A5083724046- Hemophilia Treatment and Research
- Blood Coagulation and Thrombosis Mechanisms
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Hemostasis and retained surgical items
- Chronic Myeloid Leukemia Treatments
- Blood groups and transfusion
- Blood properties and coagulation
- Heparin-Induced Thrombocytopenia and Thrombosis
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Venous Thromboembolism Diagnosis and Management
- Antiplatelet Therapy and Cardiovascular Diseases
- Atrial Fibrillation Management and Outcomes
- Protease and Inhibitor Mechanisms
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Renal Diseases and Glomerulopathies
- Complement system in diseases
- Virus-based gene therapy research
- Infrared Thermography in Medicine
- Mechanical Circulatory Support Devices
- Muscle and Compartmental Disorders
- Coronary Interventions and Diagnostics
- Nasolacrimal Duct Obstruction Treatments
- Blood transfusion and management
- Aortic aneurysm repair treatments
Nara Medical University
2015-2024
Nara Medical University Hospital
2015-2024
Pediatrics and Genetics
2022
Queen's University
2014-2020
May Institute
2010
Fundación Juan March
2010
Introduction A recently developed method to assess comprehensive coagulation function, clot waveform analysis ( CWA ), accurately detect low levels (<1 IU/dL) of factor VIII activity (FVIII:C) in haemophilia patients (HA‐pts). Improvements are needed, however, differentiate with very from absent FVIII :C. Aim We attempted optimize using the analyser CS ‐2000i™ distinguish between and :C severe HA ‐pts. Methods Results Activated partial thrombin time aPTT )‐based waveforms were determined...
Factor VIIIa functions as a cofactor for factor IXa in the phospholipid surface-dependent activation of X. Both C2 domain and Gla are involved binding required In this study, we have examined close relationship between these domains Xase complex. Enzyme-linked immunosorbent assay-based surface plasmon resonance-based assays absence showed that Glu-Gly-Arg active site-modified bound to immobilized recombinant (rC2) dose-dependently (Kd = 108 nm). This ability was optimal under physiological...
Summary Acquired haemophilia A (AHA) is caused by the development of factor (F)VIII autoantibodies, demonstrating type 1 or 2 inhibitory behaviour, and results in more serious haemorrhagic symptoms than congenital severe HA. The reason(s) for this remains unknown, however. global coagulation assays, thrombin generation tests clot waveform analysis, demonstrated that parameters patients with AHA-type inhibitor were significantly depressed those moderate HA similar FVIII activities. Thrombin...
Summary Background: Systemic coagulation disorders after cardiac surgery represent serious postoperative complications. There have been few reports, however, identifying preoperative tests that predict bleeding. The aim of the present study was to investigate relationship between hemorrhage and parameters determined by global assays, define potential predictive markers. Methods: Twenty‐one pediatric patients were enrolled. Blood samples collected before 24 h surgery. Laboratory...
Simultaneous evaluation of coagulation and fibrinolysis facilitates an overall understanding normal pathological haemostasis. We established assay for assessing clot formation simultaneously using waveform analysis by the trigger a mixture activated partial thromboplastin time reagent optimized concentration tissue-type plasminogen activator (0·63 μg/ml) to examine temporal reactions in short monitoring (<500 s). The interplay between was confirmed analysing effects argatroban, tranexamic...
Factor V (FV) plays pivotal roles in both procoagulant and anticoagulant mechanisms. Genetic mutations, FV-W1920R (FVNara) FV-A2086D (FVBesançon), the C1 C2 domains of FV light chain, respectively, seem to be associated with deep vein thrombosis. However, detailed mechanism(s) through which these mutations are linked thrombophilia remains fully explored. The aim this study was clarify thrombotic presence abnormalities. Full-length wild-type (WT) mutated were prepared using stable, human cell...
Summary The diagnosis of von Willebrand disease ( VWD ) is difficult due to the wide spectrum clinical phenotypes associated with this disorder. We have analysed and characterized haemostatic function in patients using a microchip‐based flow chamber system. Microchips coated either collagen [platelet PL )‐chip] or collagen/thromboplastin [atherome AR were used evaluate platelet thrombus formation at 1000 s −1 fibrin‐rich 240 respectively. Blood samples from an asymptomatic patient type 1...
Plasminogen activators provide effective treatment for patients with acute myocardial infarction. However, paradoxical elevation of thrombin activity associated failure clot lysis and recurrent thrombosis has been reported. Generation in these circumstances appears to be owing plasmin (Plm)-induced activation factor (F) XII. Plm catalyzes proteolysis several coagulant factors, but the roles factors on Plm-mediated procoagulant remain determined. Recently developed global coagulation assays...