A5000654347- Acute Kidney Injury Research
- Chronic Kidney Disease and Diabetes
- Renal and Vascular Pathologies
- Cardiac Ischemia and Reperfusion
- Renal and related cancers
- Angiogenesis and VEGF in Cancer
- Muscle and Compartmental Disorders
- Electrolyte and hormonal disorders
- Semiconductor materials and devices
- Sodium Intake and Health
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Climate Change and Health Impacts
- Nitric Oxide and Endothelin Effects
- Dialysis and Renal Disease Management
- Semiconductor Quantum Structures and Devices
- Semiconductor materials and interfaces
- Organ Donation and Transplantation
- Birth, Development, and Health
- Renal function and acid-base balance
- Renal cell carcinoma treatment
- Thermoregulation and physiological responses
- Renin-Angiotensin System Studies
- Organ Transplantation Techniques and Outcomes
- Kidney Stones and Urolithiasis Treatments
- Mesenchymal stem cell research
Indiana University – Purdue University Indianapolis
2016-2025
Indiana University School of Medicine
2014-2024
Indiana University Health
2016-2024
IRCCS Policlinico San Donato
2018-2023
Indiana University Indianapolis
2022
Cancer Genetics (United States)
2017-2021
Suzuki (Japan)
2020-2021
Center for Translational Molecular Medicine
2017-2021
Health & Life (Taiwan)
2017-2021
Délégation Paris 6
2020-2021
Acute episodes of severe renal ischemia result in acute failure (ARF). These are followed by a characteristic recovery and repair response, whereby tubular morphology function appear completely restored within approximately 1 mo. However, the chronic effects such an injury have not been well studied. Male rats were subjected to 60-min bilateral reperfusion, yielding injury. Postischemic animals manifested diuresis, peaking at wk postinjury (volume: >45 ml/day, ARF vs. 18 sham; P < 0.05)....
The purpose of this study was to assess the effectiveness a traditional lecture-based curriculum versus modified "flipped classroom" cardiovascular, respiratory, and renal physiology delivered first-year graduate students. Students in both courses were provided same notes recorded lectures. flipped classroom required watch prerecorded lectures before class then attend class, where they received quiz or homework covering material each lecture (valued at 25% final grade) followed by question...
Recent clinical studies indicate a strong link between AKI and progression of CKD. The increasing prevalence must compel the nephrology community to consider long-term ramifications this syndrome. Considerable gaps in knowledge exist regarding connection 13th Acute Dialysis Quality Initiative meeting entitled "Therapeutic Targets Human Kidney Injury: Harmonizing Experimental Animal Injury" convened April 2014 assigned working group focus on issues related after AKI. This article provides...
Acute kidney injury induces the loss of renal microvessels, but fate endothelial cells and mechanism potential vascular growth factor (VEGF)-mediated protection is unknown. Cumulative cell proliferation was analyzed in Sprague-Dawley rats following ischemia-reperfusion (I/R) by repetitive administration BrdU (twice daily) colocalization with CD31 or cablin. Proliferating were undetectable for up to 2 days I/R accounted only ∼1% BrdU-positive after 7 days. VEGF-121 preserved did not affect...
First published August 9, 2001; 10.1152/ajprenal.00050.2001.—Acute episodes of severe renal ischemia result in acute failure (ARF). These are followed by a characteristic recovery and repair response, whereby tubular morphology function appear completely restored within ∼1 mo. However, the chronic effects such an injury have not been well studied. Male rats were subjected to 60-min bilateral reperfusion, yielding injury. Postischemic animals manifested diuresis, peaking at 1 wk postinjury...
Si nanowires grow rapidly by chemical vapor deposition on Ti-containing islands surfaces when an abundant supply of Si-containing gaseous precursor is available. The density wires approximately the same as nucleating surface, although at least two different types appear to correlate with very wire growth rates. For conditions used, a minority long, defect-free form, along more numerous containing defects. Energy-dispersive x-ray spectroscopy shows that nanoparticles remain tip growing wires....
Reductions in vascular density occur following acute ischemia-reperfusion (I/R) injury that may predispose the development of chronic kidney disease. The mechanisms mediating loss are not clear but relate to lack effective repair responses. To determine regulation VEGF/VEGFR pathway I/R injury, male Sprague-Dawley rats were subjected bilateral renal ischemia (45 min) and allowed recover for 1, 3, 7, 35 days. VEGF mRNA expression was repressed by greater than 50% control values up 3 days...
