
Lower leg ischemia, myopathy, and limb dysfunction are distinguishing features of peripheral artery disease (PAD). The myopathy PAD is characterized by myofiber degeneration in association with extracellular matrix expansion, increased expression transforming growth factor-beta 1 (TGF-β1; a pro-fibrotic cytokine). In this study, we evaluated cellular TGF-β1 gastrocnemius control (CTRL) patients its relationship to deposited collagen, fibroblast accumulation hemodynamics.Gastrocnemius...
Peripheral arterial disease (PAD), which affects ∼10 million Americans, is characterized by atherosclerosis of the noncoronary arteries. PAD produces a progressive accumulation ischemic injury to legs, manifested as gradual degradation gastrocnemius histology. In this study, we evaluated hypothesis that quantitative morphological parameters myofibers change in consistent manner during progression PAD, provide an objective grading muscle degeneration limb, and correlate clinical stage PAD....
This study evaluated the hypothesis that protein concentration and mitochondrial content in gastrocnemius biopsies from patients with peripheral arterial disease (PAD) predict mortality rates.PAD experience advancing myopathy characterized by dysfunction, myofiber degradation, fibrosis their ischemic legs, along increased rates.Samples of PAD were used for all analyses. Protein was normalized to muscle wet weight, citrate synthase activity (standard measure cells) weight concentration. data...
Peripheral artery disease (PAD) pathophysiology extends beyond hemodynamics to include other operating mechanisms, including endothelial dysfunction. Oxidative stress may be linked dysfunction by reducing nitric oxide (NO) bioavailability. We aimed investigate whether the NO system and its regulators are altered in setting of PAD assess relationship between bioavailability oxidative stress. Sera from 35 patients with intermittent claudication (IC), 26 critical limb ischemia (CLI), non-PAD...
Thoracic aortic aneurysm and dissection are life-threatening complications of Marfan syndrome (MFS). Studies human mouse samples from late stage MFS demonstrate increased TGF-β activation/signaling diffuse matrix changes. However, the role smooth muscle cell (SMC) phenotype in early formation has yet to be fully elucidated. As our objective, we investigated whether an altered SMC plays a MFS. We describe previously unrecognized concordant findings aortas murine model MFS, mgR, during...
Work from our laboratory documents pathological events, including myofiber oxidative damage and degeneration, myofibrosis, micro-vessel (diameter = 50–150 μm) remodeling, collagenous investment of terminal micro-vessels ≤ 15 µm) in the calf muscle patients with Peripheral Artery Disease (PAD). In this study, we evaluate hypothesis that vascular pathology associated legs PAD encompasses pathologic changes to smallest muscle. Biopsies were collected control subjects Fontaine Stage II IV PAD....
Abstract Objective Neuroinflammation associated with anti‐N‐methyl‐D‐aspartate receptor (NMDAR) encephalitis may facilitate seizures. We previously showed that intraventricular administration of cerebrospinal fluid from patients anti‐NMDAR to mice precipitates seizures, thereby confirming antibodies are directly pathogenic. To determine whether interleukin (IL)‐1‐mediated inflammation exacerbates autoimmune we asked blocking the effects IL‐1 by anakinra, a selective antagonist, blunts...
Polycyclic aromatic hydrocarbons (PAH) are metabolized to electrophiles that can bind DNA bases and destabilize the N-glycosyl bond, causing rapid depurination of adducted bases. Recent studies support as a mechanism central genesis H-ras mutations in PAH-treated mouse skin. Depurinating adducts account for 71% all formed skin treated with benzo[a]pyrene (BP). This study analyzed urine cigarette smokers, coal smoke-exposed women, nonexposed controls presence quantities depurinated...
The anticholinesterase (antiCHE) insecticides, a large family of pesticides used extensively throughout the world, inhibit serine hydrolases by carbamylating or phosphorylating residue at catalytic site. These insecticides are viewed as potential inhibitors hydrolase-dependent immune functions including interleukin 2 (IL2) signalling. Previous studies in our laboratory have demonstrated that carbaryl (an antiCHE insecticide) produces marked concentration-dependent inhibition IL2 driven 1)...
Peripheral artery disease (PAD) is a common atherosclerotic characterized by narrowed or blocked arteries in the lower extremities. Circulating serum biomarkers can provide significant insight regarding progression. Here, we explore metabolomics signatures associated with different stages of PAD and investigate potential mechanisms disease. We compared metabolites cohort 26 patients presenting claudication critical limb ischemia (CLI) to those non-PAD controls. A difference between...
Abstract Uridine diphosphate ( UDP)‐glucose dehydrogenase (UGDH) catalyzes the oxidation of UDP‐glucose to yield UDP‐glucuronic acid, a precursor for synthesis glycosaminoglycans and proteoglycans that promote aggressive prostate cancer (PC) progression. The purpose our study was determine if UGDH expression in normal appearing acini (NAA) from cancerous glands is candidate biomarker PC field disease/effect assayed by quantitative fluorescence imaging analysis (QFIA). A polyclonal antibody...