Abstract Objective Collagen membranes serve as barriers to separate bone grafts from soft tissues. Bone harvested with a scraper release growth factors activating transforming factor‐β ( TGF ‐β) signaling in mesenchymal cells. The aim of the present pilot study was determine whether collagen adsorb molecules bone‐conditioned medium BCM ) capacity provoke expression ‐β target genes vitro . Materials and Methods were soaked aqueous extracts fresh demineralized chips placed cell culture medium....

The aim of this retrospective study was to determine the prevalence early implant failure using a single system and identify factors contributing failure.Patients who received treatment with (Luna®, Shinhung, Seoul, Korea) at Dankook University Dental Hospital from 2015 2017 were enrolled. following data collected for analysis: sex age patient, seniority surgeon, diameter length implant, position in dental arch, access approach sinus-floor elevation, type guided bone regeneration (GBR)...

Abstract Background The aim of this case‐control study was to estimate the diagnostic accuracy standard clinical parameters in diagnosing healthy peri‐implant tissues, mucositis, and peri‐implantitis. Methods A designed compare used diagnosis diseases such as: probingdepth (PD), bleeding on probing (BOP), mucosal redness (MR), suppuration (SUP), plaque index (PI). Furthermore, influence patient‐ (sex, age) implant‐related variables (implant neck configuration, time function after loading)...

Abstract Guided bone regeneration (GBR) often utilizes a combination of autologous grafts, deproteinized bovine mineral (DBBM), and collagen membranes. DBBM membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated composition BCM its activity on osteogenic mesenchymal stromal cells. We detected fast significant ( P...

Autografts are considered to support bone regeneration. Paracrine factors released from cortical might contribute the overall process of graft consolidation. The aim this study was characterize paracrine by means proteomic analysis.Bone-conditioned medium (BCM) prepared fresh chips porcine mandibles and subjected analysis. Proteins were categorized clustered using bioinformatic tools UNIPROT PANTHER, respectively.Proteomic analysis showed that BCM contains more than 150 proteins, which 43...

Autologous bone is used for augmentation in the course of oral implant placement. Bone grafts release paracrine signals that can modulate mesenchymal cell differentiation vitro. The detailed genetic response bone-derived fibroblasts to these has remained elusive. Paracrine accumulate bone-conditioned medium (BCM) prepared from porcine cortical chips.In this study, were exposed BCM followed by a whole genome expression profiling and downstream quantitative reverse transciptase polymerase...

A short inflammatory phase and fast ingrowth of blood vessels mesenchymal cells are essential for tissue integration a biomaterial. Macrophages play key role in this process. We investigated invasion macrophages, vessels, proliferating into highly porous volume-stable collagen matrix (VCMX) used soft augmentation around teeth dental implants. The biomaterial was implanted submucosal pouches the canine maxilla, response analyzed at six different time points. Immunohistochemistry done (PCNA),...

Collagen-based scaffolds hold great potential for tissue engineering, since they closely mimic the extracellular matrix. We investigated integration of an engineered porous collagen-elastin scaffold developed soft augmentation. After implantation in maxillary submucosal pouches 6 canines, cell invasion (vimentin), matrix deposition (collagen type I) and degradation (cathepsin k, tartrate-resistant acid phosphatase (TRAP), CD86) were (immuno)-histochemically evaluated. Invasion vimentin+...

Abstract Objective Polyetheretherketone (PEEK) is a popular synthetic thermoplastic polymer for medical applications, but its clinical use suffers from several limitations. Therefore, the aim was to compare soft tissue response dental implant closure caps made of PEEK or titanium as evaluated by occurrence multinucleated giant cells (MNGCs). Material and methods Forty‐two implants were placed in maxilla seven miniature pigs. While commercially pure (Ti) had Ti cap, ceramic either zirconia...

Background: Demineralized bone matrix (DBM) is used for the treatment of osseous defects. Conditioned medium from native chips can activate transforming growth factor (TGF)‐β signaling in mesenchymal cells. The aim this study to determine whether processing into DBM affects activity conditioned medium. Methods: Porcine cortical blocks were subjected defatting, different concentrations hydrochloric acid, and various temperatures. was lyophilized, ground, placed culture Human gingiva...

Abstract Background The use of enamel matrix derivative ( EMD ) has been shown to facilitate periodontal regeneration by histologically resulting in formation cementum, ligament and bone. Recently, a new liquid carrier system for introduced with better physicochemical properties specifically designed bone graft mixing (Osteogain). aim this study was investigate the combination Osteogain bovine‐derived natural mineral NBM on osteoblast migration, adhesion, proliferation differentiation....

The use of autogenous bone chips during guided regeneration procedures has remained the gold standard for grafting due to its excellent combination osteoconduction, osteoinduction and osteogenesis. Recent protocols established by our group have characterized specific growth factors cytokines released from that potential be harvested isolated into conditioned media (BCM). Due advantageous osteo-promotive properties BCM, aims present study was pre-coat collagen barrier membranes with BCM...

Regenerative therapies are commonly needed prior to implant placement in the posterior maxilla following tooth extraction. The aim of this study was investigate anatomical features atrophic that might affect surgical approach selection and technique risk intra- postoperative complications. Cone beam computed tomography files were screened identify pristine maxillary ridges need bone augmentation (ridge height < 10 mm). Ridge width lateral wall thickness (LWT) measured at different levels,...

Chemical decontamination increases the availability of bone grafts; however, it remains unclear whether antiseptic processing changes biological activity bone.Bone chips were incubated with four different solutions including (1) povidone-iodine (0.5%), (2) chlorhexidine diguluconate (0.2%), (3) hydrogen peroxide (1%) and (4) sodium hypochlorite (0.25%). After 10 min. incubation, in capacity bone-conditioned medium (BCM) to modulate gene expression gingival fibroblasts was...

Abstract Objectives Osteoclasts rapidly form on the surface of bone chips at augmentation sites. The underlying molecular mechanism, however, is unclear. Soluble factors released from in vitro have a robust impact mesenchymal cell differentiation. Whether these soluble change differentiation hematopoietic cells into osteoclasts remains unknown. Methods Osteoclastogenesis, formation tartrate‐resistant acid phosphatase‐positive multinucleated cells, was studied with murine marrow cultures...

Introduction: Common in vitro cell culture systems for testing implant material immune compatibility either rely on immortal human leukocyte lines or isolated primary cells. Compared to vivo conditions, this generates an environment of substantially reduced complexity, often lacking important types, such as neutrophil granulocytes and others. The aim study was establish a reliable test system materials under vivo-like conditions. Methods: Test were incubated closed, CO 2 -independent,...