Acute kidney injury induced by renal ischemia-reperfusion (I/R) compromises microvascular density and predisposes to chronic disease (CKD) sodium-dependent hypertension. VEGF-121 was administered rats fed a standard (0.4%) sodium diet at various times following recovery from I/R for up 35 days. had no effect on the initial loss of function, as indicated serum creatinine levels measured 24 h after injury. Serum declined thereafter, indicative repair. Rats were then switched an elevated (4.0%)...
Most circulating endothelial cells are apoptotic, but rare colony-forming (C-ECFCs), also known as blood outgrowth cells, with proliferative and vasculogenic activity can be cultured; however, the origin naive function of these C-ECFCs remains obscure. Herein, detailed lineage tracing revealed murine emerged in early postnatal period, displayed high potential enriched frequency clonal compared tissue-resident ECFCs, were not committed to or derived from BM hematopoietic system ECFCs. In...
Ischemic acute renal failure (ARF) results in the permanent loss of peritubular capillaries and predisposes progression chronic failure. The present study was undertaken to determine whether hypoxia, which may represent an important mediator disease progression, is persistently exacerbated after recovery from ARF. Rats were subjected ischemia-reperfusion injury allowed recover for 5 or 20 wk. Immunohistochemistry hypoxia-sensitive marker 2-pimonidizole at wk revealed overall increase...
Ischemic injury to the kidney results in blood vessel loss and predisposition chronic renal disease. Angiostatin is a proteolytic cleavage product of plasminogen that inhibits angiogenesis, promotes apoptosis endothelial cells, disrupts capillary integrity. A combination lysine-Sepharose enrichment followed by Western blotting was used study expression angiostatin response induction ischemic injury. No products were readily detectable kidneys sham-operated control rats. In contrast, both 38-...
Recovery of renal function is a well-characterized feature models acute failure; however, more recent studies have reported predisposition to chronic disease. This study sought determine the susceptibility sodium-dependent hypertension following recovery from ischemic failure. Following ischemia-reperfusion (I/R) injury, rats were allowed recover for 35 days on 0.4% salt diet, then switched 4.0% diet an additional 28 days. Blood pressure was significantly increased in postischemic...
T cells have been implicated in the pathogenesis of acute kidney injury (AKI) and its progression to chronic disease (CKD). Previous studies suggest that Th17 participate during AKI-to-CKD transition, inhibition cell activity by mycophenolate mofetil (MMF) or losartan attenuates development fibrosis following AKI. We hypothesized cell-deficient rats may reduced levels IL-17 cytokine leading decreased Renal ischemis-reperfusion (I/R) was performed on athymic (Foxn1rnu-/rnu-) control euthymic...
ANG II is a potent renal vasoconstrictor and profibrotic factor its activity enhanced by oxidative stress. We sought to determine whether stress was persistent following recovery from acute kidney injury (AKI) induced ischemia-reperfusion (I/R) in rats this resulted increased sensitivity. Rats were allowed recover bilateral I/R for 5 wk blood flow responses measured. Post-AKI showed significantly relative sham-operated controls treatment of AKI with apocynin (15 mM, the drinking water)...
To gain insight into the role that transforming growth factor-beta 1 (TGF-beta 1) plays in regeneration of kidneys following acute renal failure, we characterized expression TGF-beta mRNA and active latent peptide at various times during recovery from ischemic injury rat. Levels whole kidney were elevated significantly 12 h postinjury (1.5-fold vs. sham-operated controls), by 24 3.6-fold. remained for 14 days ischemia, but no longer 28 postinjury. In situ hybridization demonstrated was...
To define potential roles for bcl-2 and bax in adult kidney as regulators of regeneration, their expressions were characterized postischemic injury. A 2.1-fold increase levels renal mRNA occurred within 24 h injury relative to sham-operated control rats. The remained elevated 3 days but returned baseline by day 5 postischemia. In situ hybridization kidneys from rats demonstrated faint expression localized diffusely throughout the nephron. After injury, was markedly enhanced regenerating...