A5071084218- Microbial Natural Products and Biosynthesis
- Bioactive Compounds and Antitumor Agents
- Hemophilia Treatment and Research
- Blood Coagulation and Thrombosis Mechanisms
- Synthesis and biological activity
- Marine Sponges and Natural Products
- Fungal Biology and Applications
- Cardiac electrophysiology and arrhythmias
- Phenothiazines and Benzothiazines Synthesis and Activities
- Natural product bioactivities and synthesis
- Pharmacogenetics and Drug Metabolism
- Mycobacterium research and diagnosis
- Synthesis of β-Lactam Compounds
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Synthesis and Biological Activity
- NF-κB Signaling Pathways
- Game Theory and Voting Systems
- Phytochemistry and Bioactive Compounds
- Algal biology and biofuel production
- Chronic Myeloid Leukemia Treatments
- Drug Transport and Resistance Mechanisms
- Carbohydrate Chemistry and Synthesis
- Platelet Disorders and Treatments
- Solar and Space Plasma Dynamics
- Cancer therapeutics and mechanisms
Nara Medical University
2023-2025
Nara Medical University Hospital
2024
Keio University
2000-2023
Showa University
2018
Clinical Research Institute
2018
Nutrasource
2006
Microbial Chemistry Research Foundation
1996-2002
Keio Shiki Senior High School
2002
Central Research Laboratories (United Kingdom)
2001
We previously designed and synthesized an NF-κB inhibitor, dehydroxymethylepoxyquinomicin (DHMEQ), that showed anti-inflammatory activity <i>in vivo</i>. In the present study we looked into its mechanism of inhibition. DHMEQ inhibited tumor necrosis factor-α (TNF-α)- 12-<i>O</i>-tetradecanoylphorbol-13-acetate-induced transcriptional in human T cell leukemia Jurkat cells. It also TNF-α-induced DNA binding nuclear but not phosphorylation degradation IκB. Moreover, accumulation p65, a...
Two hundred thirteen cytochrome P450 (P450) genes were collected from bacteria and expressed based on an Escherichia coli expression system to test their hydroxylation ability testosterone. Twenty-four P450s stereoselectively monohydroxylated testosterone at the 2α-, 2β-, 6β-, 7β-, 11β-, 12β-, 15β-, 16α-, 17-positions (17-hydroxylation yields 17-ketoproduct). The site usage of is not same as that human P450s, while 17-hydroxylation are reactions common both bacterial P450s. Most catalyzed by β face.
BackgroundEmicizumab is a humanized bispecific monoclonal antibody that bridges activated factor IX (FIXa) and X (FX) to mimic the function of VIII (FVIII). It suppresses bleeding tendency in hemophilia A patients with or without FVIII inhibitors. case an adult inhibitor‐positive patient whom treatment emicizumab was discontinued owing repeated events prolonged partial thromboplastin time.ObjectiveTo analyze mechanisms decreased efficacy emicizumab.MethodsResidual plasma samples were used...
While the conventional understanding of haemostatic plug formation is that coagulation proceeds efficiently on surface activated platelets at vascular injury site to form a robust plug, this does not explain clinical reality platelet dysfunction results in mild bleeding phenotype, whereas disorders exhibit severe phenotypes, particularly deep tissues. Here, we introduce an vivo imaging method observe internal and subsequent mice report after bleeding, occurs primarily outside blood vessel...
A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only small amount of was produced, probably due poor permeability into E. coli cell. In an alternative approach cell-free reaction system, conversion ratio increased up 17%.
Purpose In relation to institution type and employment status, this study aims identify the requisite skills, knowledge attitudes (SKAs) listed in job advertisements for Japanese library staff. Design/methodology/approach This collected data on 545 posts advertised major librarian advertisement websites from March 2019 January 2021. The included type, length, application requirements descriptions. Job descriptions were classified into 16 categories based required SKAs. Data analyzed using...
Personalized PageRank (PPR) is a popular graph computation in various real-world applications. Since massive graphs are evolving rapidly, PPR methods require index-free and fast. In general, go through Forward Push phase random walk Monte-Carlo simulation respectively. While existing have succeeded accelerating the phase, there space for running-time improvements second that performs large number of sequential walks. Through this process, each needs to obtain neighbor nodes every single...
Abstract Background In patients with hemophilia (PwH), bleeding often occurs in joints and muscles, early detection of hemorrhage is important to prevent the onset progression mobility impairment. Complex-Image analysis such as ultrasonography, computed tomography, magnetic resonance imaging are used detect bleeding. On other hand, no simple rapid method active has been reported. Local inflammatory responses occur when blood leaks from damaged vessels, temperature at site could be expected...
Two types of thermophilic, spore-forming, strict anaerobes were isolated from spoiled canned “Shiruko” (a kind soft drink made red beans and cane sugar) coffee containing milk which had been produced for retailing through an automatic hot vending machine in the winter season. The products typically showed no swelling can, but offensive odor slightly reduced pH value content, as a result production acetic acid.The organisms identified Desulfotomaculum nigrificans (group A) Clostridium...
Abstract Background Joint damage in patients with haemophilia (PwH) is commonly assessed by imaging, but few reports have described how structural changes joints, for example, haemophilic arthropathy (HA)—affect gait ability. Objectives We evaluated function among PwH HA, without and people (non‐PwH) using a Zebris FDM‐T treadmill (FDM‐T), an easy‐to‐use assessment instrument force sensor matrix. Methods The following parameters were collected: centre of pressure trajectory intersection...