Because very large numbers of single nucleotide polymorphisms (SNPs) are now available throughout the genome, they particularly suitable for detection genomic regions where a reduction in heterozygosity has occurred and offer new opportunities to improve accuracy inbreeding ([Formula: see text]) estimates. Runs homozygosity (ROH) contiguous lengths homozygous segments genome two haplotypes inherited from parents identical. Here, we investigated occurrence distribution ROH using medium-dense...

Runs of homozygosity (ROH) are widely used as predictors whole-genome inbreeding levels in cattle. They identify regions that have an unfavorable effect on a phenotype when homozygous, but also the genes associated with traits economic interest present these regions. Here, distribution ROH islands and enriched within four dairy cattle breeds were investigated. Cinisara (71), Modicana (72), Reggiana (168) Italian Holstein (96) individuals genotyped using 50K v2 Illumina BeadChip. The genomic...

Copy number variation (CNV) is a major source of genomic structural variation. The aim this study was to detect CNV regions (CNVR) in Valle del Belice dairy sheep population and identify those affecting milk production traits. GO analysis identified possible candidate genes pathways related the selected We CNVs 416 individuals genotyped using Illumina OvineSNP50 BeadChip array. association correlation-trend test model examined with Golden Helix SVS 8.7.0 tool. Significant were detected when...

The utilization of dairy by-products as animal feed, especially in swine production, is a strategy to provide functional ingredients improve gut health. This study explored the potential effect liquid whey-supplemented diet on fecal microbiota eleven pigs belonging Nero Siciliano breed. Five were assigned control group and fed with standard formulation whereas six experimental same feed supplemented whey. Fecal samples collected from each individual before (T0), one (T1) two (T2) months...

Caciocavallo Silano PDO is a traditional Italian stretched-curd cheese produced in southern Italy, subjected to minimum ripening period of 30 days. The present study aimed characterize the chemical composition and fatty acid (FA) profile at three stages (up 120 days). proximate composition, FAs profile, lactose content samples from production batches, all made with Friesian cow milk, were analyzed. results showed significant compositional changes during maturation. Moisture decreased 46.5%...

The application of genomic technologies has facilitated the assessment inbreeding based on single nucleotide polymorphisms (SNPs). In this study, we computed several runs homozygosity (ROH) parameters to investigate patterns using Illumina Goat SNP50 in five Italian local populations: Argentata dell’Etna (N = 48), Derivata di Siria 32), Girgentana 59), Maltese 16) and Messinese 22). ROH results showed well-defined differences among populations. A total 3687 segments >2 Mb were detected...

Copy number variants (CNVs) are one of the major contributors to genetic diversity and phenotypic variation in livestock. The aim this work is identify CNVs perform, for first time, a CNV-based population genetics analysis with five Italian sheep breeds (Barbaresca, Comisana, Pinzirita, Sarda, Valle del Belìce). We identified 10,207 an average length 1.81 Mb. showed similar mean numbers CNVs, ranging from 20 (Sarda) 27 (Comisana). A total 365 CNV regions (CNVRs) were determined. CNVRs varied...

This study assessed the potential effect of a co-feed liquid whey-integrated diet on fecal microbiota 14 crossbred pigs. The experimental design was as follows: seven pigs were in control group, fed with feed, and same feed supplemented daily whey. collection samples conducted each animal before dietary treatment (T0) one (T1), two (T2) months after beginning integration. In addition, blood collected from pig at time points order to evaluate physiological parameters. Taxonomic analysis...

Red grape Nero d’Avola cultivar pomace powder (GPP) was applied during fresh ovine cheese production in order to increase polyphenol content. Before cheeses were produced, the bacteria of a freeze-dried commercial starter culture isolated and tested vitro against GPP. Two dominant strains, both resistant GPP, identified. Thestarter inoculated pasteurized ewe’s milk curd divided into two bulks, one added with 1% (w/w) GPP another GPP-free. did not influence development, since lactic acid...

