A5055003008- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Meta-analysis and systematic reviews
- COVID-19 Clinical Research Studies
- Congenital heart defects research
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Cardiac, Anesthesia and Surgical Outcomes
- Electrospun Nanofibers in Biomedical Applications
- Clinical practice guidelines implementation
- Glaucoma and retinal disorders
- Autoimmune Bullous Skin Diseases
- Cardiomyopathy and Myosin Studies
- Bone Tissue Engineering Materials
- Graphene and Nanomaterials Applications
- Contact Dermatitis and Allergies
- Silymarin and Mushroom Poisoning
- Dermatological diseases and infestations
- Analytical Chemistry and Chromatography
- Radiology practices and education
- Clinical Reasoning and Diagnostic Skills
- Peptidase Inhibition and Analysis
- Digital Imaging in Medicine
- Single-cell and spatial transcriptomics
McMaster University
2024-2025
Washington University in St. Louis
2022-2024
UNSW Sydney
2019-2024
University of North Carolina at Chapel Hill
2024
Impact
2024
Northwestern University
2022-2024
Shirley Ryan AbilityLab
2024
Stanford University
2017-2023
Cardiovascular Institute of the South
2017-2023
Association Clinique et Thérapeutique Infantile du Val de Marne
2023
Background: The progression toward low-cost and rapid next-generation sequencing has uncovered a multitude of variants uncertain significance (VUS) in both patients asymptomatic “healthy” individuals. A VUS is rare or novel variant for which disease pathogenicity not been conclusively demonstrated excluded, thus cannot be definitively annotated. VUS, therefore, pose critical clinical interpretation risk-assessment challenges, new methods are urgently needed to better characterize their...
Abstract Introduction Understanding the changing epidemiology of adults hospitalized with coronavirus disease 2019 (COVID-19) informs research priorities and public health policies. Methods Among (≥18 years) laboratory-confirmed, acute COVID-19 between 11 March 2021, 31 August 2022 at 21 hospitals in 18 states, those during severe respiratory syndrome 2 (SARS-CoV-2) Omicron-predominant period (BA.1, BA.2, BA.4/BA.5) were compared to from earlier Alpha- Delta-predominant periods. Demographic...
Perhaps one of the most significant achievements in modern science is discovery human induced pluripotent stem cells (hiPSCs), which have paved way for regeneration therapy using patients' own cells. Cardiomyocytes differentiated from hiPSCs (hiPSC-CMs) could be used modelling patients with heart failure, testing new drugs, and cellular future. However, present cardiomyocyte differentiation protocols exhibit variable efficiency across different hiPSC lines, inhibit application this...
Rationale: Previous studies have shown that human embryonic stem cell-derived cardiomyocytes improved myocardial recovery when administered to infarcted pig and non-human primate hearts.However, the engraftment of intramyocardially delivered cells is poor effectiveness clinically relevant doses induced pluripotent (hiPSC-CMs) in large animal models injury remains unknown.Here, we determined whether thymosin β4 (Tb4) could improve reparative potency transplanted hiPSC-CMs a porcine model...
The fundamental question of which genes are most important in controlling liver regeneration remains unanswered. We employed a parallel screen to test the impact 43 selected on repopulation Fah(-/-) mouse model hereditary tyrosinemia. discovered that transcription factor Foxa3 was strong promoter regeneration, while tumor necrosis receptor 1 (TNFR1) significant suppressor among all tested. Our approach enabled identification these factors as regulators and potential drug targets for...
Abstract Purpose Large language models have shown promise in answering questions related to medical conditions. This study evaluated the responses of ChatGPT-4 patient-centred frequently asked (FAQs) relevant age-related macular degeneration (AMD). Methods Ten experts across a range clinical, education and research practices optometry ophthalmology. Over 200 patient-centric FAQs from authoritative professional society, hospital advocacy websites were condensed into 37 four themes:...
