A5057601507- MicroRNA in disease regulation
- Metabolism, Diabetes, and Cancer
- Cancer-related molecular mechanisms research
- Bone Metabolism and Diseases
- Protease and Inhibitor Mechanisms
- Pancreatic function and diabetes
- Lung Cancer Treatments and Mutations
- High-Temperature Coating Behaviors
- Allergic Rhinitis and Sensitization
- RNA modifications and cancer
- Nuclear Materials and Properties
- Tracheal and airway disorders
- Protein Kinase Regulation and GTPase Signaling
- Bone and Dental Protein Studies
- Extracellular vesicles in disease
- Food Allergy and Anaphylaxis Research
- Pregnancy and preeclampsia studies
- Molecular Biology Techniques and Applications
- Circular RNAs in diseases
- Diabetes and associated disorders
- Adipose Tissue and Metabolism
- Renal Diseases and Glomerulopathies
- Metallurgical Processes and Thermodynamics
- Minerals Flotation and Separation Techniques
- Lung Cancer Diagnosis and Treatment
Kyoto University
2025
Nippon Medical School
2009-2024
Osaka Prefecture University
2014-2024
Osaka Metropolitan University
2022-2024
Tsuchiura Kyodo General Hospital
2024
National Hospital Organization Mito Medical Center
2003-2023
Tohoku University
2021-2023
Fuji Oil (Japan)
2011-2018
Health & Life (Taiwan)
2017
Institute of Macromolecular Chemistry
2016
In this study, we performed small RNA library sequencing using human placental tissues to identify placenta-specific miRNAs. We also tested the hypothesis that chorionic villi could secrete miRNAs extracellularly via exosomes, which in turn enter into maternal circulation. By sequencing, most (e.g., MIR517A) were linked a miRNA cluster on chromosome 19. The genes differentially expressed development. Subsequent validation by real-time PCR and situ hybridization revealed villous trophoblasts...
In this study, to search for novel preeclampsia (PE) biomarkers, we focused on microRNA expression and function in the human placenta complicated with PE. By comprehensive analyses of expression, identified 22 microRNAs significantly upregulated preeclamptic placentas, 5 which were predicted silico commonly target mRNA encoding hydroxysteroid (17-β) dehydrogenase 1 (HSD17B1), a steroidogenetic enzyme expressed predominantly placenta. vivo HSD17B1 at both protein levels, was decreased...
MicroRNAs (miRNAs) are endogenous non-coding small RNAs that can regulate the expression of complementary mRNA targets. Identifying tissue-specific miRNAs is first step toward understanding biological functions miRNAs, which include regulation tissue differentiation and maintenance identity. In this study, we performed RNA library sequencing in adult mouse testis ovary to reveal their characteristic organ- gender-specific profiles elucidate characteristics expressed reproductive system. We...
Biliary tract cancer (BTC) is often difficult to diagnose definitively, even through histological examination. MicroRNAs (miRNAs) regulate a variety of physiological processes. In recent years, it has been suggested that profiles for circulating miRNAs, as well those tissue have the potential be used diagnostic biomarkers cancer. The aim this study was confirm existence miRNAs in human bile and assess their clinical BTC. We sampled from patients who underwent biliary drainage diseases such...
Interaction domains located in the NH2 terminus of IRS-1 mediate its recognition by insulin receptor. Alignment and IRS-2 reveals two homology regions: IH1PH contains a pleckstrin (PH) domain, IH2PTB phosphotyrosine binding (PTB) domain. A third region called SAIN was proposed to contain another functional PTB Peptide competition experiments demonstrated that IRS-2, like corresponding domain IRS-1, binds directly peptides containing NPXY motifs. In contrast, these do not bind or regions. 32D...
Cathepsin K is a recently identified cysteine protease which abundantly and selectively expressed in osteoclasts. To evaluate the contribution of cathepsin to bone resorption processes, we investigated effect antisense phosphothiorate oligodeoxynucleotide (S-ODN) on bone-resorbing activity Rabbit osteoclasts were cultured dentine slices for 24 h presence or absence S-ODN medium containing 100 nM Tfx™-50, polycationic liposome, as carrier S-ODN. Uptake by was confirmed microscopically using...
Ligaments and tendons are comprised of tough yet flexible connective tissue.Little is known, however, about the precise characteristics cells in ligaments due to absence specific markers cell lines.We recently reported a periodontal ligament line, PDL-L2, with suppressed Runx2/Osf2 transcriptional activity an inability form mineralized nodules.The present study demonstrates that homeobox protein Msx2 key factor suppressing those two functions.Msx2 colocalizes suppresses its cooperatively,...
