A5026339730- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Circular RNAs in diseases
- Bioinformatics and Genomic Networks
- Computational Drug Discovery Methods
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Aortic aneurysm repair treatments
- Machine Learning in Bioinformatics
- Metabolomics and Mass Spectrometry Studies
- Atherosclerosis and Cardiovascular Diseases
- Pancreatic and Hepatic Oncology Research
- Ferroptosis and cancer prognosis
- Ubiquitin and proteasome pathways
- Gastric Cancer Management and Outcomes
- Alzheimer's disease research and treatments
- Liver Disease Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Neuroscience and Neuropharmacology Research
Peking University
2016-2025
Zhejiang University
2018-2025
Huizhou Central People's Hospital
2025
Hangzhou Normal University
2023-2024
Shanghai Maritime University
2022-2024
Sir Run Run Shaw Hospital
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2024
Peking University Third Hospital
2024
Southeast University
2024
Nantong University
2024
Comprehensive databases of microRNA-disease associations are continuously demanded in biomedical researches. The recently launched version 3.0 Human MicroRNA Disease Database (HMDD v3.0) manually collects a significant number miRNA-disease association entries from literature. Comparing to HMDD v2.0, this new contains 2-fold more entries. Besides, the have been accurately classified based on literature-derived evidence code, which results six generalized categories (genetics, epigenetics,...
N6-methyladenosine (m6A) is a prevalent RNA methylation modification involved in the regulation of degradation, subcellular localization, splicing and local conformation changes transcripts. High-throughput experiments have demonstrated that only small fraction m6A consensus motifs mammalian transcriptomes are modified. Therefore, accurate identification sites becomes emergently important. For above purpose, here computational predictor site named SRAMP established. To depict sequence...
MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression and play vital roles in various biological processes. It has been reported that aberrant regulation miRNAs was associated with the development progression diseases, but underlying mechanisms not fully deciphered. Here, we described our updated TransmiR v2.0 database for more comprehensive information about transcription factor (TF)-miRNA regulations. 3730 TF-miRNA regulations among 19 species from 1349...
Compelling evidence indicates that epigenetic regulations orchestrate dynamic macrophage polarization. N6-methyladenosine (m6A) methylation is the most abundant modification of mammalian mRNA, but its role in polarization still completely unknown. Here, we show m6A-catalytic enzyme methyltransferase like 3 (METTL3) specifically upregulated following M1 mouse macrophages. Furthermore, METTL3 knockdown through siRNA transfection markedly inhibited M1, enhanced M2, Conversely, overexpression...
With the rapid accumulation of high-throughput microRNA (miRNA) expression profile, up-to-date resource for analyzing functional and disease associations miRNAs is increasingly demanded. We here describe updated server TAM 2.0 miRNA set enrichment analysis. Through manual curation over 9000 papers, a more than two-fold growth reference sets has been achieved in comparison with previous TAM, which covers 9945 1584 newly collected miRNA-disease miRNA-function associations, respectively....
Abstract Sex differences are widely observed under various circumstances ranging from physiological processes to therapeutic responses, and a myriad of sex-biased genes have been identified. In recent years, transcriptomic datasets microRNAs (miRNAs), an important class non-coding RNAs, become increasingly accessible. However, comprehensive analysis sex difference in miRNA expression has not performed. Here, we identified the differentially-expressed miRNAs between males females by examining...
The mitophagy receptor FUNDC1 interacts with FBXL2 to preserve mitochondrial Ca 2+ homeostasis and cardiac function in obesity.
Background: How the extracellular matrix (ECM) microenvironment modulates contractile phenotype of vascular smooth muscle cells (VSMCs) and confers homeostasis remains elusive. Methods: To explore key ECM proteins in maintenance VSMCs, we applied protein-protein interaction network analysis to novel associated with VSMC phenotype. By combining vitro vivo genetic mice injury models, identified nidogen-2, a basement membrane glycoprotein, as protein for cell identity. Results: We collected...
Phenotypic transition of vascular smooth muscle cells (VSMCs) accounts for the pathogenesis a variety diseases during early stage. Recent studies indicate metabolic reprogramming may be involved in VSMC phenotypic transition. However, definite molecules that link energy metabolism to distinct phenotype remain elusive.A carotid artery injury model was used study postinjury neointima formation as well vivo. RNA-seq analysis, cell migration assay, collagen gel contraction wire myography...
