A5100347940- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Essential Oils and Antimicrobial Activity
- Bacterial biofilms and quorum sensing
- Listeria monocytogenes in Food Safety
- Parasitic Infections and Diagnostics
- Vibrio bacteria research studies
- Insect Resistance and Genetics
- Polyamine Metabolism and Applications
- Microbial Metabolism and Applications
- Entomopathogenic Microorganisms in Pest Control
- Neuroscience and Neuropharmacology Research
- Antimicrobial Resistance in Staphylococcus
- Salmonella and Campylobacter epidemiology
- Animal Virus Infections Studies
- Immune Response and Inflammation
- Diet and metabolism studies
- Aquaculture disease management and microbiota
- Virology and Viral Diseases
- Insect and Pesticide Research
- Escherichia coli research studies
- CRISPR and Genetic Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Digestive system and related health
- Nicotinic Acetylcholine Receptors Study
First Affiliated Hospital of Jiangxi Medical College
2024
Nanchang University
2024
Huazhong Agricultural University
2008-2023
First Affiliated Hospital of Kunming Medical University
2021
Kunming Medical University
2021
Jinan University
2014
Chinese Academy of Sciences
2012
State Key Laboratory of Agricultural Microbiology
2008
We have developed a strategy for isolating cry genes from Bacillus thuringiensis. The key steps are the construction of DNA library in an acrystalliferous B. thuringiensis host strain and screening formation crystal through optical microscopy observation sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses. By this method, three genes--cry55Aa1, cry6Aa2, cry5Ba2--were cloned rice-shaped crystals, producing YBT-1518, which consists 54- 45-kDa proteins. cry55Aa1...
Listeria monocytogenes is a ubiquitous bacteria and causative agent of zoonotic listeriosis with high mortality. The consumption contaminated animal-derived foods has been linked both epidemic sporadic listeriosis. In this work, total 64 L. isolates from 259 pork samples sold in 11 supermarket chains were identified characterized by comparative whole-genome analysis. All delineated into eight clonal complexes (CCs), namely CC2, CC8, CC9, CC11, CC155, CC121, CC204, CC619, spanning two...
ABSTRACT In Pseudomonas aeruginosa , the quorum-sensing (QS) system is closely related to biofilm formation. We previously demonstrated that 14-alpha-lipoyl andrographolide (AL-1) has synergistic effects on antibiofilm and antivirulence factors (pyocyanin exopolysaccharide) of P. when combined with conventional antibiotics, while it little inhibitory effect its growth. However, molecular mechanism remains elusive. Here we investigated AL-1 QS systems, especially Las Rhl systems. This...
While a high osmolarity medium activates Cpx signaling and causes CpxR to repress csgD expression, efflux protein TolC plays an important role in biofilm formation Escherichia coli, whether also responds change regulate extraintestinal pathogenic E. coli (ExPEC) remains unknown. In this study, we constructed ΔtolC mutant complement ExPEC strains investigate the of retention curli production capability under different osmotic conditions. The showed significantly decreased lost ability produce...
Riemerella anatipestifer (RA), a major causative agent of septicemia anserum exsudativa in domesticated ducklings, has protein secretion system known as the type IX (T9SS). It is unknown whether T9SS contributes to virulence RA through factors associated with pathogenesis. To answer this question, we constructed an mutant deficient sprT, which encodes core T9SS. Deletion sprT yielded cells that failed digest gelatin, effect was rescued via complementation by plasmid encoding wild-type sprT....
Riemerella anatipestifer, an avian pathogen, has resulted in enormous economic losses to the duck industry globally. Notwithstanding, little is known regarding physiological, pathogenic and virulence mechanisms of anatipestifer (RA) infection. However, role Ferric uptake regulator (Fur) R. not, date, been demonstrated. Using a genetic approach, unmarked gene deletion system, we evaluated function fur anatipestifer. For this purpose, constructed suicide vector containing pheS as counter...
Our previous study showed that the inactivation of efflux pump TolC could abolish biofilm formation and curli production extraintestinal pathogenic Escherichia coli (ExPEC) strain PPECC42 under hyper-osmotic conditions. In this we investigated role OmpX in ExPEC PPECC42. data disruption or overexpression didn't significantly affect wild-type strain. However, tolC-deleted mutant, overexpressing suppressed effect on growth Real-time qRT-PCR confirmed affected by regulating transcription...
Staphylococcus aureus is one of the major foodborne pathogens and can cause serious illness in humans by foods contaminated with S. enterotoxins. In recent years, livestock-associated has been a public health concern for emerged various countries globally. China largest producers pigs pork world. However, there are few studies on detailed genotypic pathogenic characterization pork-associated China. this study, prevalence, antimicrobial resistance, characteristics raw Wuhan, China, were...
The Gram-negative bacterium Riemerella anatipestifer is an important waterfowl pathogen, causing major economic losses to the duck-producing industry. However, little known of virulence factors that mediate pathogenesis during R. infection. In this study, RAYM_RS09735 and RAYM_RS09740 were predicted form a two-component signaling system (TCS) through bioinformatics analysis. This TCS was highly conserved across Flavobacteriaceae. A mutant YMΔRS09735/RS09740 strain constructed investigate...
We previously demonstrated that the growth of poorly differentiated nasopharyngeal carcinoma cells (CNE-2Z) was more dependent on activities volume-activated chloride channels than normal epithelial (NP69-SV40T). However, and roles such in highly (CNE-1) are not clarified. In this study, it found a current regulatory volume decrease (RVD) were induced by 47% hypotonic challenges. The density capacity RVD CNE-1 lower those CNE-2Z cells, higher channel blockers, 5-nitro-2-(3-phenylpropylamino)...
Listeria monocytogenes is a facultative intracellular foodborne pathogen that can cause listeriosis in humans and animals. L. LM201 was isolated from foodstuff. The draft genome sequence of strain provides the genetic basis for application this biotechnological vaccine production.
Abstract Aim: To reveal the changes of intestinal microbial abundance and composition, as well microbiota metabolic levels bile acids short chain fatty healthy preschool children during their growth. Methods: Feces 120 newborns 150 aged 6 months to years were collected. Then composition flora was analyzed by 16S rRNA, contents in feces detected LC-MS GS methods, respectively. Results: The function microflora not stable neonatal period but significantly improved at after birth, gradually...