The periodontal ligament (PDL) is a connective tissue located between the cementum of teeth and alveolar bone mandibula. It plays an integral role in maintenance regeneration tissue. cells responsible for maintaining this are thought to be fibroblasts, which can either multipotent or composed heterogenous cell populations. However, as no established lines from PDL available, it difficult assess what type promotes all these functions. As first step circumvent problem, we have cloned...
Tumor necrosis factor (TNF)-α is one of the candidate mediators insulin resistance associated with obesity, a major risk for development type 2 diabetes. The induced by TNF-α antagonized thiazolidinediones (TZDs), new class insulin-sensitizing drugs. aim current study was to dissect mechanism whereby pioglitazone, TZDs, ameliorates TNF-α–induced in 3T3-L1 adipocytes. Pioglitazone restored insulin-stimulated 2-deoxyglucose (DOG) uptake, which reduced TNF-α, concomitant restorations tyrosine...
Abstract Mechanical stress is an important factor controlling bone remodeling, which maintains proper morphology and functions. However, the mechanism by mechanical transduced into biological stimuli remains unclear. Therefore, purpose of this study to examine how gene expression changes with osteoblast differentiation cells differentiate osteoblasts. Tensile was applied cranial suture neonatal mouse calvaria in a culture means helical springs. The extended gradually, displaying marked...
It was previously considered that protein knockdown using a combination of zinc finger degron tag and thalidomide analog is impossible in mouse cells, however, EGFP expression as an indicator, we found possible super-degron (SD) with iberdomide or mezigdomide cells. Despite the efficient degradation SD-tagged wild-type PD-1 could not be degraded but human Jurkat cells human-type CEREBLON (CRBNI391V). In mice CRBNI391V, endogenous tagged SD efficiently suppressed T both cultured whole body....
Intrahepatic cholangiocarcinoma (ICC), which arises in the small bile ducts of liver, is second most common liver malignancy. Although modulation microRNA (miRNA) expression has been shown to be a potent sign malignant tumors, miRNA profiles ICC remains unclear. We performed sequencing analysis RNA libraries 2 cell lines (HuCCT1 and MEC) one normal intrahepatic biliary epithelial line (HIBEpiC) produce vitro. Furthermore, by means real-time polymerase chain reaction (PCR) we validated...
In spite of the undisputed importance altered expression patterns microRNAs (miRNAs) in various cancers, there is little information on clinicopathologic significance cancer-related miRNAs (MIR21, MIR143, MIR144, MIR145, and MIR205) esophageal squamous cell carcinoma (ESCC). We examined levels precursor mature miRNA genes ESCC using real-time polymerase chain reaction (PCR). also investigated mRNA processing elements (RNASEN, DGCR8, DICER1) that participate miRNA-biogenesis pathway....
Using a yeast two-hybrid system, we identified several proteins that interact with the PH domains in IRS-1 and IRS-2, including Lon protease, myeloblast protein, nucleolin. Although roles of these molecules insulin action are not yet known, each protein contained an acidic motif interacted domain IRS-2. However, only nucleolin bound to IRS-1, suggesting IRS-2 identical. Moreover, synthetic peptides based on protease inhibited binding but confirming selectivity domains. The ability bind...
To investigate the difference of insulin action between skeletal muscle and adipose tissue in response to dietary manipulation, we studied effect high-sucrose (HS) high-fat (HF) diet on by measuring binding both soleus muscles adipocytes. HS feeding led a 14 28% decrease, HF 25 36% decrease adipocytes, respectively (P less than 0.01). In HF-fed rats, rates glucose uptake intracellular metabolism were impaired 30-40% 0.01), adipocyte was also decreased 30% submaximally insulin-stimulated...
Abstract The periodontal ligament (PDL), a connective tissue located between the cementum of teeth and alveolar bone mandibula, plays crucial role in maintenance regeneration tissues. PDL contains fibroblastic cells heterogeneous cell population, from which we have established several lines previously. To analyze characteristics unique for at molecular level, performed cDNA microarray analysis versus MC3T3‐E1 osteoblastic cells. followed by validation reverse transcription‐polymerase chain...
Small interfering RNA (siRNA)-based interference (RNAi) is widely used for target gene silencing in various organisms. We previously showed that 8-nt-long 5′ proximal nucleotides, which include seed sequence (positions 2–8 from the end of guide strand), and complementary passenger strand are capable being simultaneously replaced with cognate deoxyribonucleotides without any substantial loss silencing. In present study, examination was made requirements non-seed region siRNA. The siRNA found...