A5086881458- Ubiquitin and proteasome pathways
- Parkinson's Disease Mechanisms and Treatments
- Endoplasmic Reticulum Stress and Disease
- Alzheimer's disease research and treatments
- Drug Transport and Resistance Mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- Viral Infections and Immunology Research
- Plant-Microbe Interactions and Immunity
- Cancer-related Molecular Pathways
- Protein Structure and Dynamics
- Tea Polyphenols and Effects
- Biochemical and Molecular Research
- Neuroscience and Neuropharmacology Research
- Viral gastroenteritis research and epidemiology
- Enzyme Structure and Function
- Nematode management and characterization studies
- Heavy metals in environment
- Legume Nitrogen Fixing Symbiosis
- Preterm Birth and Chorioamnionitis
- Biotin and Related Studies
- Genetics and Neurodevelopmental Disorders
- Pregnancy and preeclampsia studies
- Enzyme Production and Characterization
- Chromium effects and bioremediation
- Hepatitis Viruses Studies and Epidemiology
Regional Centre for Biotechnology
2015-2025
University of Burdwan
2013-2024
Nagoya Institute of Technology
2012
Purdue University West Lafayette
2010-2012
Brandeis University
2010
Indian Institute of Technology Kharagpur
2003-2009
Weizmann Institute of Science
2009
Presidency University
2006
The Bay of Bengal is known as the epicenter for seeding several devastating cholera outbreaks across globe. Vibrio cholerae , etiological agent cholera, has extraordinary competency to acquire exogenous DNA by horizontal gene transfer (HGT) and adapt them into its genome structuring metabolic processes, developing drug resistance, colonizing human intestine. Antimicrobial resistance (AMR) in V. become a global concern. However, little about identity traits, source AMR genes, acquisition...
Abstract (−)‐Epigallocatechin‐3‐gallate (EGCG), the major constituent of green tea has been reported to prevent many diseases by virtue its antioxidant properties. The binding EGCG with human serum albumin (HSA) investigated for first time using fluorescence, circular dichroism (CD), Fourier transform infrared (FTIR) spectroscopy, and protein‐ligand docking. We observed a quenching fluorescence HSA in presence EGCG. parameters were determined Scatchard plot results found be consistent those...
Ubiquitin carboxy-terminal hydrolase L1 (UCHL1) is a Parkinson disease-associated, putative cysteine protease found abundantly and selectively expressed in neurons. The crystal structure of apo UCHL1 showed that the active-site residues are not aligned canonical form, with nucleophilic being 7.7 Å from general base histidine, an arrangement consistent inactive form enzyme. Here we report structures wild type two disease-associated variants enzyme, S18Y I93M, bound to ubiquitin-based suicide...
Abstract Ubiquitin C-terminal Hydrolase-1 (UCHL1) is a deubiquitinating enzyme, which plays key role in Parkinson’s disease (PD). It one of the most important proteins, constitute Lewy body PD patient. However, how this well folded highly soluble protein presents proteinaceous aggregate still unclear. We report here that UCHL1 undergoes S-nitrosylation vitro and rotenone induced mouse model. The preferential nitrosylation Cys 90, 152 220 has been observed alters catalytic activity structural...
Background: Atypical hemolytic uremic syndrome (aHUS), an important cause of acute kidney injury (AKI), is characterized by dysregulation the alternative complement pathway. Autoantibodies to factor H (FH), a chief regulator this pathway, account for distinct subgroup. While high anti-FH titers predict relapse, they do not correlate well with disease activity and their functional characterization required. Methods: Of 781 patients <18-year-old aHUS in nationwide database from 2007 2018, 436...
Lymphatic filariasis, frequently caused from Wuchereria bancrofti infection, is endemic in several parts of the globe and responsible for human health problems socioeconomic loss to a large extent. Inflammatory consequences originating host-parasite interaction play major role disease pathology allied complications. The identity key mediator this process yet unknown filarial research.Microfilarial protein (MfP) was isolated sheath W. microfilariae through ultrafiltration, followed by...