Summary Nero Siciliano (Sicilian Black, SB) is a local pig breed generally of uniform black color. In addition to this officially recognized breed, there are animals showing morphological characteristics resembling the SB but with gray hair Grey, SG). The SG, compared SB, also shows more compact structure greater transverse diameters, higher average daily gains and lower thickness back fat. study, using Illumina PorcineSNP60 BeadChip, we run genome‐wide analyses identify regions that may...

In the Mediterranean basin countries, dairy sheep production is usually based on local breeds, which are very well-adapted to their systems and environments can indeed guarantee income, employment, economic viability in areas where alternatives scarce or non-existent. Mastitis still one of greatest problems affecting commercial milk production. However, genetic evaluation mastitis particularly difficult because its low heritability categorical nature trait. The aim this study was identify...

Summary Genome‐wide association studies ( GWAS es) have become a powerful tool for identifying genomic regions associated with important traits in livestock. Milk production dairy sheep are measured at different time points during their life span. Using phenotypic data generated from longitudinal could improve the power of but until now received less attention es as methodology and has not been implemented. The aim this study was to carry out milk Valle del Belice using repeated measures....

Summary Microtia is a congenital deformity of the outer ear with phenotypes varying from small auricle to total absence (anotia). The genetic basis still poorly understood, and very few studies have been performed in sheep. Valle del Belice sheep breed showing microtia. aim this study was identify potential genomic regions involved microtia A 40 individuals, 20 normal, were genotyped Illumina Ovine SNP 50 BeadChip. comparison among results genome‐wide association study, Fisher's exact test F...

Copy-number variations (CNVs) are one of the widely dispersed forms structural in mammalian genomes, and present as deletions, insertions, or duplications. Only few studies have been conducted goats on CNVs derived from SNP array data, many local breeds still remain uncharacterized, e.g., Sicilian goat dairy breeds. In this study, CNV detection was performed, starting genotypic data 120 individuals, belonging to four (Argentata dell'Etna, Derivata di Siria, Girgentana, Messinese), genotyped...

Differential somatic cell count (DSCC), the percentage of (SCC) due to polymorphonuclear leukocytes (PMNs) and lymphocytes (LYMs), is a promising effective diagnostic marker for dairy animals with infected mammary glands. Well-explored in cows, DSCC also potentially valid sheep, where clinical subclinical mastitis outbreaks are among principal causes culling. We pioneered application ewes by applying receiver-operating characteristic (ROC) curve analysis define most accurate thresholds...

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The aim of the present study was to identify copy-number variations (CNVs) in Cinisara (CIN) and Modicana (MOD) cattle breeds on basis signal intensity (logR ratio) B allele frequency each marker, using Illumina’s BovineSNP50K Genotyping BeadChip. CNVs were detected with PennCNV SVS 8.7.0 software aggregated into CNV regions (CNVRs). identified 487 CIN that 86 CNVRs, 424 MOD 81 CNVRs. a total 207 39 181 41 CNVRs two softwares contained 29 common 17 MOD. Only small number have been elsewhere,...

The knowledge of milk proteome has been greatly enhanced by technological advances in the proteomics field as use two-dimensional differential in-gel electrophoresis, a gel-based approach which allowed analysis proteins from complex mixtures and comparing several protein samples same experiment. aim this study was to characterise whole proteomic profile Girgentana dairy goat breed elecrophoresis. obtained representative 2D map showed general picture distributions over pH 3–10 NL including...

Contagious agalactia (CA), a disease caused by Mycoplasma agalactiae and other pathogenic mycoplasmas, is well-known multietiological syndrome affecting dairy breeds of sheep goats in the Mediterranean basin. The aim this work was to study effect on milk production composition mastitis M. Valle del Belice sheep. All ewes were manually milked twice daily from both milking analysed for somatic cell counts. Moreover morning samples collected aseptically each animal bacteriological analyses. A...

Abstract The aim of this study was to identify genomic regions underlying milk production traits in the Valle del Belice dairy sheep using regional heritability mapping (RHM). Repeated measurements for yield (MY), fat percentage and (F% FY) protein (P% PY), collected over a period 6 years (2006–2012) on 481 ewes, were used analysis. Animals genotyped with Illumina 50k SNP chip. Variance components, heritabilities repeatabilities within across lactations estimated, fitting parity, litter...