A5021018333- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- SARS-CoV-2 and COVID-19 Research
- Nanofabrication and Lithography Techniques
- Advanced biosensing and bioanalysis techniques
- Mesenchymal stem cell research
- Gold and Silver Nanoparticles Synthesis and Applications
- Graphene and Nanomaterials Applications
- Lymphatic System and Diseases
- Erythrocyte Function and Pathophysiology
- Iron oxide chemistry and applications
- CAR-T cell therapy research
- Tissue Engineering and Regenerative Medicine
- Advanced Biosensing Techniques and Applications
- Respiratory viral infections research
- Lipid Membrane Structure and Behavior
- Characterization and Applications of Magnetic Nanoparticles
- Electrospun Nanofibers in Biomedical Applications
- vaccines and immunoinformatics approaches
- Advanced Nanomaterials in Catalysis
- Bone Tissue Engineering Materials
- Complement system in diseases
- Nanocluster Synthesis and Applications
University of Chicago
2021
École Polytechnique Fédérale de Lausanne
2014-2020
Bioengineering (Switzerland)
2017
Vanderbilt University
2010-2013
Center for Nanoscale Science and Technology
2012-2013
Vanderbilt University Medical Center
2011
Rice University
2010-2011
Phospholipid bilayers that constitute endo-lysosomal vesicles can pose a barrier to delivery of biologic drugs intracellular targets. To overcome this barrier, number synthetic drug carriers have been engineered actively disrupt the endosomal membrane and deliver cargo into cytoplasm. Here, we describe hemolysis assay, which be used as rapid, high-throughput screen for cytocompatibility endosomolytic activity systems. In human red blood cells test materials are co-incubated in buffers at...
Abstract: The assessment of macrophage response to nanoparticles is a central component in the evaluation new nanoparticle designs for future vivo application. This work investigates which feature, size or charge, more predictive non-specific uptake by macrophages. was investigated synthesizing library polymer-coated iron oxide micelles, spanning range 30–100 nm diameter and -23 mV +9 mV, measuring internalization into macrophages vitro. Nanoparticle charge both contributed towards uptake,...
Macrophages represent an important therapeutic target, because their activity has been implicated in the progression of debilitating diseases such as cancer and atherosclerosis. In this work, we designed characterized pH-responsive polymeric micelles that were mannosylated using "click" chemistry to achieve CD206 (mannose receptor)-targeted siRNA delivery. is primarily expressed on macrophages dendritic cells upregulated tumor-associated macrophages, a potentially useful target for therapy....
Abstract Small interfering RNA (siRNA) has significant potential to evolve into a new class of pharmaceutical inhibitors, but technologies that enable robust, tissue‐specific intracellular delivery must be developed before effective clinical translation can achieved. A pH‐responsive, smart polymeric nanoparticle (SPN) with matrix metalloproteinase (MMP)‐7‐dependent proximity‐activated targeting (PAT) is described here. The PAT‐SPN designed trigger cellular uptake and cytosolic siRNA once...
Chronic inflammation-mediated oxidative stress is a common mechanism of implant rejection and failure. Therefore, polymer scaffolds that can degrade slowly in response to this environment may provide viable platform for site-specific, sustained release immunomodulatory agents over long time period. In work, proline oligomers varying lengths (P(n)) were synthesized exposed environments, their accelerated degradation under conditions was verified via high performance liquid chromatography gel...
Lymphatic endothelial cells (LECs) chemoattract naïve T and promote their survival in the lymph nodes, can cross-present antigens to CD8+ drive proliferation despite lacking key costimulatory molecules. However, functional consequence of LEC priming is unknown. Here, we show that while many proliferating LEC-educated enter early apoptosis, remainders comprise a long-lived memory subset, with transcriptional, metabolic, phenotypic features central stem cell-like cells. In vivo, these...
The COVID-19 pandemic underscores the need for rapid, safe, and effective vaccines. In contrast to some traditional vaccines, nanoparticle-based subunit vaccines are particularly efficient in trafficking antigens lymph nodes, where they induce potent immune cell activation. Here, we developed a strategy decorate surface of oxidation-sensitive polymersomes with multiple copies SARS-CoV-2 spike protein receptor-binding domain (RBD) mimic physical form virus particle. We evaluated vaccination...
Myocardial infarction results in extensive cardiomyocyte death which can lead to fatal arrhythmias or congestive heart failure. Delivery of stem cells repopulate damaged cardiac tissue may be an attractive and innovative solution for repairing the heart. Instructive polymer scaffolds with a wide range properties have been used extensively direct differentiation cells. In this study, we optimized chemical mechanical electrospun mesh directed embryonic (ESCs) towards cardiomyogenic lineage. A...
