A5054124420- Corneal surgery and disorders
- Glaucoma and retinal disorders
- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Ocular Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Intraocular Surgery and Lenses
- Ophthalmology and Visual Impairment Studies
- Nitric Oxide and Endothelin Effects
- Glycosylation and Glycoproteins Research
- Olfactory and Sensory Function Studies
- Neuropeptides and Animal Physiology
- Zebrafish Biomedical Research Applications
- CRISPR and Genetic Engineering
- Fungal Biology and Applications
- Sulfur Compounds in Biology
- Eicosanoids and Hypertension Pharmacology
- Ocular Diseases and Behçet’s Syndrome
- Pluripotent Stem Cells Research
- Microbial Natural Products and Biosynthesis
- Peptidase Inhibition and Analysis
- Neuroscience and Neuropharmacology Research
- Biochemical Analysis and Sensing Techniques
- Angiogenesis and VEGF in Cancer
- Plant Micronutrient Interactions and Effects
International University of Health and Welfare
2021-2025
Mita Hospital
2021-2024
Nara Medical University
2017-2023
Osaka University
2011-2023
University of Tokyo Hospital
2018-2023
Nara Medical University Hospital
2022
The University of Tokyo
1995-2021
Mie University
2015-2021
Kanazawa Medical University
1996-2019
Johns Hopkins Medicine
2011-2015
Most circulating endothelial cells are apoptotic, but rare colony-forming (C-ECFCs), also known as blood outgrowth cells, with proliferative and vasculogenic activity can be cultured; however, the origin naive function of these C-ECFCs remains obscure. Herein, detailed lineage tracing revealed murine emerged in early postnatal period, displayed high potential enriched frequency clonal compared tissue-resident ECFCs, were not committed to or derived from BM hematopoietic system ECFCs. In...
Abstract DNA transposons and retroviruses are versatile tools in functional genomics gene therapy. To facilitate their application, we conducted a genome-wide insertion site profiling of the piggyBac (PB), Tol2 Sleeping Beauty (SB) murine leukemia virus (MLV) mouse embryonic stem cells (ESCs). PB MLV preferred highly expressed genes, whereas SB weakly genes. However, correlations with DNase I hypersensitive sites were different for all vectors, indicating that chromatin accessibility is not...
To develop a collagen vitrigel (CV) optimized as corneal endothelial cell (CEC) carrier and create an artificial graft.We first developed flat-shaped for regenerative medicine (CV-RM) using porcine atelocollagen ultraviolet (UV) irradiation. The optimal UV amount was determined by measuring the CV-RM transparency under various irradiating conditions. treatment (CV-CERT), transparent with curved shape, made spherically molds membrane permeability of CV-CERT tested in vitro. biocompatibility,...
Excessive accumulation of nickel (Ni) can be toxic to plants. In Arabidopsis thaliana, the Fe2+ transporter, iron (Fe)-regulated transporter1 (IRT1), mediates Fe uptake and also implicates in Ni2+ at roots; however, underlying mechanism remains unelucidated. present study, we found that zinc (Zn) deficient conditions resulted increased Ni plants, particularly roots, A. thaliana. order elucidate mechanisms correlating condition, traced 63Ni isotope response Zn (i) deficiency induces...
<title>Abstract</title> This study investigated the corneal epithelial thickness and its distribution in keratoconus (KC) forme fruste (FFK). We recruited 144 KC eyes 27 FFK from 76 participants (50 men 26 women) who underwent anterior segment-optical coherence tomography April 2020 to September 2023 at International University of Health Welfare Narita Hospital. There were 42 age- sex-matched controls. Corneal central, paracentral, peripheral areas statistically investigated. Each area was...
Staphylococcus epidermidis is one of the prominent pathogens in ocular infection. The prevalence mutations quinolone resistance determining region (QRDR) area S isolated from surface and its association with fluoroquinolone has not been fully elucidated.Mutations QRDR gyrA, gyrB, parC, parE genes 138 isolates recovered human conjunctival flora were analysed. minimal inhibitory concentrations (MICs) four fluoroquinolones (levofloxacin, gatifloxacin, moxifloxacin tosufloxacin) against these...
Factor V (FV) plays pivotal roles in both procoagulant and anticoagulant mechanisms. Genetic mutations, FV-W1920R (FVNara) FV-A2086D (FVBesançon), the C1 C2 domains of FV light chain, respectively, seem to be associated with deep vein thrombosis. However, detailed mechanism(s) through which these mutations are linked thrombophilia remains fully explored. The aim this study was clarify thrombotic presence abnormalities. Full-length wild-type (WT) mutated were prepared using stable, human cell...
