A5031750482- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Crop Yield and Soil Fertility
- Nanoparticle-Based Drug Delivery
- Muscle Physiology and Disorders
- Electron and X-Ray Spectroscopy Techniques
- Electrospun Nanofibers in Biomedical Applications
- Semiconductor materials and devices
- Advancements in Photolithography Techniques
- Gas Dynamics and Kinetic Theory
- Agricultural Science and Fertilization
- Soil Carbon and Nitrogen Dynamics
- RNA and protein synthesis mechanisms
- Angiogenesis and VEGF in Cancer
- Integrated Circuits and Semiconductor Failure Analysis
- Metal and Thin Film Mechanics
- Dendrimers and Hyperbranched Polymers
- Hydraulic Fracturing and Reservoir Analysis
- Insect Resistance and Genetics
- Industrial Vision Systems and Defect Detection
- Cancer, Hypoxia, and Metabolism
- Aluminum Alloys Composites Properties
- Peripheral Artery Disease Management
University of Arkansas at Fayetteville
2020-2025
University of North Carolina at Chapel Hill
2024
North Carolina State University
2024
Duke University
2014-2023
Dow Chemical (United States)
1951-2023
Rutherford Appleton Laboratory
2020
Duke Medical Center
2015-2017
Vanderbilt University
2011-2016
Intel (United States)
2016
Nashville Oncology Associates
2014-2015
Editing can help build stronger muscles Much of the controversy surrounding gene-editing technology called CRISPR/Cas9 centers on ethics germline editing human embryos to correct disease-causing mutations. For certain disorders such as muscular dystrophy, it may be possible achieve therapeutic benefit by faulty gene in somatic cells. In proof-of-concept studies, Long et al. , Nelson and Tabebordbar used adeno-associated virus-9 deliver system young mice with a mutation coding for dystrophin,...
Phospholipid bilayers that constitute endo-lysosomal vesicles can pose a barrier to delivery of biologic drugs intracellular targets. To overcome this barrier, number synthetic drug carriers have been engineered actively disrupt the endosomal membrane and deliver cargo into cytoplasm. Here, we describe hemolysis assay, which be used as rapid, high-throughput screen for cytocompatibility endosomolytic activity systems. In human red blood cells test materials are co-incubated in buffers at...
A family of pH-responsive diblock polymers composed poly[(ethylene glycol)-b-[(2-(dimethylamino)ethyl methacrylate)-co-(butyl methacrylate)], PEG-(DMAEMA-co-BMA), was reversible addition-fragmentation chain transfer (RAFT) synthesized with 0-75 mol % BMA in the second polymer block. The relative mole DMAEMA and varied order to identify a that can be used formulate PEGylated, siRNA-loaded polyplex nanoparticles (NPs) an optimized balance cationic hydrophobic content NP core based on siRNA...
Adeno-associated virus (AAV)-mediated CRISPR-Cas9 editing holds promise to treat many diseases. The immune response bacterial-derived Cas9 has been speculated as a hurdle for AAV-CRISPR therapy. However, immunological consequences of AAV-mediated expression have thus far not thoroughly investigated in large mammals. We evaluate Cas9-specific responses canine models Duchenne muscular dystrophy (DMD) following intramuscular and intravenous Treatment results initially robust dystrophin...
CRISPR-Cas9 transcriptional repressors have emerged as robust tools for disrupting gene regulation in vitro but not yet been adapted systemic delivery adult animal models. Here we describe a Staphylococcus aureus Cas9-based repressor (dSaCas9
Macrophages represent an important therapeutic target, because their activity has been implicated in the progression of debilitating diseases such as cancer and atherosclerosis. In this work, we designed characterized pH-responsive polymeric micelles that were mannosylated using "click" chemistry to achieve CD206 (mannose receptor)-targeted siRNA delivery. is primarily expressed on macrophages dendritic cells upregulated tumor-associated macrophages, a potentially useful target for therapy....
A system is engineered for temporally controlled delivery of siRNA from biodegradable tissue regenerative scaffolds. Therapeutic application this approach to silence PHD2 promotes expression pro-angiogenic genes by HIF1α and enhanced scaffold vascularization in vivo. This technology provides a new standard efficient controllable gene silencing modulate host response within biomaterials. As service our authors readers, journal supporting information supplied the authors. Such materials are...
Abstract Small interfering RNA (siRNA) has significant potential to evolve into a new class of pharmaceutical inhibitors, but technologies that enable robust, tissue‐specific intracellular delivery must be developed before effective clinical translation can achieved. A pH‐responsive, smart polymeric nanoparticle (SPN) with matrix metalloproteinase (MMP)‐7‐dependent proximity‐activated targeting (PAT) is described here. The PAT‐SPN designed trigger cellular uptake and cytosolic siRNA once...
Adeno-associated virus–mediated (AAV-mediated) CRISPR editing is a revolutionary approach for treating inherited diseases. Sustained, often life-long mutation correction required these Unfortunately, this has never been demonstrated with AAV therapy. We addressed question in the mdx model of Duchenne muscular dystrophy (DMD). DMD caused by dystrophin gene mutation. Dystrophin deficiency leads to ambulation loss and cardiomyopathy. treated 6-week-old mice intravenously evaluated disease...
The adsorption of H2O on a single-crystal α-Al2O3(0001) surface was examined using laser-induced thermal desorption (LITD) and temperature-programmed (TPD) techniques. models the Al2O3 exhaust particles generated by solid rocket motors that may affect stratospheric ozone layer. After cleaning annealing to 1100 K, displayed well-defined hexagonal (1 × 1) low-energy electron diffraction (LEED) pattern. Absolute hydroxyl coverages this were determined LITD signals. Hydroxylation dissociative...
Small interfering RNA (siRNA) delivered from reactive oxygen species-degradable tissue engineering scaffolds promotes diabetic wound healing in rats. Porous poly(thioketal-urethane) implanted wounds locally deliver siRNA that inhibits the expression of prolyl hydroxylase domain protein 2, thereby increasing progrowth genes and vasculature, proliferating cells, development wounds.
Genetically engineered mouse models that employ site-specific recombinase technology are important tools for cancer research but can be costly and time-consuming. The CRISPR-Cas9 system has been adapted to generate autochthonous tumours in mice, how these compare generated by conventional remains fully explored. Here we use multiple subtypes of primary sarcomas efficiently wild type genetically mice. These data demonstrate used soft tissue Primary with Cre had similar histology, growth...
A dual-targeted siRNA nanocarrier has been synthesized and validated that is selectively activated in environments where there colocalization of two breast cancer hallmarks, elevated matrix metalloproteinase (MMP) activity folate receptor overexpression. This self-assembled from polymers containing the same pH-responsive, endosomolytic core-forming block but varying hydrophilic, corona-forming blocks. The corona one polymer consists a 2 kDa PEG attached to terminal folic acid (FA); second...