The SARS-CoV-2 virus has caused an unprecedented global crisis, and curtailing its spread requires effective vaccine which elicits a diverse robust immune response. We have previously shown that vaccines made of polymeric glyco-adjuvant conjugated to antigen were in triggering such response other disease models hypothesized the technology could be adapted create against SARS-CoV-2. core platform is copolymer p(Man-TLR7), composed monomers with pendant mannose or toll-like receptor 7 (TLR7)...
Abstract The implementation of engineered surfaces presenting micrometer‐sized patterns cell adhesive ligands against a biologically inert background has led to numerous discoveries in fundamental biology. While existing surface patterning strategies allow single ligand, it is still challenging fabricate displaying multiple patterned ligands. To address this issue we implemented laser scanning lithography (LSL), laser‐based thermal desorption technique, multifaceted, micropatterned that...
Inflammation and angiogenesis are inevitable in vivo responses to biomaterial implants. Continuous progress has been made design improve tissue interactions with an implant by either reducing inflammation or promoting angiogenesis. However, it become increasingly clear that the physiological processes of interconnected through various molecular mechanisms. Hence, there is unmet need for engineering functional tissues simultaneous activation pro-angiogenic anti-inflammatory In this work,...
Abstract Background Drug and contrast agent delivery systems that achieve controlled release in the presence of enzymatic activity are becoming increasingly important, as is a hallmark wide array diseases, including cancer atherosclerosis. Here, we have synthesized clusters ultrasmall superparamagnetic iron oxides (USPIOs) sense for applications magnetic resonance imaging (MRI). To this goal, utilize amphiphilic poly(propylene sulfide)- bl -poly(ethylene glycol) (PPS-b-PEG) copolymers, which...
Phospholipid bilayers that constitute endo-lysosomal vesicles can pose a barrier to delivery of biologic drugs intracellular targets. To overcome this barrier, number synthetic drug carriers have been engineered actively disrupt the endosomal membrane and deliver cargo into cytoplasm. Here, we describe hemolysis assay, which be used as rapid, high-throughput screen for cytocompatibility endosomolytic activity systems. In human red blood cells test materials are co-incubated in buffers at...
The assessment of macrophage response to nanoparticles is a central component in the evaluation new nanoparticle designs for future vivo application.This work investigates which feature, size or charge, more predictive non-specific uptake by macrophages.This was investigated synthesizing library polymer-coated iron oxide micelles, spanning range 30-100 nm diameter and -23 mV +9 mV, measuring internalization into macrophages vitro.Nanoparticle charge both contributed towards uptake, but...
ABSTRACT A diverse portfolio of SARS-CoV-2 vaccine candidates is needed to combat the evolving COVID-19 pandemic. Here, we developed a subunit nanovaccine by conjugating Spike protein receptor binding domain (RBD) surface oxidation-sensitive polymersomes. We evaluated humoral and cellular responses mice immunized with these surface-decorated polymersomes (RBD surf ) compared RBD-encapsulated encap unformulated RBD free ), using monophosphoryl lipid A-encapsulated (MPLA PS) as an adjuvant....
Myocardial infarction results in extensive cardiomyocyte death which can lead to fatal arrhythmias or congestive heart failure.Delivery of stem cells repopulate damaged cardiac tissue may be an attractive and innovative solution for repairing the heart.Instructive polymer scaffolds with a wide range properties have been used extensively direct differentiation cells.In this study, we optimized chemical mechanical electrospun mesh directed embryonic (ESCs) towards cardiomyogenic lineage.A...
The frontispiece features the multistrata nanoparticle—a single-core, five-layered nanostructure. It combines tunable, dual-peak, UV–vis–NIR spectrum extinction characteristics; trimodal imaging contrast; a simple synthesis; and facile surface modification capabilities into single <60-nm-diameter, multifunctional nanosphere that seeks to relieve current methodological limitations by coupling diagnostics therapeutics one theranostic tool. image shows model of interior, multilayered,...
The implementation of engineered surfaces with micrometer-sized patterns cell adhesive ligands against a biologically inert background has led to numerous discoveries in fundamental biology. On page 2876, Jennifer L. West and co-workers present laser-based patterning strategy for the fabrication displaying multiple patterned ligands, where each ligand is confined its own array. Cells cultured on two display concurrent adhesion both simultaneously utilize lamella formation during migration.
Abstract Tumor associated macrophages (TAMs) can modify the tumor microenvironment to create an inflammatory, pro-tumor niche. Activation of NF-κB pathway has been implicated in creating a phenotype TAMs. Manipulation TAM is new approach engage anticancer immunity, but limited by lack methods capable therapeutic delivery TAMs vivo. We have successfully utilized mannosylated polymer nanoparticles disrupting endosomal compartment deliver siRNA for RNAi proteins into bone marrow derived (BMDMs)...