A5100676757- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Electrospun Nanofibers in Biomedical Applications
- Nanoparticle-Based Drug Delivery
- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Wound Healing and Treatments
- Cell Adhesion Molecules Research
- Developmental Biology and Gene Regulation
- Nanoplatforms for cancer theranostics
- Protein Tyrosine Phosphatases
- Pressure Ulcer Prevention and Management
- Angiogenesis and VEGF in Cancer
- Mesenchymal stem cell research
- Cancer-related Molecular Pathways
- Congenital heart defects research
- Diabetic Foot Ulcer Assessment and Management
- MicroRNA in disease regulation
- Microtubule and mitosis dynamics
- Total Knee Arthroplasty Outcomes
- Coronary Interventions and Diagnostics
- Dialysis and Renal Disease Management
- Infectious Aortic and Vascular Conditions
- Planarian Biology and Electrostimulation
- Inflammasome and immune disorders
Peking University
2018-2025
Vanderbilt University
2015-2025
Covance (United States)
2023
Daping Hospital
2023
Army Medical University
2023
Cornell University
2023
Ministry of Education of the People's Republic of China
2018-2021
Vanderbilt University Medical Center
2014-2016
Beijing Shijitan Hospital
2015
Capital Medical University
2015
Porous, resorbable biomaterials can serve as temporary scaffolds that support cell infiltration, tissue formation, and remodeling of nonhealing skin wounds. Synthetic are less expensive to manufacture than biologic dressings achieve a broader range physiochemical properties, but opportunities remain tailor these materials for ideal host immune regenerative responses. Polyesters well-established class synthetic biomaterials; however, acidic degradation products released by their hydrolysis...
In addition to increasing insulin sensitivity and adipogenesis, peroxisome proliferator-activated receptor (PPAR)-gamma agonists cause weight gain hyperphagia. Given the central role of brain in control energy homeostasis, we sought determine whether PPARgamma is expressed key areas involved metabolic regulation. Using immunohistochemistry, distribution its colocalization with neuron-specific protein markers were investigated rat mouse sections spanning hypothalamus, ventral tegmental area,...
A system is engineered for temporally controlled delivery of siRNA from biodegradable tissue regenerative scaffolds. Therapeutic application this approach to silence PHD2 promotes expression pro-angiogenic genes by HIF1α and enhanced scaffold vascularization in vivo. This technology provides a new standard efficient controllable gene silencing modulate host response within biomaterials. As service our authors readers, journal supporting information supplied the authors. Such materials are...
Although siRNA-based nanomedicines hold promise for cancer treatment, conventional siRNA–polymer complex (polyplex) nanocarrier systems have poor pharmacokinetics following intravenous delivery, hindering tumor accumulation. Here, we determined the impact of surface chemistry on in vivo and delivery siRNA polyplexes. A library diblock polymers was synthesized, all containing same pH-responsive, endosomolytic polyplex core-forming block but different corona blocks: 5 kDa (benchmark) 20 linear...
Background: How the extracellular matrix (ECM) microenvironment modulates contractile phenotype of vascular smooth muscle cells (VSMCs) and confers homeostasis remains elusive. Methods: To explore key ECM proteins in maintenance VSMCs, we applied protein-protein interaction network analysis to novel associated with VSMC phenotype. By combining vitro vivo genetic mice injury models, identified nidogen-2, a basement membrane glycoprotein, as protein for cell identity. Results: We collected...
Small interfering RNA (siRNA) delivered from reactive oxygen species-degradable tissue engineering scaffolds promotes diabetic wound healing in rats. Porous poly(thioketal-urethane) implanted wounds locally deliver siRNA that inhibits the expression of prolyl hydroxylase domain protein 2, thereby increasing progrowth genes and vasculature, proliferating cells, development wounds.
Minimal delivery of E-selectin–TRAIL liposomes can effectively target postsurgical metastasis in triple-negative breast cancer.
Post-traumatic osteoarthritis (PTOA) associated with joint injury triggers a degenerative cycle of matrix destruction and inflammatory signaling, leading to pain loss function. Here, prolonged RNA interference (RNAi) metalloproteinase 13 (MMP13) is tested as PTOA disease modifying therapy. MMP13 upregulated in degrades the key cartilage structural protein type II collagen. Short interfering (siRNA) loaded nanoparticles (siNPs) were encapsulated shape-defined poly(lactic-
Cisplatin is one of the most effective chemotherapeutics, but its usefulness limited by toxicity to normal tissues, including cells kidney proximal tubule. The purpose these studies was determine mechanism cisplatin cytotoxicity. It shown in vivo that administration induces upregulation gene for p21 cyclin-dependent kinase (cdk) inhibitor cells. This protein a positive effector on fate cisplatin-exposed renal tubule and vitro; adenoviral transduction completely protected from toxicity....
The complexity of CRISPR machinery is a challenge to its application for nonviral in vivo therapeutic gene editing. Here, we demonstrate that proteins, regardless size or charge, efficiently load into porous silicon nanoparticles (PSiNPs). Optimizing the loading strategy yields formulations are ultrahigh loading─>40% cargo by volume─and highly active. Further tuning polymeric coating on loaded PSiNPs nanocomposites achieve colloidal stability under cryopreservation, endosome escape, and...
As a hallmark of chronic kidney disease (CKD), arterial stiffening is related to increased vascular inflammation and cardiovascular morbidity, whereas the underlying mechanism unclear. The study demonstrates that stiffness precedes onset inflammation, matrix stimulates transdifferentiation smooth muscle cells (VSMCs) an inflammatory phenotype via activating Runx2-NLRP3 signaling, which provides novel insights into CKD-related disorder treatment.
Abstract Alterations in the phosphatidylinositol 3-kinase (PI3K) signaling / mammalian target of rapamycin (mTOR) pathway are often seen triple negative breast cancers (TNBCs), a cancer subtype characterized by limited molecularly targeted treatment options and poorer patient outcomes. We report that gene amplification or overexpression mTORC2-required cofactor RICTOR correlated with increased mTORC2 worse outcome clinical BC expression datasets, supporting studies examining selective...
<p>RICTOR-amplified and RICTOR-diploid TNBC cell lines</p>
<p>Combined mTORC1/2 inhibition is more effective than selective mTORC1 in TNBC. <b>A,</b> The distinct effects of TNBC were studied using RAD001, whereas combined dual mTOR kinase inhibitor PP242. Western blot analysis whole-cell lysates harvested 24 hours after treatment with DMSO, RAD001 (500 nmol/L), or PP242 nmol/L) 10% serum. Antibodies used are indicated to the left each panel. <b>B</b> and <b>C,</b> Cell titer (<b>B</b>)...
<p>Validation of target silencing activity siRictor and siRaptor</p>
<p>Primer sequences</p>
<p>siRictor-NPs on target silencing and induction of apoptosis in TNBCs</p>
<p>Development and cell growth testing of siMTOR</p>
<p>PI3K-mTOR signaling pathway</p>