A5027428460- Neurobiology and Insect Physiology Research
- Developmental Biology and Gene Regulation
- Invertebrate Immune Response Mechanisms
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Gene Regulatory Network Analysis
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Genetics, Aging, and Longevity in Model Organisms
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Advanced Biosensing Techniques and Applications
- Animal Behavior and Reproduction
- Plant Molecular Biology Research
- Circular RNAs in diseases
- Cancer Mechanisms and Therapy
- Monoclonal and Polyclonal Antibodies Research
- Cancer Cells and Metastasis
- Insect and Arachnid Ecology and Behavior
- interferon and immune responses
- Cancer Research and Treatments
- Chromatin Remodeling and Cancer
- RNA and protein synthesis mechanisms
- HER2/EGFR in Cancer Research
MSD (Switzerland)
2023
ETH Zurich
2017-2021
Hunan Academy of Traditional Chinese Medicine
2021
University of Basel
2012-2017
University of Zurich
2014
Members of the SWI/SNF chromatin-remodeling complex are among most frequently mutated genes in human cancer, but how they suppress tumorigenesis is currently unclear. Here, we use Drosophila neuroblasts to demonstrate that component Osa (ARID1) prevents by ensuring correct lineage progression stem cell lineages. We show induces a transcriptional program transit-amplifying population initiates temporal patterning, limits self-renewal, and dedifferentiation. identify Prdm protein Hamlet as key...
The number of neurons generated by neural stem cells is dependent upon the regulation cell proliferation and programmed death. Recently, novel that amplify through intermediate progenitors, called type II neuroblasts, have been discovered, which are active during brain development in Drosophila. We investigated death dorsomedial (DM) amplifying lineages contribute to central complex Drosophila, using clonal mosaic analysis with a repressible marker (MARCM) lineage-tracing techniques. A...
Antibody–drug conjugates (ADCs), integrating high specificity of antigen-targeting antibodies and potency cell-killing chemical drugs, have become one the most rapidly expanding therapeutic biologics in oncology. Although ADCs were widely studied from multiple aspects, overall structural elucidation with comprehensive understanding variants is scarcely reported. Here, for first time, we present a holistic in-depth characterization an interchain cysteine-conjugated ADC, focusing on...
Tumor initiation is often linked to a loss of cellular identity. Transcriptional programs determining identity are preserved by epigenetically-acting chromatin factors. Although such regulators among the most frequently mutated genes in cancer, it not well understood how an abnormal epigenetic condition contributes tumor onset. In this work, we investigated gene signature tumors caused disruption Drosophila regulator, polyhomeotic (ph). larval tissue ph mutant cells show shift towards...
Advances in synthetic biology have enabled robust control of cell behavior by using tunable genetic circuits to regulate gene expression a ligand-dependent manner. Such can be used direct the differentiation pluripotent stem cells (PSCs) towards desired types, but rational design PSCs is challenging due variable intracellular environment. Here, we provide framework for implementing switches based on combinations transcriptional, structural, and posttranslational elements that engineered as...
Abstract Polycomb group proteins are epigenetic regulators maintaining transcriptional memory during cellular proliferation. In Drosophila larvae, malfunction of Polyhomeotic (Ph), a member the PRC1 silencing complex, results in neoplastic growth. Here, we report an intrinsic tumour suppression mechanism mediated by steroid hormone ecdysone metamorphosis. Ecdysone alters growth into nontumorigenic state mutant ph cells which then become eliminated adult stage. We demonstrate that exerts this...
Stem cells need to balance self-renewal and differentiation for correct tissue development homeostasis. Defects in this can lead developmental defects or tumor formation. In recent years, mRNA splicing has emerged as an important mechanism regulating cell fate decisions. Here we address the role of evolutionarily conserved co-factor Barricade (Barc)/Tat-SF1/CUS2 Drosophila neural stem (neuroblast) lineage We show that Barc is required generation neurons during brain by ensuring progenitor...
Neurogenesis is initiated by a set of basic Helix-Loop-Helix (bHLH) transcription factors that specify neural progenitors and allow them to generate neurons in multiple rounds asymmetric cell division. The Drosophila Daughterless (Da) protein its mammalian counterparts (E12/E47) act as heterodimerization for proneural genes are therefore critically required neurogenesis. Here, we demonstrate Da can also be an inhibitor the progenitor fate whose absence leads stem overproliferation tumor...
In the developing fruit fly brain, a protein called Trithorax increases number of neural cells produced from single stem cell, in part by regulating transcription target genes buttonhead and pointed.
Objective: To study the biological behavior of nasopharyngeal carcinoma stem cells and to explore activation Ras signaling pathway regulated by CD44. Methods: CNE2-SC 5-8F-SC were obtained serum-free suspension culture. Cell counting kit-8 (CCK-8) assay, colony formation Transwell migration cell adhesion array used investigate growth, proliferation, cells. Western blot test was detect expressions related proteins siRNA-mediated interference determine Results: The growth rates significantly...
Summary Long non-coding (lnc) RNAs contain functional elements that play important regulatory roles in a variety of processes during development, normal physiology, as well disease. We recently discovered new lncRNA, we named let-A , expressed from the evolutionary conserved let-7-Complex locus Drosophila . This RNA induces cell death cancer cells. Here show ectopic expression is also exerting an oncolytic toxicity several human lines, but shows almost no effect more differentiated or lines...
Abstract The let-7 complex in Drosophila encodes three evolutionarily conserved microRNAs: miR-100, , and miR-125 . These act as heterochronic genes regulating developmental timing response to the steroid hormone ecdysone play important roles cell differentiation. Here we identify two additional long non-coding RNAs complex, named let-A let-B Both are transcribed large first intron of primary RNA encoding microRNAs. We show these be sequentially expressed early pupal stages signaling, albeit...