A5046121155- Immunotherapy and Immune Responses
- Lymphatic System and Diseases
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Nanoparticle-Based Drug Delivery
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- RNA Interference and Gene Delivery
- Platelet Disorders and Treatments
- Nanoplatforms for cancer theranostics
- Cancer, Stress, Anesthesia, and Immune Response
- Proteoglycans and glycosaminoglycans research
- Neuropeptides and Animal Physiology
- Immune Cell Function and Interaction
- Blood properties and coagulation
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Drug Delivery Systems
- Microfluidic and Bio-sensing Technologies
- Fluid Dynamics and Turbulent Flows
- Inhalation and Respiratory Drug Delivery
- Heat Transfer and Boiling Studies
- Neonatal Respiratory Health Research
- Planarian Biology and Electrostimulation
Georgia Institute of Technology
2016-2025
The Wallace H. Coulter Department of Biomedical Engineering
2016-2025
Emory University
2015-2024
Indian Institute of Technology Madras
2024
Central Council for Research in Ayurvedic Science
2023
Parker Hannifin (United States)
2020-2022
Cancer Institute (WIA)
2015-2022
Yonsei University
2022
Winship Cancer Institute
2017-2019
University of Utah
2006-2017
Tumor expression of the lymphangiogenic factor VEGF-C is correlated with metastasis and poor prognosis, although enhances transport to draining lymph node (dLN) antigen exposure adaptive immune system, its role in tumor immunity remains unexplored. Here, we demonstrate that promotes tolerance murine melanoma. In B16 F10 melanomas expressing a foreign (OVA), protected tumors against preexisting antitumor promoted local deletion OVA-specific CD8+ T cells. Naive cells, transferred into...
Accumulating evidence implicates the tumor-draining lymph node (TDLN) in tumor-induced immune escape, as it drains regulatory molecules and leukocytes from tumor microenvironment. We asked whether targeted delivery of adjuvant to TDLN, presumably already bathed antigens, could promote anti-tumor immunity hinder growth. To this end, we used 30 nm polymeric nanoparticles (NPs) that effectively target dendritic cells (DCs, CD11c(+)) within (LN) after intradermal administration. These NPs...
Anticancer therapeutic effects can be improved by directing immune checkpoint blockade antibodies to tumor-draining lymph nodes.
Abstract Due to their autosynchronous roles in shaping the anti-tumor immune response, complex regulatory networks acting both locally within tumor microenvironment as well its draining lymph nodes play critical cancer immunotherapy response. We describe herein a thermosensitive co-polymer hydrogel system formed from biocompatible polymers gelatin and Pluronic ® F127 that are widely used humans enable sustained release of nitric oxide donor antibody blocking checkpoint cytotoxic...
Selectin-mediated adhesion of tumor cells to platelets, leukocytes, and endothelial may regulate their hematogenous dissemination in the microvasculature. We recently identified CD44 variant isoforms (CD44v) as functional P-, but not E- or L-, selectin ligands on colon carcinoma cells. Moreover, an approximately 180-kDa sialofucosylated glycoprotein(s) mediated binding CD44-knockdown Using immunoaffinity chromatography tandem mass spectrometry, we identify this glycoprotein carcinoembryonic...
Abstract Lymphatic vessels transport interstitial fluid, soluble Ag, and immune cells from peripheral tissues to lymph nodes (LNs), yet the contribution of lymphatic drainage adaptive immunity remains poorly understood. We examined responses dermal vaccination contact hypersensitivity (CHS) challenge in K14-VEGFR-3-Ig mice, which lack capillaries experience markedly depressed solutes dendritic skin draining LNs. In response immunization, mice produced lower Ab titers. contrast, although...
Therapeutic immunomodulation in the skin, its draining lymph nodes, or both tissues simultaneously using an intradermal administration scheme is desirable for a variety of therapeutic scenarios. To inform how drug carriers comprising engineered biomaterials can be leveraged to improve treatment efficacy by enhancing selective accumulation retention payload within these target tissues, we analyzed influence particle versus macromolecule hydrodynamic size on profiles site dermal injection as...
Hydrogels present versatile platforms for the encapsulation and delivery of proteins cells regenerative medicine applications. However, differences in hydrogel cross-linking density, polymer weight content, affinity all contribute to diverse diffusion rates through networks. Here, we describe a simple method accurately measure protein hydrogels, within few hours without use large amounts protein. We tracked several varying molecular weights along axial direction capillary tubes filled with...
Selectins and fibrin(ogen) play key roles in the hematogenous dissemination of tumor cells, especially colon carcinomas. However, receptor(s) on carcinoma cells has yet to be defined along with its relative capacity bind fibrinogen versus fibrin under flow. Moreover, functional P-selectin ligand validated using intact platelets rather than purified selectin substrates. Using human CD44-knockdown control LS174T we demonstrate pivotal involvement CD44 P-selectin-mediated binding shear...
Tissue remodeling is a characteristic of many solid tumor malignancies including melanoma. By virtue lymphatic transport, pathways active within the local microenvironment have potential to be operational lymph nodes (LNs) draining interstitium. Here, we show that drainage from murine B16 melanomas in syngeneic, immune-competent C57Bl/6 mice associated with LN enlargement as well nonuniform increases bulk tissue elasticity and viscoelasticity, measured by response whole LNs compression....
Abstract Molecular events activating the PI3K pathway are frequently detected in human tumors and activation of signaling alters numerous cellular processes including tumor cell proliferation, survival, motility. More recent studies have highlighted impact on response to interferons other immunologic relevant antitumor immunity. Given ability IFNγ regulate antigen processing presentation pivotal role MHC class I (MHCI) II (MHCII) expression T-cell–mediated immunity, we sought determine MHCI...
A lymph node sinus-on-a-chip adhesion microfluidic platform that recapitulates the hydrodynamic microenvironment of subcapsular sinus was engineered. This device used to interrogate effects remodeling on cellular in fluid flow relevant lymphatic metastasis. Wall shear stress levels analytically estimated and modeled after quiescent diseased/inflamed nodes were experimentally recapitulated using a flow-based perfusion system assess physiological fields human metastatic cancer cell adhesion....
After separating from a primary tumor, metastasizing cells enter the circulatory system and interact with host before lodging in secondary organs. Previous studies have implicated surface glycoproteins CD44 carcinoembryonic antigen (CEA) adhesion, migration, invasion, suggesting that they may influence metastatic progression. To elucidate role of these multifunctional molecules while avoiding potential drawbacks ectopic expression or monoclonal antibody treatments, we silenced and/or CEA...