A5007226303- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- COVID-19 epidemiological studies
- Biosensors and Analytical Detection
- Viral Infections and Outbreaks Research
- Respiratory viral infections research
- COVID-19 and Mental Health
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Advanced biosensing and bioanalysis techniques
- HIV Research and Treatment
- Disaster Response and Management
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- RNA Research and Splicing
- Telemedicine and Telehealth Implementation
- Data-Driven Disease Surveillance
- HIV/AIDS drug development and treatment
- Influenza Virus Research Studies
- Nuclear Structure and Function
- COVID-19 diagnosis using AI
- COVID-19 Digital Contact Tracing
- Virology and Viral Diseases
- Mathematical and Theoretical Epidemiology and Ecology Models
Heidelberg University
2014-2023
University Hospital Heidelberg
2014-2020
National Institute of Allergy and Infectious Diseases
2016
National Institutes of Health
2014-2016
Abstract Amyloid fibrils result from the aggregation of host cell-encoded proteins, many giving rise to specific human illnesses such as Alzheimer’s disease. Here we show that major virulence factor Rift Valley fever virus, protein NSs, forms filamentous structures in brain mice and affects mortality. NSs assembles into nuclear cytosolic disulfide bond-dependent fibrillary aggregates infected cells. structural arrangements exhibit characteristics typical for amyloids, an ultrastructure 12...
Middle East respiratory syndrome coronavirus (MERS-CoV) is a recently isolated betacoronavirus identified as the etiologic agent of frequently fatal disease in Western Asia, syndrome. Attempts to identify natural reservoirs MERS-CoV have focused part on dromedaries. Bats are also suspected be based frequent detection other betacoronaviruses these mammals. For this study, ten distinct cell lines derived from bats divergent species were exposed MERS-CoV. Plaque assays, immunofluorescence and...
Little is known about the repertoire of cellular factors involved in replication pathogenic alphaviruses. To uncover molecular regulators alphavirus infection, and to identify candidate drug targets, we performed a high-content imaging-based siRNA screen. We revealed an actin-remodeling pathway involving Rac1, PIP5K1- α, Arp3, as essential for infection by Infection causes actin rearrangements into large bundles filaments termed foci. Actin foci are generated late concomitantly with envelope...
Abstract Throughout the SARS‐CoV‐2 pandemic, limited diagnostic capacities prevented sentinel testing, demonstrating need for novel testing infrastructures. Here, we describe setup of a cost‐effective platform that can be employed in high‐throughput manner, which allows surveillance as an acute pandemic control and preparedness tool, exemplified by diagnostics academic environment. The strategy involves self‐sampling based on gargling saline, pseudonymized sample handling, automated RNA...
ABSTRACT Human immunodeficiency virus type 1 (HIV-1) infection of dividing and nondividing cells involves regulatory interactions with the nuclear pore complex (NPC), followed by translocation to nucleus preferential integration into genomic areas in proximity inner membrane (INM). To identify host proteins that may contribute these processes, we performed an overexpression screen known membrane-associated NE proteins. We found integral transmembrane SUN1/UNC84A SUN2/UNC84B are potent or...
The COVID-19 pandemic has entered its third year and continues to affect most countries worldwide. Active surveillance, i.e. testing individuals irrespective of symptoms, presents a promising strategy accurately measure the prevalence SARS-CoV-2. We aimed identify cost-effective active surveillance for among four strategies tested in randomised control trial between 18th November 2020 23rd December Germany. included: (A1) direct individuals; (A2) households; (B1) conditioned on upstream...
Background Despite the important role of testing as a measure against COVID-19 pandemic, user perspectives on SARS-CoV-2 tests remain scarce, inhibiting an improvement approaches. As world enters third year more nuanced testing, and opportunities to expand in feasible affordable manner merit consideration. Methods Conducted amid second pandemic wave (late 2020–early 2021) during after multi-arm trial evaluating surveillance strategies federal state Baden-Württemberg, Germany, this...
To achieve higher effectiveness in population-based SARS-CoV-2 surveillance and to reliably predict the course of an outbreak, screening, monitoring infected individuals without major symptoms (about 40% population) will be necessary. While current testing capacities are also used identify such asymptomatic cases, this rather passive approach is not suitable generating reliable estimates prevalence carriers allow any dependable predictions on pandemic.This trial implements a two-factorial,...
HIV-1 formation is driven by the viral structural polyprotein Gag, which assembles at plasma membrane into a hexagonal lattice. The C-terminal p6Gag domain harbors short peptide motifs, called late domains, recruit cellular endosomal sorting complex required for transport and promote abscission from membrane. Similar to containing proteins of other viruses, p6 phosphorylated multiple residues, including highly conserved serine position 40. Previously published studies showed that an S40F...
Background The COVID-19 pandemic is characterized by rapid increases in infection burden owing to the emergence of new variants with higher transmissibility and immune escape. To date, monitoring has mainly relied on passive surveillance, yielding biased epidemiological measures disproportionate number undetected asymptomatic cases. Active surveillance could provide accurate estimates true prevalence forecast evolution pandemic, enabling evidence-based decision-making. Objective This study...
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Abstract Throughout the current SARS-CoV-2 pandemic, limited diagnostic testing capacity prevented sentinel of population, demonstrating need for novel strategies and infrastructures. Here, we describe set-up an alternative platform, which allows scalable surveillance as acute pandemic response tool preparedness purposes, exemplified by diagnostics in academic environment. The strategy involves self-sampling based on gargling saline, pseudonymized sample handling, automated 96-well...
<sec> <title>BACKGROUND</title> The COVID-19 pandemic is characterized by rapid increases in infection burden owing to the emergence of new variants with higher transmissibility and immune escape. To date, monitoring has mainly relied on passive surveillance, yielding biased epidemiological measures disproportionate number undetected asymptomatic cases. Active surveillance could provide accurate estimates true prevalence forecast evolution pandemic, enabling evidence-based decision-making....