Abstract Distinct from the traditional diagnostic/prognostic biomarker (adopted as indicator of disease state/process), therapeutic (ThMAR) has emerged to be very crucial in clinical development and practice all therapies. There are five types ThMAR that have been found play indispensable roles various stages drug discovery, such as: Pharmacodynamic Biomarker essential for guaranteeing pharmacological effects a therapy, Safety critical assessing extent or likelihood therapy-induced toxicity,...
Abstract NAT10-catalyzed N4-acetylcytidine (ac4C) has emerged as a vital post-transcriptional modulator on the coding transcriptome by promoting mRNA stability. However, its role in mammalian development remains unclear. Here, we found that NAT10 expression positively correlates with pluripotency vivo and vitro. High throughput ac4C-targeted RNA immunoprecipitation sequencing (ac4C-RIP-seq), NaCNBH3-based chemical ac4C (ac4C-seq) liquid chromatography-tandem mass spectrometry (LC–MS/MS)...
Abstract Background The cytotoxicity of NK cells is largely dependent on IgG Fc receptor CD16a, which mediates antibody-dependent cell-mediated (ADCC). high-affinity and non-cleavable CD16 (hnCD16) developed demonstrated a multi-tumor killing potential. However, the hnCD16 activates single signal provides limited tumor suppression. How to exploit properties incorporate cell-specific activation domains promising development direction further improve anti-tumor activity cells. Methods To...
Doxorubicin (DOX) is among the most widely employed antitumor agents, although its clinical applications have been largely hindered by severe cardiotoxicity. Earlier studies described an essential role of mitochondrial injury in pathogenesis DOX cardiomyopathy. PHB2 (Prohibitin 2) perceived as regulator for dynamics and oxidative phosphorylation (OXPHOS) involvement cardiomyopathy remains elusive.To decipher possible cardiomyopathy, tamoxifen-induced cardiac-specific conditional knockout...
Abstract While deep learning (DL)-based models have emerged as powerful approaches to predict protein–protein interactions (PPIs), the reliance on explicit similarity measures (e.g. sequence and network neighborhood) known interacting proteins makes these methods ineffective in dealing with novel proteins. The advent of AlphaFold2 presents a significant opportunity also challenge PPIs straightforward way based monomer structures while controlling bias from protein sequences. In this work, we...
The ECM (extracellular matrix) is a major component of the vascular microenvironment that modulates homeostasis. proteins include collagens, elastin, noncollagen glycoproteins, and proteoglycans/glycosaminoglycans. form complex matrix structures, such as basal lamina collagen elastin fibers, through direct interactions or lysyl oxidase-mediated cross-linking. Moreover, directly interact with cell surface receptors extracellular secreted molecules, exerting matricellular matricrine...
Background: Vascular smooth muscle cell (VSMC) migration and proliferation substantially contribute to neointimal hyperplasia related in-stent restenosis. N6-methyladenosine (m6A) catalyzed by the methyltransferase-like 3 (METTL3)-involved methyltransferase complex is most abundant RNA epigenetic modification in eukaryotes, but role of m6A methylation VSMC as well neointima formation remains highly controversial. Methods: Primary human rat VSMCs were utilized for vitro experiments....
Alzheimer disease (AD) is characterized by neurodegeneration marked loss of synapses and spines associated with hyperphosphorylation tau protein. Accumulating amyloid β peptide (Aβ) in brain linked to neurofibrillary tangles composed hyperphosphorylated AD. Here, we identify β2-adrenergic receptor (β2AR) that mediates Aβ-induced pathology. In the prefrontal cortex (PFC) 1-year-old transgenic mice human familial mutant genes presenilin 1 precursor protein (PS1/APP), phosphorylation at Ser-214...
// Chun-Ming Wong 1 , Lai Wei Sandy Leung-Kuen Au Dorothy Ngo-Yin Fan Yuan Zhou 2 Felice Ho-Ching Tsang Cheuk-Ting Law Joyce Man-Fong Lee Xianghuo He 3 Jue Shi Carmen Chak-Lui and Irene Oi-Lin Ng Department of Pathology State Key Laboratory for Liver Research, Li Ka Shing Faculty Medicine, The University Hong Kong, China Physics Biology, Centre Quantitative Systems Kong Baptist University, Oncogenes Related Genes, Shanghai Cancer Institute, Jiao Tong School Shanghai, Correspondence to: Wong,...
Prokaryotic proteins are regulated by pupylation, a type of post-translational modification that contributes to cellular function in bacterial organisms. In pupylation process, the prokaryotic ubiquitin-like protein (Pup) tagging is functionally analogous ubiquitination order tag target for proteasomal degradation. To date, several experimental methods have been developed identify pupylated and their sites, but these generally laborious costly. Therefore, computational can accurately predict...