Purpose: To evaluate the effect of transplanting bioengineered corneal endothelial grafts in a rabbit model failure.Methods: Human cells (HCECs) were seeded on vitrigel carrier. After Descemet's membrane was removed from eyes rabbits, transplantation done with vitrigel/HCEC graft or alone without cells, left untreated. Slit lamp examinations and measurement central thickness (CCT) performed for 14 days postoperatively.Results: HCECs cultured strongly positive ZO-1 Na+/K+ ATPase. On day 14,...
To determine the aqueous humor concentrations of moxifloxacin and besifloxacin after routine preoperative topical dosing in patients having cataract surgery.Wilmer Eye Institute, Johns Hopkins University, Baltimore, Maryland, USA.In this prospective randomized parallel double-masked clinical trial, 1 drop commercially available 0.5% ophthalmic solution or 0.6% suspension was administered every 10 minutes for a total 4 doses beginning hour before surgery. Aqueous sampled via paracentesis,...
Three Kampo medicines, Boiogito (BOT), Bofutsushosan (BTS) and Orengedokuto (OGT), used for obese patients were investigated their effects on adipogenesis in cultured rat white adipocytes. Administration of the three extracts suppressed concentration-dependent manners (1—100 μg/ml) without any cytotoxicity. Changes mRNA expression levels analyzed using a Rat 230 2.0 Affymetrix GeneChip® microarray system. DNA analysis (total probe set: 31099) cDNAs prepared from adipocytes revealed that BOT,...
A new species of the genus Dactylosporangium is described, for which name fulvum proposed. In addition to finger-shaped sporangia, this organism produced abundant globose bodies and coremia. It differs significantly from previously described in its cultural physiological characteristics. The type strain D. SF-2113 (IFO 14381).
We previously reported emergence and disappearance of circadian molecular oscillations during differentiation mouse embryonic stem (ES) cells reprogramming differentiated cells, respectively. Here we present a robust stringent in vitro clock formation assay that recapitulates vivo phenotypes. This system first confirmed mutant ES cell line lacking Casein Kinase I delta (CKIδ) induced ∼3 hours longer period-length rhythm than the wild type, which was compatible with recently results using...
<h3>Aim</h3> To evaluate the stromal bed quality and endothelial damage after femtosecond laser (FSL) cuts into deep corneal stroma. <h3>Methods</h3> Using a 150-kHz FSL, lamellar cut was aimed at depth of 100, 300, or 500 μm in porcine corneas. Stromal smoothness graded from light microscopy scanning electron images. Rabbit corneas were remaining thicknesses 70, 100 150 using FSL. The effects peeling off flap distance between spots (2 4 μm) examined. <h3>Results</h3> ratio damaged cells...
Abstract Background: A school urinary screening (SUS) system has been conducted for 30 years in Japan, but the cross‐sectional data have never reported or analyzed. The purpose of present study was to analyze epidemiologically. Methods: All elementary and junior high children public Tokyo who had SUS performed by Health Service Association from 1974 2002 (approx. 400 000–600 000 per year) were involved. analyzed with Pearson’s correlation coefficient. Results: During first 10 SUS, prevalence...
Purpose: To compare the clinical characteristics of acute hydrops between eyes with recurrent keratoconus and treatment-naïve keratoconus.Methods: Six caused by (recurrent group) 29 due to (naïve were included. We collected data from anterior-segment optical coherence tomography (AS-OCT), corneal topography medical records. With AS-OCT, we investigated location Descemet's membrane break, length frequency detachment, speed thinning before hydrops.Results: breaks detectable in 6 cases group...
A b initio molecular orbital–configuration-interaction (CI) calculations at full valence CI level as well the SDTQ-CI on and Rydberg MOs were carried out potential energy hypersurfaces in lower singlet states along one probable reaction path of decomposition from SiH4 to 2SiH+2H+1H2. The result shows that there exists a one-step route which forms radiative species SiH*(A 2Δ) spontaneously. adiabatic following explain straightforwardly various observations plasma- photo-CVD experiments SiH4....
Gamma irradiated corneas in which the donor keratocytes and endothelial cells are eliminated effective as corneal lamellar glaucoma patch grafts. In addition, gamma irradiation causes collagen cross inking, stiffens fibrils. This study evaluated for use transplantations a rabbit model comparing graft clarity, neovascularization, edema.Penetrating keratoplasty was performed on rabbits using four types of grafts: Fresh cornea with endothelium, cornea, cryopreserved fresh without endothelium....