Abstract BRCA1 associated protein 1 (BAP1) is a nuclear deubiquitinase that regulates tumor suppressor activity and widely involves many cellular processes ranging from cell cycle regulation to gluconeogenesis. Impairment of enzymatic localization induce abnormal proliferation. It considered be an important driver gene, which undergoes frequent mutations in several cancers. However the role mutation oncogenic gain function BAP1 are poorly understood. Here, we investigated localization,...
Emergence of antimicrobial resistant Gram-negative bacteria has created a serious global health crisis and threatens the effectiveness most, if not all, antibiotics commonly used to prevent treat bacterial infections. There is dearth detailed studies on prevalence resistance (AMR) patterns in India. Here, we have isolated examined AMR 654 enteric pathogens investigated complete genome sequences isolates from six representative genera, which aggregate encode against 22 representing nine...
α-Synuclein is a natively unfolded protein and its deposition in the Lewy body neurites substantia nigra region of brain linked to Parkinson's disease (PD). The molecular mechanisms α-synuclein aggregation clearance have not been well understood. Until now, several strategies designed inhibit related cytotoxicity. Polyphenols, small molecules, synthetic peptides, peptide-derived molecules considered as potential candidates that oligomerization fibrillation, few them are clinical trials. We...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a positive-strand RNA virus. The viral genome capped at the 5' end, followed by an untranslated region (UTR). There poly(A) tail 3' preceded UTR. self-interaction between regulatory elements present within and UTRs their interaction with host/virus-encoded proteins mediate function of UTRs. Using RNA-protein detection (RaPID) assay coupled to liquid chromatography tandem mass spectrometry, we identified host partners SARS-CoV-2...
Preterm birth (PTB) refers to the delivery of a baby before completion 37 weeks gestation. It is significant global health issue with implications for both mothers and neonates. The placenta transient organ crucial in sustenance pregnancy until parturition; its dysfunction associated different adverse outcomes, including PTB. We conducted nested case-control study 40 placental tissue samples from preterm term deliveries their comparative protein profiles. Label-free quantitation (LFQ)...
Insulin fibrillation inflicts both economic and clinical challenges by causing bioactivity loss, inflammation, adverse effects during storage, transport, injection. The present study explores antiamyloidogenic fibril-disaggregating of a gut microbiota-derived indole metabolite, indole-3-acetic acid (IAA) on insulin fibrillation. According to Thioflavin T (ThT) fluorescence assays transmission electron microscopy (TEM), IAA significantly inhibited primary seed-induced insulin. We note that...
Rationally applied, structurally guided computational methods hold the promise of identifying potent and distinct chemotypes while enabling precise targeting structural determinants. Here, we implemented a workflow combining insights from cocrystal poses monitoring dynamical determinants our previous studies for identification potential candidates against USP7. We identified tested several diverse chemical scaffolds, which underwent in vitro validation across six cancer cell lines. Among...
Ubiquitin carboxy-terminal hydrolase L5 (UCHL5) is a proteasome-associated deubiquitinating enzyme, which, along with RPN11 and USP14, known to carry out deubiquitination on proteasome. As member of the ubiquitin (UCH) family, UCHL5 unusual because, unlike UCHL1 UCHL3, it can process polyubiquitin chain. However, does so only when bound proteasome; in its free form, capable releasing relatively small leaving groups from C-terminus ubiquitin. Such behavior might suggest at least two...
Abstract Nutrient surplus and consequent free fatty acid accumulation in the liver cause hepatosteatosis. The exposure of acids to cultured hepatocyte hepatocellular carcinoma cell lines induces cellular stress, organelle adaptation, subsequent death. Despite many studies, mechanism associated with lipotoxicity death still remains poorly understood. Here, we have used proteomics approach circumvent for using cells as a model. Our quantitative data revealed that ectopic lipids severely...