A short inflammatory phase and fast ingrowth of blood vessels mesenchymal cells are essential for tissue integration a biomaterial. Macrophages play key role in this process. We investigated invasion macrophages, vessels, proliferating into highly porous volume-stable collagen matrix (VCMX) used soft augmentation around teeth dental implants. The biomaterial was implanted submucosal pouches the canine maxilla, response analyzed at six different time points. Immunohistochemistry done (PCNA),...
Collagen-based scaffolds hold great potential for tissue engineering, since they closely mimic the extracellular matrix. We investigated integration of an engineered porous collagen-elastin scaffold developed soft augmentation. After implantation in maxillary submucosal pouches 6 canines, cell invasion (vimentin), matrix deposition (collagen type I) and degradation (cathepsin k, tartrate-resistant acid phosphatase (TRAP), CD86) were (immuno)-histochemically evaluated. Invasion vimentin+...
Mutational outcomes following CRISPR-Cas9-nuclease cutting in mammalian cells have recently been shown to be predictable and, certain cases, skewed toward single genotypes. However, the ability control these remains limited, especially for 1-bp insertions, a common and therapeutically relevant class of repair outcomes. Here, through small molecule screen, we identify ATM kinase inhibitor KU-60019 as compound capable reproducibly increasing fraction insertions relative other Cas9 Small or...
The application of teledermatology for evaluating acne patients has yielded comparable therapeutic outcomes with traditional face-to-face evaluation, but follow-up compliance between these modalities is not well-studied. Our objective to compare the rate and duration initially evaluated by versus in-person outpatient consultation. Electronic medical review patients, 18-35 years-old seen via evaluation at University Pittsburgh Medical Center 2010-2018 was performed. Teledermatology were less...
Introduction: Neuroendocrine/small‐cell prostate cancer (NEPC) is a rare and aggressive subtype of cancer, which typically develops after prolonged treatment for metastatic castration‐resistant disease, but can, less commonly, occur de novo. Case Presentation: We describe case novo NEPC in tumor with mixed pathology including acinar adenocarcinoma neuroendocrine/small‐cell carcinoma rapid progression disease. Despite initiation androgen deprivation therapy (ADT) chemotherapy, the patient...
Rationale: Regulatory DNA elements in the human genome play important roles determining transcriptional abundance and spatiotemporal gene expression during embryonic heart development somatic cell reprogramming. It is not well known how chromatin marks regulatory are modulated to establish type–specific heart. Objective: We aimed decipher epigenetic signatures they modulate heart-specific expression. Methods Results: profiled genome-wide activity a variety of using massive RNA-seq (n=12)...
To evaluate the agreement and accuracy of grading goniophotographs anterior segment optical coherence tomography (AS-OCT) results for assessment chamber angle, elicit factors driving concordance between perceived grade ground truth.Three clinicians evaluated AS-OCT 75 patients. Graders' impressions angle grade, trabecular pigmentation, iris contour were compared with truth gonioscopic examination result when physically performed by a senior optometrist. Percentage kappa statistics...
Introduction The aim of this methodological review is to evaluate the completeness reporting surgical pilot and feasibility randomised trials as per Consolidated Standards Reporting Trials (CONSORT) extension trials. Moreover, we assess for presence spin inconsistency between abstract main text in Methods analysis A comprehensive, electronic search strategy will be used identify studies indexed Medline, Embase Cochrane Central Register Controlled (CENTRAL) databases. Studies included if they...
This study presents a method based on acid transesterification and the purification by solid-phase extraction (SPE) coupled with gas chromatography–tandem mass spectrometry for quantifying 3- 2-monochloropropanediol esters (3-MCPDE, 2-MCPDE) glycidyl (GE) in nutritional foods. The fat was extracted liquid–liquid petroleum ether diethyl after sample hydrolysed ammonia. Then extract purified SPE cartridge filled aminopropyl sorbents. It demonstrated that optimal elution volume 3-MCPDE, 2-MCPDE...