Pyometra, accumulation of pus in the uterus, is a bacterial infection that frequently initiates systemic inflammation. The disease may have lethal consequences when effects are severe or complications occur. Markers for identifying high-risk patients and predicting outcome therefore high demand. objective this study was to measure serum concentrations insulin-like growth factor-I (IGF-I), iron, C-reactive protein (CRP), amyloid A (SAA) bitches with pyometra explore possible value these...

Abstract Background Osteoarthritis (OA) is a common, age-related joint disease in cats. The common finding of bilateral symmetric involvement may suggest influence from systemic factors, and could imply that body parameters such as excess fat cat size are important for OA development. We aimed to investigate associations between composition whole-body scores 72 cats, using computed tomography (CT), if serum concentrations the metabolic mediators leptin, adiponectin insulin-like growth...

ABSTRACT Background There is limited knowledge of urine analytes in different stages chronic kidney disease (CKD) dogs. Objectives To study markers and fractional excretion (FE) dogs CKD a healthy control group (C). Animals Fifty various 30 Methods In this cross‐sectional observational study, presenting to referral hospital given diagnosis using standard methods, dogs, were included. Urinary cystatin C ( uCysC ), glucose uGlu protein uProt creatinine uCr urea uUrea sodium uNa potassium uK...

The applications of data on biological variation include assessment the utility population-based reference intervals, evaluation significance change in serial results, and setting analytical quality specifications. We investigated 19 biochemistry analytes total T4, measured serum from 7 clinically healthy domestic cats sampled once weekly for 5 weeks. Samples were frozen analyzed random order same run. Results outliers, components variance, subsequently generated by restricted maximum...

Background Feline insulin has been measured previously using assays developed for measuring human insulin. As feline differs from insulin, it is important to validate the assay before use. Objectives The aims of this study were an ELISA , Mercodia Insulin intended and determine stability in serum. Methods Validation which uses monoclonal antibodies that recognize both included evaluation coefficients variation ( CV s), patterns variation, consistency after dilution spiking with Stability was...

Abstract Background Obesity is associated with insulin resistance (IR) and considered a risk factor for diabetes mellitus (DM) in cats. It has been proposed that homeostasis model assessment (HOMA-IR), which the product of fasting serum (mU/L) glucose (mmol/L) divided by 22.5, can be used to indicate IR. The objectives this study were threefold: (i) evaluate associations between body fat, insulin, HOMA-IR, (ii) determine population-based reference interval HOMA-IR healthy lean cats, (iii)...

Background In the dog, normal estrous cycle includes a prolonged luteal phase. Progesterone stimulates local canine mammary growth hormone ( GH ) production, which may act systemically and contribute to insulin resistance. Swedish Elkhounds are predisposed progesterone‐related diabetes mellitus, relationship among resistance, , insulin‐like factor I IGF ‐I) is of particular interest. Objective To study resistance in relation ‐I nondiabetic during diestrus. We also assessed whether...

Background Insulin‐like growth factor I ( IGF ‐I) measurements are used in veterinary medicine for diagnosing hormone disorders. ‐I assays subject to interference by ‐binding proteins IGFBP ) which may not be efficiently removed standard extraction methods. Adding excess ‐ II during analysis improve accuracy. Objectives The purpose of the study was validate a commercial human ELISA uses feline samples and evaluate biologic variation. Methods Precision determined calculating coefficient...

Today immunoassays are widely used in veterinary medicine, but lack of species specific assays often necessitates the use developed for human applications. Mass spectrometry (MS) is an attractive alternative due to high specificity and versatility, allowing species-independent analysis. Targeted MS-based quantification methods valuable complements large scale shotgun A method referred as parallel reaction monitoring (PRM), implemented on Orbitrap MS, has lately been presented excellent more...

Background Insulin‐like growth factor‐I (IGF‐I) is used to screen for acromegaly in diabetic cats. In humans, most circulating IGF‐I forms ternary complexes (TC) with IGF‐binding protein (IGFBP‐3) and an acid‐labile subunit. Compared the amount of TC cats more variable. concentrations are reported increase during insulin treatment, rapidly achieving remission. Objectives To investigate (i) factors associated concentrations, including IGFBP‐profiles (ii) effect treatment on (iii) as...

Nonislet-cell tumor hypoglycemia (NICTH) is a rare paraneoplastic phenomenon well described in dogs and humans. Tumors associated with NICTH secrete incompletely processed forms of insulin-like growth factor-II (IGF-II), commonly named big IGF-II. These have increased bioavailability interact the insulin IGF-I receptor causing growth-promoting effects. Immunoassays designed for human samples been used to measure canine -II, but they possess some limitations. In addition, there are no...

Feline diabetes mellitus is a common endocrine disease with increasing prevalence. It shows similarities human type 2 and characterized by insulin resistance deficient secretion. Moreover, cats humans belong to the very few species that form amyloid depositions in pancreatic islets. However, little known about cat islet function no studies have addressed secretion from isolated islets ex vivo. The aim of this study was establish protocol for isolation Langerhans pancreata euthanized due...

Measurement of canine pancreatic lipase immunoreactivity (cPLI) is used for diagnosing pancreatitis in dogs. Because can be a life-threatening disease with severe complications, an in-house cPLI test would valuable to obtain rapid results. The aim this study was evaluate point-of-care test, Vcheck cPL. Precision, determined according EP15, and linearity under dilution were judged against preset quality goals. Results from the cPL compared previously validated ELISA, Spec In retrospective...

Biological variation (BV) of urinary (U) biochemical analytes has not been described in absolute terms, let alone as a ratio the U-creatinine or fractional excretion healthy dogs. These are potential diagnostic tools for different types kidney damage and electrolyte disorders dogs.We aimed to investigate BV specific gravity, osmolality, creatinine, urea, protein, glucose, chloride, sodium, potassium, calcium, phosphate urine from pet dogs.Blood samples 13 dogs were collected once weekly 8...

Abstract Venous blood gases were analyzed with ABL90 FLEX in two cats Heinz bodies approximately 60% of the erythrocytes. The instrument demonstrated an inability to correctly report standard bicarbonate (stHCO 3 − ), hematocrits, and hemoglobin concentrations by indicating OXI spectrum mismatch alarm (ie, measured forms differed from calculated forms). Actual (aHCO ) did not indicate any errors. uses spectrophotometry measure hemoglobin, presence interfered measurement these cases. Because...

The prevalence of diabetes mellitus (DM) in cats is increasing. It has been proposed that fasting concentrations insulin and glucose can be used for screening reduced sensitivity cats. Reduced may indicate are at risk developing DM motivate early preventive actions such as weight reduction change diet. To correctly interpret results it important to know the biological variation i.e. normal between within For glucose, coefficient (CV) reported 8.1 6.8%, respectively, but until now there no data

Insulin independence is a realistic therapeutic goal in feline diabetes. Remission dependent on achieving fast glycemic control. However, without intensive monitoring high insulin doses increase the risk of hypoglycemia. An 11-year old spayed Burmese cat presented with diabetes after steroid treatment for skin allergy. Control blood glucose was not achieved using low carbohydrate diet plus recommended q12h either lente or glargine, total dose limited by hypoglycaemia.