A5001867561- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Silicon Nanostructures and Photoluminescence
- MicroRNA in disease regulation
- Immune Response and Inflammation
- Bone Tissue Engineering Materials
- Neuroscience and Neural Engineering
- Lipid Membrane Structure and Behavior
- Bacterial biofilms and quorum sensing
- Radioactive element chemistry and processing
- Nanowire Synthesis and Applications
- Microbial Metabolism and Applications
- Pickering emulsions and particle stabilization
- Antimicrobial Peptides and Activities
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- interferon and immune responses
- Graphene and Nanomaterials Applications
- Analytical chemistry methods development
Vanderbilt University
2019-2024
University of South Carolina
2014-2017
Abstract Peptides and biologics provide unique opportunities to modulate intracellular targets not druggable by conventional small molecules. Most peptides are fused with cationic uptake moieties or formulated into nanoparticles facilitate delivery, but these systems typically lack potency due low and/or entrapment degradation in endolysosomal compartments. Because most delivery reagents comprise lipids polymers, there is a of specifically optimized deliver cargo. Herein, we demonstrate the...
The complexity of CRISPR machinery is a challenge to its application for nonviral in vivo therapeutic gene editing. Here, we demonstrate that proteins, regardless size or charge, efficiently load into porous silicon nanoparticles (PSiNPs). Optimizing the loading strategy yields formulations are ultrahigh loading─>40% cargo by volume─and highly active. Further tuning polymeric coating on loaded PSiNPs nanocomposites achieve colloidal stability under cryopreservation, endosome escape, and...
RNA ligands of retinoic acid-inducible gene I (RIG-I) hold significant promise as antiviral agents, vaccine adjuvants, and cancer immunotherapeutics, but their efficacy is hindered by inefficient intracellular delivery to the cytosol where RIG-I localized. Here, we address this challenge through synthesis evaluation a library polymeric carriers rationally designed promote endosomal escape 5′-triphosphate (3pRNA) agonists. We synthesized series PEG-block-(DMAEMA-co-AnMA) polymers, AnMA an...
Abstract Objectives Zein nanoparticles (Zein NPs) were used as a hydroxyapatite (HA) biomineralization template to generate HA/Zein NPs. Doxorubicin hydrochloride (DOX) was loaded on NPs (HA/Zein-DOX improve its pH-sensitive release, bioavailability and decrease cardiotoxicity. Methods HA/Zein-DOX prepared by phase separation biomimetic mineralization method. Particle size, polydispersity index (PDI), Zeta potential, transmission electron microscope, X-ray diffraction Fourier-transform...
Porous silicon nanoparticles (PSNPs) offer tunable pore structure and easily modified surface chemistry, enabling high loading capacity for drugs with diverse chemicophysical properties. While PSNPs are also cytocompatible degradable, PSNP integration into composite structures can be a useful approach to enhance carrier colloidal stability, drug-cargo endosome escape. Here, we explored polymer composites formed by coating of oxidized series poly[ethylene glycol-block-(dimethylaminoethyl...
The separation of hazardous metals from contaminated sources is commonly achieved with ion-exchange resins. resins have a high surface area decorated many sites and thus sorption capacity for the analyte interest. However, these are primarily accessed by diffusion which limits throughput quality separation. Reported herein study monolithic polyHIPE foam columns surface-grafted brush polymer containing functionality Pu. It was found that loading curves material steeper than similarly scaled...
Rapid, facile, and noncovalent cell membrane modification with alkyl-grafted anionic polymers was sought as an approach to enhance intracellular delivery bioactivity of cationic peptides. We synthesized a library acrylic acid-based copolymers containing varying amounts amine-reactive pentafluorophenyl acrylate monomer followed by postpolymerization series alkyl amines afford precise control over the length density aliphatic side chains. This synthetic strategy enabled systematic...
siRNA therapeutics have considerable potential as molecularly-targeted in malignant disease, but identification of effective delivery strategies that mediate rapid intracellular while minimizing toxicity has been challenging. Our group recently developed and optimized an conjugate platform termed "siRNA-L